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Dergiler / Hittite Journal of Science and Engineering / 2019 / Cilt: 6 - Sayı: 1

Evaluation of Angiogenic Factor Release from Thermosensitive Poly(N-Vinylcaprolactam)-g-Collagen: In Vitro and In Vivo Studies

Serap DURKUT
Sayfa
57–61
Yayın Tarihi
2022-10-18
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

In vivo study confirmed that angiogenic-factor-loaded PNVCL-g-Col had the capacityto induce neovascularization indicating that the in vivo bioactivity of the VEGF waspreserved in the thermosensitive PNVCL-g-Col.

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Amaç & Hipotez:In this study, a thermosensitive poly(N-vinylcaprolactam)-g-collagen (PNVCL-g-Col)hybrid hydrogel was synthesized by conjugation using the NHS/EDC cross-linking system,and characterized.
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Temel Bulgular & Veri:At first, the efficiency of in vitro sustained delivery of human vascularendothelial growth factor (VEGF) from the thermosensitive PNVCL-g-Col hydrogelmodified with heparin, was evaluated for duration of ten days under in vitro physiologicalconditions (37oC, pH 7.4).
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Sonuç & Katkı:In vivo study confirmed that angiogenic-factor-loaded PNVCL-g-Col had the capacityto induce neovascularization indicating that the in vivo bioactivity of the VEGF waspreserved in the thermosensitive PNVCL-g-Col.
🔬Tür:Modelleme & Sayısal Simülasyon
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

In this study, a thermosensitive poly(N-vinylcaprolactam)-g-collagen (PNVCL-g-Col)hybrid hydrogel was synthesized by conjugation using the NHS/EDC cross-linking system,and characterized. At first, the efficiency of in vitro sustained delivery of human vascularendothelial growth factor (VEGF) from the thermosensitive PNVCL-g-Col hydrogelmodified with heparin, was evaluated for duration of ten days under in vitro physiologicalconditions (37oC, pH 7.4). The results indicated that PNVCL-g-Col hydrogel preservedits stability and released ~90% of the loaded VEGF within this time period. In vitro studyshowed that PNVCL-g-Col was basically histocompatible. Then, the in vivo angiogenicactivity of the VEGF-releasing PNVCL-g-Col was investigated using a subcutaneous ratmodel. In vivo study confirmed that angiogenic-factor-loaded PNVCL-g-Col had the capacityto induce neovascularization indicating that the in vivo bioactivity of the VEGF waspreserved in the thermosensitive PNVCL-g-Col.

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Bu Makaleye Atıf Yap

DURKUT, S. (2022). Evaluation of Angiogenic Factor Release from Thermosensitive Poly(N-Vinylcaprolactam)-g-Collagen: In Vitro and In Vivo Studies. Hittite Journal of Science and Engineering, Cilt: 6 - Sayı: 1, 57–61. https://ajindex.com/articles/538001

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