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Dergiler / Turkish Journal of Biology / 2018 / Cilt: 42 - Sayı: 2

Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis

İpek ERDOĞAN
Mehmet İlyas COŞACAK
Ayten NALBANT
Bünyamin AKGÜL
Sayfa
113–122
Yayın Tarihi
2018-04-27
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Altogether, these results suggest that miRNAs modulate apoptotic events in T cells and that cellular heterogeneity requires careful interpretation of miRNA expression profiles obtained from drug-treated cell lines.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:MicroRNAs (miRNAs) are small noncoding RNAs of about 19-25 nt that regulate gene expression posttranscriptionally under various cellular conditions, including apoptosis.
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Temel Bulgular & Veri:Our data showed that a total of 184 miRNAs were dysregulated.
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Sonuç & Katkı:Altogether, these results suggest that miRNAs modulate apoptotic events in T cells and that cellular heterogeneity requires careful interpretation of miRNA expression profiles obtained from drug-treated cell lines.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Apoptosis · microRNAs · Jurkat

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs of about 19-25 nt that regulate gene expression posttranscriptionally under various cellular conditions, including apoptosis. The miRNAs involved in modulation of apoptotic events in T cells are partially known. However, heterogeneity associated with cell lines makes it difficult to interpret gene expression signatures, especially in cancer-related cell lines. Treatment of the Jurkat T-cell leukemia cell line with the universal apoptotic drug, camptothecin, resulted in identification of two Jurkat subpopulations: one that is sensitive to camptothecin and another that is rather intrinsically resistant. We sorted apoptotic Jurkat cells from nonapoptotic ones prior to profiling miRNAs through deep sequencing. Our data showed that a total of 184 miRNAs were dysregulated. Interestingly, the apoptotic and nonapoptotic subpopulations exhibited distinct miRNA expression profiles. In particular, 6 miRNAs were inversely expressed in these two subpopulations. The pyrosequencing results were validated by real-time qPCR. Altogether, these results suggest that miRNAs modulate apoptotic events in T cells and that cellular heterogeneity requires careful interpretation of miRNA expression profiles obtained from drug-treated cell lines.

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Bu Makaleye Atıf Yap

ERDOĞAN, İ., COŞACAK, M. İ., NALBANT, A., AKGÜL, B. (2018). Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis. Turkish Journal of Biology, Cilt: 42 - Sayı: 2, 113–122. https://ajindex.com/articles/181348

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