KaydedilenlerDerginizi ekleyin

Dergiler / Gazi University Journal of Science / 2018 / Cilt: 31 - Sayı: 4

Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane

Aydın ÖZLÜK
Naznoosh SHOMALI MOGHADDAM
Nursel GÜL
Ebru GÖKALP ÖZKORKMAZ
Sayfa
1022–1031
Yayın Tarihi
2022-10-19
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Our examinations revealed that D-galactose mostly exists in A.segetum larvae midgut cell membranes and peritrophic membrane and this means BPA and GSIlectins can be used as insecticidal agents against A. segetum.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Midgut cell and peritrophic membranes of grain pest Agrotis segetum larvae were examined bylight microscope.
📊
Temel Bulgular & Veri:Results of the studyindicated that lectins BPA (Bauhinia purpurea) and GS-I (Griffonia simplicifolia) stronglystained whereas, PNA (Pea Nut Agglutinin) and UEA-1 (Ulex europaeus) moderately stainedthe tissues.
💡
Sonuç & Katkı:Our examinations revealed that D-galactose mostly exists in A.segetum larvae midgut cell membranes and peritrophic membrane and this means BPA and GSIlectins can be used as insecticidal agents against A. segetum.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

Midgut cell and peritrophic membranes of grain pest Agrotis segetum larvae were examined bylight microscope. Six biotinylated lectins were used in the assays by applying histochemicalmethods (Avidin-Biotin-Peroxidase). The aim of this study was to find lectins binding onperitrophic membranes if they were available as insecticidal agents. Results of the studyindicated that lectins BPA (Bauhinia purpurea) and GS-I (Griffonia simplicifolia) stronglystained whereas, PNA (Pea Nut Agglutinin) and UEA-1 (Ulex europaeus) moderately stainedthe tissues. However, WGA (Wheat Germ Agglutinin) and Con-A (Canavalia ensiformis)stained the tissues weakly. The common feature of two binding proteins, BPA and GS-I lectinwas binding to D- galactose. Our examinations revealed that D-galactose mostly exists in A.segetum larvae midgut cell membranes and peritrophic membrane and this means BPA and GSIlectins can be used as insecticidal agents against A. segetum.

🔖

Bu Makaleye Atıf Yap

ÖZLÜK, A., MOGHADDAM, N. S., GÜL, N., ÖZKORKMAZ, E. G. (2022). Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane. Gazi University Journal of Science, Cilt: 31 - Sayı: 4, 1022–1031. https://ajindex.com/articles/521422

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (12)

  1. Zethner, O., Khan, B.M., Chaudhry, M.I., Bolet, B., Khan, S., Khan, H., Gul, H., Øgaard, L., Zaman, M., Nawaz, G., “Agrotis segetum granulosis virus as a control agent against field populations of Agrotis ipsilon and A. segetum (Lep: Noctuidae) on tobacco, okra, potato and sugar beet in northern Pakistan”, Entomophaga, 32 (5): 449-55, (1987).
  2. Erasmus, A., Van Rensburg, J.B., Van den Berg, J., “Effects of Bt maize on Agrotis segetum (Lepidoptera: Noctuidae): a pest of maize seedlings”, Environmental Entomology, 39(2): 702-06, (2010).
  3. Jakubowska, A.K., Dwight, E.L., Herrero, S., Vlak, J.M., Oers, M.M.V., “Host-range expansion of Spodoptera exigua multiple nucleopolyhedrovirus to Agrotis segetum larvae when the midgut is bypassed”, Journal of General Virology, 91: 898–906, (2010).
  4. Martoja, R., Ballan, C., The ultrastructure of the digestive and excetory organs, Insect Ultrastructure, R.C. King and H. Akai editors, Springer, Boston, (1984).
  5. Levy, S.M., Falleiros, M.F.A., Moscardi, F., Gregorio, E.A., Toledo, L., “Morphological study of the hindgut in larvea of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae)”, Neotropical Entomology, 33(4): 427-31, (2004a).
  6. Levy, S.M., Falleiros, M.F.A., Moscardi, F., Gregorio, E.A., Toledo, L., “The larval midgut of Anticarsia gemmatalis (Hübner) (Lepidoptera:Noctuidae): Light and electron microscopy studies of the epithelial cells”, Brazilian Journal of Biology, 64(3B): 633-38, (2004b).
  7. Lehane, M. J., “Peritrophic matrix structure and function”, Annual Review of Entomology, 42: 525-50, (1997).
  8. Tellam, R. L., Wijffels, G., Willadsen, P., “Peritrophic matrix proteins”, Insect Biochemistry and Insect Molecular Biology, 29: 87-101, (1999).
  9. Bolognesi, R., Terra, W.R., Ferreira, C., “Peritrophic membrane role in enhancing digestive efficiency: Theoretical and experimental models”, Journal of Insect Physiology, 54(10-11): 1413-22, (2008).
  10. Eisemann, C.H., Binnington, K.C., “The peritrophic membrane: its formation, structure, chemical composition and permeability in relation to vaccination against ectoparasitic arthropods”, International Journal of Parasitology, 24: 15-56, (1994).
  11. Merzendorfer, H., Zimoch, L., “Chitin metabolism in insects: structure, function and regulation of chitin synthases and chitinases”, Journal of Experimental Biology, 206:4393-4412, (2003).
  12. Rost-Roszkowska, M.M., Undrul, A., “Fine structure and differentiation of the midgut epithelium of Allacma fusca (Insecta: Collembola: Symphypleona) ”, Zoological