Dergiler / Acta Veterinaria Eurasia / 2020 / Cilt: 46 - Sayı: 3

Detection of Methicillin-Resistant Staphylococcus aureus in Milk by PCR-Based Phenotyping and Genotyping

Sayfa
120–124
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Strategies required to control antimicrobial resistance should be developed and implemented in the field of veterinary and human medicine

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Staphylococcus aureus is a primary cause of food poisoning and many types of other infections in humans and animals.
📊
Temel Bulgular & Veri:A total of 406 raw milk samples were collected from 6 different dairy farms located in Istanbul at different times and under appropriate conditions.
💡
Sonuç & Katkı:Strategies required to control antimicrobial resistance should be developed and implemented in the field of veterinary and human medicine
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

Staphylococcus aureus is a primary cause of food poisoning and many types of other infections in humans and animals. This study aims to detect methicillin-resistant S. aureus (MRSA) in raw cow milk collected from different farms in Istanbul, using polymerase chain reaction–based phenotyping and genotyping. A total of 406 raw milk samples were collected from 6 different dairy farms located in Istanbul at different times and under appropriate conditions. The samples were transferred to laboratory under cold storage conditions. After S. aureus had been isolated from the samples by phenotypic methods, MRSA strains were detected in isolates by both phenotypic and genotypic methods. Of the 406 milk samples examined, S. aureus strains were detected in 119 (29.31%). From the 119 isolated S. aureus strains, there was merely 1 (0.84%) strain with MRSA in phenotypic and genotypic terms. Strategies required to control antimicrobial resistance should be developed and implemented in the field of veterinary and human medicine

🔖

Bu Makaleye Atıf Yap

ISSA, G., AKSU, H. (2020). Detection of Methicillin-Resistant Staphylococcus aureus in Milk by PCR-Based Phenotyping and Genotyping. Acta Veterinaria Eurasia, Cilt: 46 - Sayı: 3, 120–124. https://ajindex.com/articles/141662

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (8)

  1. Adaleti, R., Nakipoglu, Y., Karahan, Z.C., Tasdemir, C., Kaya, F., 2008. Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus. The Journal of Infection in Developing Countries 2, 46-50. [Crossref] Araj, G.F., Talhouk, R.S., Simaan, C.J., Maasad, M.J., 1999. Discrepancies between mecA PCR and conventional tests used for detection of methicillin resistant Staphylococcus aureus. International Journal of Antimicrobial Agents 11, 47-52. [Crossref] Bilgehan, H. 2000.
  2. Klinik Mikrobiyoloji, Özel Bakteriyoloji ve Bakteri Enfeksiyonları. 10. Baskı. Barış Yayınları Fakülteler Kitapevi, 241-265, İzmir. Clinical and Laboratory Standards Institute (CLSI), 2009. Performance standards for antimicrobial susceptibility testing; Nineteenth Informational Supplement, CLSI document M100-S19, 29, USA. Ergün, Y., Aslantaş, Ö.,, Doğruer, G., Cantekin, Z., 2004. Hatay ilindeki aile tipi süt sığırcılığı işletmelerinde subklinik mastitislerin epidemiyolojisi. Veteriner Bilimleri Dergisi 20, 25-28.
  3. Erol, İ., 2007. Gıda Hijyeni ve Mikrobiyolojisi Ders Kitabı. Pozitif Matbaacılık Ltd.Şti., 135-144,
  4. Ankara.
  5. Huber, H., Koller, S., Giezendanner, N., Stephan R, Zweifel, L., 2010. Prevalence and characteristics of methicillin-resistant Staphylococcus aureus in human in contact with farm animals, in livestock, and in food of animal origin, Switzerland 2009. Euro Surveillance 15, 19542. Jonas, D., Speck, M., Daschner F.D., Grundmann, H., 2002. Rapid PCR-based identification of methicillin-resistant Staphylococcus aureus from screening swabs. Journal of Clinical Microbiology 40, 1821-1823.[Crossref] Juhász-Kaszanyitzky, E., Jánosi, S., Somogyi, P., Dán, A., van der Graaf-van Bloois, L., van Duijkeren, E., Wagenaar, J.A. (2007). MRSA transmission between cows and humans. Emerging infectious diseases, 13(4), 630-632. [Crossref] Kayser, H., Bienz, A., Eckert, J., Lindenmann, J., 1997. Hücre duvarı, tıbbi mikrobiyoloji, immunoloji, bakteriyoloji, mikoloji, viroloji, parazitoloji. In: Küçüker Anğ, M, Tümbay, E, Anğ, Ö, Tıbbi Mikrobiyoloji, Nobel Tip Kitapevleri, 84-87,
  6. İstanbul.
  7. Kuyucuoğlu, Y., Uçar, M., 2001. Afyon bölgesi süt ineklerinde subklinik ve klinik mastitislerin görülme oranları ve etkili antibiyotiklerin tespiti. Veteriner Hekimliği Mikrobioloji Dergisi 1, 19-24.
  8. Kwon, N.H., Park, K.T., Moon, J.S., Jung, W.K., Kim, S.H., Kim, J.M., Hong, S.K., Koo, H.C., Joo, Y.S., Park, Y.H., 2005. Staphylococcal casette chromosome m