SavedAdd your journal

Journals / Hacettepe Journal of Biology and Chemistry / 2009 / Cilt: 37 - Sayı: 3

In vitro effects of dexamethasone on human serum paraoxonase-I (PON1) activity

Orhan ATEŞ
Şükrü BEYDEMİR
Deniz EKİNCİ
Pages
197–205
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our results show that dexamethasone inhibits hPON1 activity under in vitro conditions.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Dexamethasone has successfully been used for treatment of some inflammatory diseases.
📊
Key Findings & Data:Here, we evaluated the in vitro effects of dexamethasone on the activity of human serum paraoxonase (hPON1).
💡
Conclusion & Impact:Our results show that dexamethasone inhibits hPON1 activity under in vitro conditions.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Dexamethasone has successfully been used for treatment of some inflammatory diseases. Here, we evaluated the in vitro effects of dexamethasone on the activity of human serum paraoxonase (hPON1). PON1 enzyme was purified using simple chromatographic methods: DEAE-Sephadex anion exchanger and Sephadex G 200 gel filtration chromatography from human serum. hPON1 was purified approx. 225-fold with a final specific activity of 4867.3 U x $mg^ {-1}$ proteins and with a purity up to 39.8%. Dexamethasone dose-dependently decreased in vitro hPON1 activity. IC50 value for dexamethasone was determined as 1.106. Inhibition constant (Ki) was estimated as 3.284 ± 0.394 mM from Lineweaver-Burk graph. Inhibition type of dexamethasone was determined as noncompetitive. Our results show that dexamethasone inhibits hPON1 activity under in vitro conditions.

🔖

Cite This Article

ATEŞ, O., BEYDEMİR, Ş., EKİNCİ, D. (2022). In vitro effects of dexamethasone on human serum paraoxonase-I (PON1) activity. Hacettepe Journal of Biology and Chemistry, Cilt: 37 - Sayı: 3, 197–205. https://ajindex.com/articles/651232

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Beydemir, Ş., Kulaçoğlu, D.N., Çiftçi, M., Küfrevioğlu, Ö.İ., The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase, Turk. J. Chem., 27, 601, 2003
  2. Ekinci, D., Beydemir, Ş., Alım, Z., Some drugs inhibit in vitro hydratase and esterase activities of human carbonic anhydrase-I and II, Pharmacol. Rep., 59, 580, 2007
  3. Aharoni, A., Gaidukov, L., Khersonsky, O., Gould, S.M., Roodveldt, C., The ‘evolvability’ of promiscuous protein functions, Nat. Genet., 37, 73, 2005
  4. Alici, H.A., Ekinci, D, Beydemir, Ş., Intravenous anesthetics inhibit human paraoxonase-I (PON1) activity in vitro and in vivo, Clin. Biochem., 41(16-17) 1384, 2008
  5. Khersonsky, O., Tawfik, D.S., Structure-reactivity studies of serum paraoxonase PON1 suggest that its native activity is lactonase, Biochemistry-US., 44, 6371, 2005
  6. Rochua, D., Chabriere, E., Massona, P., Human paraoxonase: A promising approach for pre-treatment and therapy of organophosphorus poisoning, Toxicology, 233, 47, 2007
  7. Akgur, S.A., Ozturk, P., Solak, I., Moral, A.R., Human serum paraoxonase (PON1) activity in acute organophosphorus insecticide poisoning, Forensic Sci. Int., 133, 136, 2003
  8. Billecke, S., Draganov, D., Councell, R., Stetson, P., Watson, C., Hsu, C., La Du, B.N., Human serum paraoxonase (PON1) isozymes Q and R hydrolyze lactones and cyclic carbonate esters, Drug Metab. Dispos., 28, 1335, 2000
  9. Davies, H.G., Richter, R.J., Keifer, M., et al., The effect of the human serum paraoxonase polymorphism is reversed with diazoxon, soman and sarin, Nat. Genet., 14, 334, 1996
  10. La Du, B.N., Human serum paraoxonase/arylesterase. In Pharmacogenetics of Drug Metabolism (Kalow, W., ed.), Pergamon Elmford, NY, pp: 51–91, 1992
  11. Abbott, C.A., Mackness, M.I., Kumar, S., Boulton, A.J., Durrington, P.N. Serum paraoxonase activity, concentration and phenotype distribution in diabetes mellitus and its relationship to serum lipids and lipoproteins, Arterioscl. Throm. Vas., 15, 1812, 1995
  12. Baskol, G., Demir, H., Baskol, M., Kilic, E., Ates, F., Kocer, D., Muhtaroglu, S. Assessment of Paraoxonase 1 activity and malondialdehyde levels in patients with rheumatoid arthritis, Clin. Biochem., 38, 951, 2005
  13. Baskol, G., Karakucuk, S., Oner, A.O., Baskol, M., Kocer, D., Mirza, E., Saraymen, R., Ustdal, M., Serum paraoxonase 1 activity and lipid peroxidation levels in patients with age-related macular degeneration, Ophthalmologica, 220, 12, 2006
  14. Dantoine, T.F., Deb