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Dergiler / Turkish Journal of Biology / 2022 / Cilt: 46 - Sayı: 3

Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells

Bünyamin AKGÜL
Syed Muhammad HAMID
Sayfa
216–226
Yayın Tarihi
2022-10-18
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

These results suggest that 5’-tRFs are cytoplasmically-localized and regulate gene expression through inhibition of translation in Drosophila.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Transfer ribonucleic acids (tRNAs) serve not only as amino acid carriers during translation but also as a template for the biogenesis of short fragments that can regulate gene expression.
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Temel Bulgular & Veri:We used synthetic tRFs to investigate their localization and function in Drosophila S2 cells.
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Sonuç & Katkı:These results suggest that 5’-tRFs are cytoplasmically-localized and regulate gene expression through inhibition of translation in Drosophila.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

Transfer ribonucleic acids (tRNAs) serve not only as amino acid carriers during translation but also as a template for the biogenesis of short fragments that can regulate gene expression. Despite recent progress in the function of tRNA-derived fragments (tRFs), their intracellular localization, protein partners, and role in regulating translation are not well understood. We used synthetic tRFs to investigate their localization and function in Drosophila S2 cells. Under our experimental setting, all synthetic tRFs tested were localized at distinct sites within the cytoplasm in a similar manner in Drosophila S2 cells. Cytoplasmically-localized tRFs were positioned in close proximity to GW182 and XRN1 proteins. Functionally, tRFs, which slightly suppressed proliferation in S2 cells, inhibited translation without any major shift in the polysome profile. These results suggest that 5’-tRFs are cytoplasmically-localized and regulate gene expression through inhibition of translation in Drosophila.

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Bu Makaleye Atıf Yap

AKGÜL, B., HAMID, S. M. (2022). Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells. Turkish Journal of Biology, Cilt: 46 - Sayı: 3, 216–226. https://ajindex.com/articles/600079

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Kaynakça (2)

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