COMET, TUNEL, and TEM analysis of an infertile male with short tail sperm

Amaç & Hipotez:Male infertility is correlated with sperm morphology and sperm DNA damage, which are completely different from that of fertile individuals.

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Temel Bulgular & Veri:Anaccurate sperm DNA damage analysis and ultrastructural examination of the ejaculate provide important support in the clinical evaluation.

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Sonuç & Katkı:Then, we evaluated the association between sperm DNAdamage and embryo quality.

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Alan Parametreleri

Abstract

Male infertility is correlated with sperm morphology and sperm DNA damage, which are completely different from that of fertile individuals. Anaccurate sperm DNA damage analysis and ultrastructural examination of the ejaculate provide important support in the clinical evaluation. It issupposed that in the near future, the fertilization rate, pregnancy rate, and miscarriages could be predicted using the combination of these typesof tests in assisted reproductive technologies (ARTs). For this purpose, we report a very rare case of an infertile man having short tail sperm. Theinfertile man and his wife underwent in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI). During this process, we examinedthe ultrastructure of the ejaculated sperm with transmission electron microscopy (TEM) and calculated the sperm DNA damage with terminaldeoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and COMET assays. Then, we evaluated the association between sperm DNAdamage and embryo quality.

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Bu Makaleye Atıf Yap

TAVMERGEN, E., GÜNDÜZ, C., DURMAZ, A., TAVMERGEN, E. N. G., DİKMEN, N., CİLAKER, S. M., BAĞRIYANIK, H. A., VATANSEVER, S. (2022). COMET, TUNEL, and TEM analysis of an infertile male with short tail sperm. Journal of the Turkish-German Gynecological Association, Cilt: 16 - Sayı: 1, 54–57. https://ajindex.com/articles/567544

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Kaynakça (1)

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