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Journals / Gazi University Journal of Science / 2018 / Cilt: 31 - Sayı: 4

Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane

Aydın ÖZLÜK
Naznoosh SHOMALI MOGHADDAM
Nursel GÜL
Ebru GÖKALP ÖZKORKMAZ
Pages
1022–1031
Publication Date
2022-10-19
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our examinations revealed that D-galactose mostly exists in A.segetum larvae midgut cell membranes and peritrophic membrane and this means BPA and GSIlectins can be used as insecticidal agents against A. segetum.

📌 3 Key Takeaways

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Objective & Hypothesis:Midgut cell and peritrophic membranes of grain pest Agrotis segetum larvae were examined bylight microscope.
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Key Findings & Data:Results of the studyindicated that lectins BPA (Bauhinia purpurea) and GS-I (Griffonia simplicifolia) stronglystained whereas, PNA (Pea Nut Agglutinin) and UEA-1 (Ulex europaeus) moderately stainedthe tissues.
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Conclusion & Impact:Our examinations revealed that D-galactose mostly exists in A.segetum larvae midgut cell membranes and peritrophic membrane and this means BPA and GSIlectins can be used as insecticidal agents against A. segetum.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Midgut cell and peritrophic membranes of grain pest Agrotis segetum larvae were examined bylight microscope. Six biotinylated lectins were used in the assays by applying histochemicalmethods (Avidin-Biotin-Peroxidase). The aim of this study was to find lectins binding onperitrophic membranes if they were available as insecticidal agents. Results of the studyindicated that lectins BPA (Bauhinia purpurea) and GS-I (Griffonia simplicifolia) stronglystained whereas, PNA (Pea Nut Agglutinin) and UEA-1 (Ulex europaeus) moderately stainedthe tissues. However, WGA (Wheat Germ Agglutinin) and Con-A (Canavalia ensiformis)stained the tissues weakly. The common feature of two binding proteins, BPA and GS-I lectinwas binding to D- galactose. Our examinations revealed that D-galactose mostly exists in A.segetum larvae midgut cell membranes and peritrophic membrane and this means BPA and GSIlectins can be used as insecticidal agents against A. segetum.

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Cite This Article

ÖZLÜK, A., MOGHADDAM, N. S., GÜL, N., ÖZKORKMAZ, E. G. (2022). Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane. Gazi University Journal of Science, Cilt: 31 - Sayı: 4, 1022–1031. https://ajindex.com/articles/521422

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