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Dergiler / Hacettepe Journal of Biology and Chemistry / 2009 / Cilt: 37 - Sayı: 3

In vitro effects of dexamethasone on human serum paraoxonase-I (PON1) activity

Orhan ATEŞ
Şükrü BEYDEMİR
Deniz EKİNCİ
Sayfa
197–205
Yayın Tarihi
2022-11-05
DOI
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Our results show that dexamethasone inhibits hPON1 activity under in vitro conditions.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Dexamethasone has successfully been used for treatment of some inflammatory diseases.
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Temel Bulgular & Veri:Here, we evaluated the in vitro effects of dexamethasone on the activity of human serum paraoxonase (hPON1).
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Sonuç & Katkı:Our results show that dexamethasone inhibits hPON1 activity under in vitro conditions.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

Dexamethasone has successfully been used for treatment of some inflammatory diseases. Here, we evaluated the in vitro effects of dexamethasone on the activity of human serum paraoxonase (hPON1). PON1 enzyme was purified using simple chromatographic methods: DEAE-Sephadex anion exchanger and Sephadex G 200 gel filtration chromatography from human serum. hPON1 was purified approx. 225-fold with a final specific activity of 4867.3 U x $mg^ {-1}$ proteins and with a purity up to 39.8%. Dexamethasone dose-dependently decreased in vitro hPON1 activity. IC50 value for dexamethasone was determined as 1.106. Inhibition constant (Ki) was estimated as 3.284 ± 0.394 mM from Lineweaver-Burk graph. Inhibition type of dexamethasone was determined as noncompetitive. Our results show that dexamethasone inhibits hPON1 activity under in vitro conditions.

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Bu Makaleye Atıf Yap

ATEŞ, O., BEYDEMİR, Ş., EKİNCİ, D. (2022). In vitro effects of dexamethasone on human serum paraoxonase-I (PON1) activity. Hacettepe Journal of Biology and Chemistry, Cilt: 37 - Sayı: 3, 197–205. https://ajindex.com/articles/651232

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Kaynakça (14)

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