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Dergiler / Hittite Journal of Science and Engineering / 2026 / Volume: 12 Issue: 3

Optimization of Solvent Concentration in Phenolic Compound Extraction from Inula helenium L. Leaves Using D-Optimal Mixture Design

Esra Esin(TOKAT GAZİOSMANPAŞA ÜNİVERSİTESİ)0000-0003-0470-0015
Semra Topuz Türker(TOKAT GAZİOSMANPAŞA ÜNİVERSİTESİ)0000-0002-9122-0839
Sayfa
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Yayın Tarihi
2025-09-30
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

These results indicate that I. helenium leaf extracts, with high phenolic content and potent antioxidant activity, represent a promising natural antioxidant source, particularly for applications in the food industry.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:nula helenium L. (Elecampane) leaves are an underutilized source of natural antioxidants due to their rich phenolic content.
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Temel Bulgular & Veri:Various solvent mixtures (ethanol, methanol, and water) were tested, and the optimal composition was determined as 42.40% ethanol, 0.00% methanol, and 57.60% water.
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Sonuç & Katkı:These results indicate that I. helenium leaf extracts, with high phenolic content and potent antioxidant activity, represent a promising natural antioxidant source, particularly for applications in the food industry.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Elecampane · Phenolic compound · D-Optimal mixture design

Abstract

nula helenium L. (Elecampane) leaves are an underutilized source of natural antioxidants due to their rich phenolic content. Although most studies have investigated roots or other aerial parts, limited information is available on phenolic extraction from I. helenium leaves. This study aimed to optimize the solvent concentrations for phenolic compound extraction from I. helenium leaves using D-Optimal Mixture Design and to evaluate the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities of the extracts. Various solvent mixtures (ethanol, methanol, and water) were tested, and the optimal composition was determined as 42.40% ethanol, 0.00% methanol, and 57.60% water. Under these conditions, TPC and TFC were found to be 28.12 mg gallic acid equivalent (GAE)/g dry sample and 27.74 mg quercetin equivalent (QE)/g dry sample, respectively. Antioxidant activities measured by ABTS, DPPH, and FRAP assays were 19.73, 1.42, and 6.10 mg Trolox equivalent (TE)/g dry sample, respectively. These results indicate that I. helenium leaf extracts, with high phenolic content and potent antioxidant activity, represent a promising natural antioxidant source, particularly for applications in the food industry.

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Bu Makaleye Atıf Yap

Esin, E., Türker, S. T. (2025). Optimization of Solvent Concentration in Phenolic Compound Extraction from Inula helenium L. Leaves Using D-Optimal Mixture Design. Hittite Journal of Science and Engineering, Volume: 12 Issue: 3. https://doi.org/10.17350/HJSE19030000362

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