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Dergiler / Hittite Journal of Science and Engineering / 2026 / Volume: 6 Issue: 1

Evaluation of Angiogenic Factor Release From Thermosensitive Poly N-Vinylcaprolactam -g-Collagen: In Vitro and In Vivo Studies

Serap Durkut(Ankara University, Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara, Turkey)
Sayfa
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Yayın Tarihi
2019-03-28
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

In vivo study confirmed that angiogenic-factor-loaded PNVCL-g-Col had the capacity to induce neovascularization indicating that the in vivo bioactivity of the VEGF was preserved in the thermosensitive PNVCL-g-Col

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Amaç & Hipotez:I n this study, a thermosensitive poly N-vinylcaprolactam -g-collagen PNVCL-g-Col hybrid hydrogel was synthesized by conjugation using the NHS/EDC cross-linking system, and characterized.
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Temel Bulgular & Veri:At first, the efficiency of in vitro sustained delivery of human vascular endothelial growth factor VEGF from the thermosensitive PNVCL-g-Col hydrogel modified with heparin, was evaluated for duration of ten days under in vitro physiological conditions 37oC, pH 7.4 .
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Sonuç & Katkı:In vivo study confirmed that angiogenic-factor-loaded PNVCL-g-Col had the capacity to induce neovascularization indicating that the in vivo bioactivity of the VEGF was preserved in the thermosensitive PNVCL-g-Col
🔬Tür:Modelleme & Sayısal Simülasyon
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Thermosensitive polymer · Sustained release · Conjugation

Abstract

I n this study, a thermosensitive poly N-vinylcaprolactam -g-collagen PNVCL-g-Col hybrid hydrogel was synthesized by conjugation using the NHS/EDC cross-linking system, and characterized. At first, the efficiency of in vitro sustained delivery of human vascular endothelial growth factor VEGF from the thermosensitive PNVCL-g-Col hydrogel modified with heparin, was evaluated for duration of ten days under in vitro physiological conditions 37oC, pH 7.4 . The results indicated that PNVCL-g-Col hydrogel preserved its stability and released ~90% of the loaded VEGF within this time period. In vitro study showed that PNVCL-g-Col was basically histocompatible. Then, the in vivo angiogenic activity of the VEGF-releasing PNVCL-g-Col was investigated using a subcutaneous rat model. In vivo study confirmed that angiogenic-factor-loaded PNVCL-g-Col had the capacity to induce neovascularization indicating that the in vivo bioactivity of the VEGF was preserved in the thermosensitive PNVCL-g-Col

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Bu Makaleye Atıf Yap

Durkut, S. (2019). Evaluation of Angiogenic Factor Release From Thermosensitive Poly N-Vinylcaprolactam -g-Collagen: In Vitro and In Vivo Studies. Hittite Journal of Science and Engineering, Volume: 6 Issue: 1. https://doi.org/10.17350/HJSE19030000121

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Kaynakça (19)

  1. Hoffman AS. Stimuli-responsive polymers: Biomedical applications and challenges for clinical translation. Adv. Drug Deliv. Rev., 65, 10–16, 2013.
  2. Qiu Y, Park K. Environment-sensitive hydrogels for drug delivery. Adv. Drug Deliv. Rev., 64, 49–60, 2012.
  3. Peppas NA, Hilt JZ, Khademhosseini A. Hydrogels in biology and medicine: from molecular principles to bionanotechnology. Adv Mater;18:1345–60, 2006.
  4. Jeong B, Kim SW, Bae YH. Rev. Thermosensitive Sol−Gel Reversible Hydrogels. Adv. Drug Delivery, 64 (Supplement), 154−162, 2012.
  5. Cortez-Lemus NA, Licea-Claverie A. Poly(Nvinylcaprolactam), a comprehensive review on a thermoresponsive polymer becoming popular. Prog Polym Sci 53,1, 2016.
  6. Ferraz CC, Varca GHCC, Ruiz J, Lopes PS, Mathor MB, Lugão AB, Bucio E. Radiation-grafting of thermo- and pH-responsive poly(N-vinylcaprolactam-coacrylic acid) onto silicone rubber and polypropylene films for biomedical purposes, Radiat. Phys. Chem. 97, 298–303, 2014.
  7. Gillette BM, Jensen JA, Tang B. In situ collagen assembly for integrating microfabricated threedimensional cell-seeded matrices. Nat. Mater. 7(8):636–640, 2008.
  8. Klouda L, Mikos AG. Thermoresponsive hydrogels in biomedical applications. Eur. J. Pharm. Biopharm. 68, 34–45, 2008.
  9. Lee SC, Kwon IK, Park K. Hydrogels for delivery of bioactive agents: A historical perspective. Adv Drug Deliv Rev; 65:17–20, 2013.
  10. Chu H, and Wang Y. Therapeutic angiogenesis: controlled delivery of angiogenic factors. Ther. Deliv. 3, 693–714, 2012.
  11. Gu C, Zheng R, Yang Z, Wen A, Wu H, Zhang H, Yi D. Novel glycidyl methacrylated dextran/ gelatin nanoparticles loaded with basic fibroblast growth factor: Formulation and characteristics. Drug Dev Ind Pharm;35: 1419–142, 2009.
  12. Prabaharan M, Grailer JJ, Steeber DA, Gong S. Stimuli-Responsive Chitosan-graft-Poly(Nvinylcaprolactam) as a Promising Material for Controlled Hydrophobic Drug Delivery. Macromol. Biosci., 8, 843–851, 2008.
  13. Liu J, Debuigne A, Detrembleur C, Jerome C. Poly(Nvinylcaprolactam): a thermoresponsive macromolecule with promising future in biomedical field. Adv Healthc Mater. 3, 1941– 68, 2014.
  14. Wu JY, Liu SQ, Heng PWS, Yang YY. Evaluating proteins release from, and their interactions with, thermosensitive poly (N-isopropylacrylamide) hydrogels. J Control Release. 102:361–372, 2005.
  15. Durkut S, Elçin YM. Synthesis and characterization of thermosensitive poly(N-vinylcaprolactam)-g-collagen. Artificial Cells, Nanomedicine, and Biotechnology, 45: 8, 1665-1674, 2017.
  16. Nortona LW, Tegnella E, Toporek SS, Reichert WM. In vitro characterization of vascular endothelial growth factor and dexamethasone releasing hydrogels for implantable probe coatings. Biomaterials 26 3285–3297, 2005.
  17. Elcin EA and Elcin MY. Localized Angiogenesis Induced by Human Vascular Endothelial Growth Factor-Activated PLGA Sponge. Tissue Engineering 12(4):959-6, 2006.
  18. Pike DB, Cai S, Pomraning KR, Firpo MA, Fisher RJ, Shu XZ, Prestwich GD, Peattie RA. Heparin-regulated release of growth factors in vitro and angiogenic response in vivo to implanted hyaluronan hydrogels containing VEGF and bFGF. Biomaterials Volume 27, Issue 30, October, Pages 5242-5251, 2006.
  19. Peattie RA, Rieke ER, Hewett EM, Fisher RJ, Shu XZ, Prestwich GD. Dual growth factor-induced angiogenesis in vivo using hyaluronan hydrogel implants. Biomaterials, 27 (2006), pp. 1868-1875, 2006.