SavedAdd your journal

Journals / Turkish Journal of Biology / 2022 / Cilt: 46 - Sayı: 3

Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells

Bünyamin AKGÜL
Syed Muhammad HAMID
Pages
216–226
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results suggest that 5’-tRFs are cytoplasmically-localized and regulate gene expression through inhibition of translation in Drosophila.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Transfer ribonucleic acids (tRNAs) serve not only as amino acid carriers during translation but also as a template for the biogenesis of short fragments that can regulate gene expression.
📊
Key Findings & Data:We used synthetic tRFs to investigate their localization and function in Drosophila S2 cells.
💡
Conclusion & Impact:These results suggest that 5’-tRFs are cytoplasmically-localized and regulate gene expression through inhibition of translation in Drosophila.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Transfer ribonucleic acids (tRNAs) serve not only as amino acid carriers during translation but also as a template for the biogenesis of short fragments that can regulate gene expression. Despite recent progress in the function of tRNA-derived fragments (tRFs), their intracellular localization, protein partners, and role in regulating translation are not well understood. We used synthetic tRFs to investigate their localization and function in Drosophila S2 cells. Under our experimental setting, all synthetic tRFs tested were localized at distinct sites within the cytoplasm in a similar manner in Drosophila S2 cells. Cytoplasmically-localized tRFs were positioned in close proximity to GW182 and XRN1 proteins. Functionally, tRFs, which slightly suppressed proliferation in S2 cells, inhibited translation without any major shift in the polysome profile. These results suggest that 5’-tRFs are cytoplasmically-localized and regulate gene expression through inhibition of translation in Drosophila.

🔖

Cite This Article

AKGÜL, B., HAMID, S. M. (2022). Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells. Turkish Journal of Biology, Cilt: 46 - Sayı: 3, 216–226. https://ajindex.com/articles/600079

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (2)

  1. Balagopal V, Parker R (2009).
  2. Polysomes, P bodies and stress granules: states and fates of eukaryotic mRNAs. Current Opinion in Cell Biology 21: 403-408. doi: 10.1016/j.ceb.2009.03.005 Bariarz JE, Ruby JG, Wang Y, Bartel DP, Blelloch R (2008). Mouse ES cells express endogenous shRNAs, siRNAs and other microprocessor-independent Dicer-dependent small RNAs. Genes and Development 22: 2773-2785. doi: 10.1101/ gad.1705308 Cole C, Sobala A, Lu C, Thatcher SY, Bowman A et al. (2009). Filtering of deep-sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs. RNA 15: 2147-2160. doi: 10.1261/rna.1738409 Cosacak MI, Yigit H, Kizil C, Akgül B (2018). Re-arrangements in the cytoplasmic distribution of small RNAs following the maternal-to-zygotic transition in Drosophila embryos. Genes 9: 82. doi: 10.3390/genes9020082 Couvillion MT, Bounova G, Purdom E, Speed TP, Collins K (2012). A Tetrahymena Piwi bound to mature tRNA 3’ fragments activates the exonuclease Xrn2 for RNA processing in the nucleus. Molecular Cell 48: 509-520. doi: 10.1016/j. molcel.2012.09.010 Emara MM, Ivanov P, Hickman T, Dawra N, Tisdale S et al. (2010). Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly. Journal of Biological Chemistry 285: 10959-10968. doi: 10.1074/jbc. M109.077560 Gebetsberger J, Polacek N (2013). Slicing tRNAs to boost functional ncRNA diversity. RNA Biology 10: 1798-1806. doi: 10.4161/ rna.27177 Gebestberger J, Zywicki M, Kunzi A, Polacek N (2012). tRNAderived fragments target the ribosome and function as regulatory non-coding RNA in Haloferax volcanii. Archaea 260909. doi: 10.1155/2012/260909 Goodarzi H, Liu X, Nguyen HCB, Zhang S, Fish L et al. (2015). Endogenous tRNA-derived fragments suppress breast cancer progression via YBX1 displacement. Cell 161: 790-802. doi: 10.1016/j.cell.2015.02.053 Göktaş C, Yigit H, Cosacak MI, Akgul B (2017). Differentially expressed tRNA-derived small RNAs co-sediment primarily with non-polysomal fractions in Drosophila. Genes 8: 333. doi: 10.3390/genes8110333 Eystathioy T, Jakmiw A, Chan EK, Seraphin B, Cougot N et al. (2003). The GW182 protein colocalizes with mRNA degradation associated proteins Dcp1 and hLSm4 in cytoplasmic GW bodies. RNA 9: 1171-1173. doi: 10.1261/rna.5810203 Haussecker D, Huang Y, Lau A, Paramaswatan P, Fire AZ et al. (2010). tRNA-derived small RNAs in the global regulation of RNA silencing. RNA 16: 673-695. doi: 10.1261/rna.2000810 Ivano