Dergiler / Anadolu Orman Araştırmaları Dergisi / 2026 / Sayı: 13

Pinus sylvestris L. ibrelerinin uçucu bileşik ve fenolik profillerinde yaşa bağlı değişimler

Age-related variation in the volatile compounds and phenolic profile of Pinus sylvestris L. needles

Fatma Merve Nacakcı(Isparta Uygulamalı Bilimler Üniversitesi Orman fakültesi)0000-0002-6908-2046
Nazlı Öğüt(ISPARTA UYGULAMALI BİLİMLER ÜNİVERSİTESİ, ORMAN FAKÜLTESİ, ORMAN MÜHENDİSLİĞİ BÖLÜMÜ)0009-0003-8375-3808
Sayfa
—
Yayın Tarihi
2026-10-12
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bulgular Analizler sonucunda toplam 81 uçucu bileşik tespit edilmiştir. α-pinene (%18,79), caryophyllene (%11,10), germacrene D (%10,94) ve β-pinene (%7,47) başlıca bileşenler olarak belirlenmiştir.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Giriş ve Hedefler Sarıçam (Pinus sylvestris L.), geniş yayılış alanı ve zengin uçucu bileşik içeriği nedeniyle ekolojik ve ekonomik açıdan önemli bir türdür.
📊
Temel Bulgular & Veri:Bu çalışmada, farklı yaş gruplarındaki sarıçam bireylerinin iğne yapraklarında bulunan uçucu bileşiklerin belirlenmesi ve yaşın bu bileşikler üzerindeki etkisinin ortaya konulması amaçlanmıştır.
💡
Sonuç & Katkı:Bulgular Analizler sonucunda toplam 81 uçucu bileşik tespit edilmiştir. α-pinene (%18,79), caryophyllene (%11,10), germacrene D (%10,94) ve β-pinene (%7,47) başlıca bileşenler olarak belirlenmiştir.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Yaşa bağlı değişim · uçucu bileşen · GC-MS

Özet

Giriş ve Hedefler Sarıçam (Pinus sylvestris L.), geniş yayılış alanı ve zengin uçucu bileşik içeriği nedeniyle ekolojik ve ekonomik açıdan önemli bir türdür. Bu çalışmada, farklı yaş gruplarındaki sarıçam bireylerinin iğne yapraklarında bulunan uçucu bileşiklerin belirlenmesi ve yaşın bu bileşikler üzerindeki etkisinin ortaya konulması amaçlanmıştır. Bulgular Analizler sonucunda toplam 81 uçucu bileşik tespit edilmiştir. α-pinene (%18,79), caryophyllene (%11,10), germacrene D (%10,94) ve β-pinene (%7,47) başlıca bileşenler olarak belirlenmiştir. Tüm yaş gruplarında monoterpen hidrokarbonlar baskın bileşik sınıfını oluşturmuştur. Ayrıca, bazı seskiterpen bileşiklerin yaşla birlikte artış eğilimi gösterdiği belirlenmiştir. Kateşin en yüksek miktarda bulunan fenolik bileşen olurken, epikateşin ve p-kumarik asit yalnızca 25 yaş grubunda belirlenmiştir. PCA sonuçları, yaş gruplarının uçucu bileşik profilleri bakımından birbirinden ayrıldığını ve yaşın kimyasal kompozisyon üzerinde etkili bir faktör olduğunu ortaya koymuştur. Sonuçlar Elde edilen bulgular, sarıçam iğnelerinin uçucu bileşik profilinin yaşa bağlı olarak değiştiğini göstermektedir. Sonuçlar, türün kimyasal özelliklerinin daha iyi anlaşılmasına katkı sağlamaktadır. Ayrıca farmakolojik, aromatik ve endüstriyel amaçlarla değerlendirilecek uçucu bileşiklerin elde edilmesinde uygun yaş grubunun belirlenmesine yönelik çalışmalara temel oluşturabilecek niteliktedir.

Abstract

Background and aims Scots pine (Pinus sylvestris L.) is an ecologically and economically important species due to its wide distribution and rich content of volatile compounds. The aim of this study was to identify the volatile compounds present in the needles of Scots pine individuals of different age groups and to determine the effect of age on these compounds. Methods The study material consisted of needle samples collected from 5-, 25-, and 70-year-old Scots pine trees growing in Gölcük Nature Park. The GC-MS method was used to identify volatile compounds, and the RP-HPLC method was used to identify phenolic compounds; differences among age groups were evaluated using statistical analyses and Principal Component Analysis (PCA). Results The analyses identified a total of 81 volatile compounds. α-pinene (18.79%), caryophyllene (11.10%), germacrene D (10.94%), and β-pinene (7.47%) were identified as the major compounds. Monoterpene hydrocarbons constituted the major compound class across all age groups. Additionally, it was determined that the levels of some sesquiterpene compounds tended to increase with age. While catechin was the phenolic compound present in the highest amount, epicatechin and p-coumaric acid were detected only in the 25-year-old age group. PCA results revealed that the age groups were distinguishable from one another in terms of their volatile compound profiles and that age is a significant factor influencing chemical composition. Conclusions The findings indicate that the volatile compound profile of Scots pine needles changes with age. These results contribute to a better understanding of the species’ chemical properties. Furthermore, they provide a basis for studies aimed at determining the appropriate age group for the extraction of volatile compounds for pharmacological, aromatic, and industrial applications.

🔖

Bu Makaleye Atıf Yap

Nacakcı, F. M., Öğüt, N. (2026). Pinus sylvestris L. ibrelerinin uçucu bileşik ve fenolik profillerinde yaşa bağlı değişimler. Anadolu Orman Araştırmaları Dergisi, Sayı: 13. https://doi.org/10.53516/ajfr.1976626

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (58)

  1. Aggarwal, S., Thareja, S., Verma, A., Bhardwaj, T.R., Kumar, M., 2010. An overview on 5α-reductase inhibitors. Steroids, 75(2),109-153. https://doi.org/10.1016/j.steroids.2009.10.005.
  2. Boratynski, A., 1991. Range of natural distribution. In: Giertych, M., Matyas, C., eds. Genetics of Scots pine. Developments in plant genetics and breeding, 19-30.
  3. Borges, R.S., Ortiz, B.L.S., Pereira, A.C.M., Keita, H., Carvalho, J.C.T., 2019. Rosmarinus officinalis essential oil: a review of its phytochemistry, anti-inflammatory activity, and mechanisms of action involved. J. Ethnopharmacol., 229, 29-45. https://doi.org/10.1016/j.jep.2018.09.038.
  4. Brichta, J., Vacek, S., Vacek, Z., Cukor, J., Mikeska, M., Bílek, L., Brabec, P., 2023. Importance and potential of Scots pine (Pinus sylvestris L.) in 21st century. Central European Forestry Journal, 69(1), 3-20. https://doi.org/10.2478/forj-2022-0020.
  5. Charrier, G., Ngao, J., Saudreau, M., Améglio, T., 2015. Effects of environmental factors and management practices on microclimate, winter physiology, and frost resistance in trees. Frontiers in plant science, 6, 259. https://doi.org/10.3389/fpls.2015.00259.
  6. Dziedziński, M., Kobus-Cisowska, J., Stachowiak, B., 2021. Pinus species as prospective reserves of bioactive compounds with potential use in functional food current state of knowledge. Plants, 10(7), 1306. https://doi.org/10.3390/plants10071306.
  7. Eckenwalder, J. E., 2009. Conifers of the World. The complete referens. Timber Press, Portland, London.
  8. El-Sawi, S.A., Motawae, H.M., Ali, A.M., 2007. Chemical composition, cytotoxic activity and antimicrobial activity of essential oils of leaves and berries of Juniperus phoenicea L. grown in Egypt. African Journal of Traditional, Complementary and Alternative Medicines, 4(4), 417-426.
  9. Farjon, A., 2010. A Handbook of the World's Conifers (2, 1). Brill academic publishers, USA.
  10. Ghahremani, A.; Ghasemi Pirbalouti, A.; Mozafari, H.; Habibi, D.; Sani, B., 2020. Phytochemical and morpho-physiological traits of mullein as a new medicinal crop under different planting pattern and soil moisture conditions. Ind. Crop. Prod., 145, 111976. https://doi.org/10.1016/j.indcrop.2019.111976.
  11. Gül, A., Özgüner, H., Akten, M., Küçük, V., 2004. Gölcük gölü ve çevresi peyzaj planlama ve tasarım projesi. SDÜ, Bilimsel Araştırma Yönetim Birimi Alt Yapı Projesi, (2002/2).
  12. Gülsoy, S., Akdemir, D., Özdemir, S., Aydın, S., Dalgıç, L., 2014. Göller yöresi boylu ardıç (Juniperus excelsa Bieb.) sahalarında çevresel faktörlerin kozalak fiziksel özellikleri üzerine etkisi. II. Ulusal Akdeniz Orman ve Çevre Sempozyumu: Akdeniz Ormanlarının Geleceği - Sürdürülebilir Toplum ve Çevre, Isparta, 750- 762.
  13. Gülsoy, S., Özkan, G., Özkan, K., Genç, M., 2013. Effects of ecological factors on some physical and physicochemical fruit characteristics of turpentine tree (Pistacia terebinthus L. subsp. palaestina (Boiss.) Engler). SDU Faculty of Forestry Journal, 14, 15-23. https://doi.org/10.18182/tjf.99109.
  14. Gülsoy, S., Turhan, U. U., Özkan, G., 2017. Relationships between essential oil yield and physical properties of cones in juniper species (Juniperus excelsa Bieb. and Juniperus foetidissima Willd.). Turkish Journal of Forestry,18(3), 219-225. https://doi.org/10.18182/tjf.305958.
  15. Hajdari, A., Mustafa, B., Nebija, D., Selimi, H., Veselaj, Z., Breznica, P., Quave, C.L., Novak, J., 2016. Essential oil composition of Pinus peuce griseb. needles and twigs from two national parks of Kosovo. The Scientific World Journal, 2016(1), 5393079. https://doi.org/10.1155/2016/5393079.
  16. Hojjati, F., Zarre, S., Assadi, M., 2009. Isoenzyme diversity and cryptic speciation in Juniperus excelsa (Cupressaceae) complex in Iran. Biochemical Systematics and Ecology, 37(3), 193-200. https://doi.org/10.1016/j.bse.2009.03.002.
  17. Judzentiene, A., Kupcinskiene, E., 2008. Chemical composition on essential oils from needles of Pinus sylvestris L. grown in northern Lithuania. Journal of Essential Oil Research, 20(1), 26-29. https://doi.org/10.1080/10412905.2008.9699413.
  18. Kännaste, A., Copolovici, L., Semiz, G., Borg-Karlson, A. K., Niinemets, Ü., 2026. Broad-scale environmental drivers of needle terpenoid diversity and chemotype distribution in Pinus sylvestris: importance of considering chiral terpene isomers. New Forests, 57(4), 37. https://doi.org/10.1007/s11056-026-10179-0.
  19. Karan, T., Usta, N. C., 2024. Pinus sylvestris var. hamata Uçucu Yağlarının Fitokimyasal Bileşimi, Antioksidan ve Antimikrobiyal Aktiviteleri. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 7(1), 177-189. https://doi.org/10.47495/okufbed.1302045.
  20. Khalil, A., Tazeddinova, D., 2020. The upshot of polyphenolic compounds on immunity amid COVID-19 pandemic and other emerging communicable diseases: An appraisal. Natural Products and Bioprospecting, 10(6), 411. https://doi.org/10.1007/s13659-020-00271-z.
  21. Kim, J. W., Im, S., Jeong, H. R., Jung, Y. S., Lee, I., Kim, K. J., et al., 2018. Neuroprotective effects of Korean red pine (Pinus densiflora) bark extract and its phenolics. J. Microbiol. Biotechnol. 28 (5), 679–687. https://doi.org/10.4014/jmb.1801.01053.
  22. Koukos, P.K., Papadopoulou, K.I., Patiaka, D.T., Papagiannopoulos, A.D., 2000. Chemical composition of essential oils from needles and twigs of Balkan pine (Pinus peuce Grisebach) grown in Northern Greece. J. Agric. Food Chem., 48, 1266-1268. https://doi.org/10.1021/jf991012a.
  23. Kubeczka, K. H., Schultze, W., 1987. Biology and chemistry of conifer oils. Flavour and fragrance journal, 2(4), 137-148. https://doi.org/10.1002/ffj.2730020402.
  24. Lau, B. H., 1998. Rational Phytotherapy: A Physicians' Guide to Herbal Medicine. Ann Intern Med.1998; 128:880. https://doi.org/10.7326/0003-4819-128-10-199805150-00036. Lawlees J., 1992. The encyclopaedia of essential oils. Element Books Limited: Boston. 97-98.
  25. Lawrence, B. M., 2003. Progress in essential oils. Perfumer and Flavorist, 28(1), 60-81.
  26. Lê, S., Josse, J., & Husson, F., 2008. FactoMineR: an R package for multivariate analysis. Journal of statistical software, 25, 1-18. https://doi.org/10.18637/jss.v025.i01.
  27. Lebedev, A. V., 2023. Changes in the growth of Scots pine (Pinus sylvestris L.) stands in an urban environment in European Russia since 1862. Journal of Forestry Research, 34(5), 1279-1287. https://doi.org/10.1007/s11676-022-01569-z.
  28. Leung, A. Y., Foster, S., 1996. Encyclopedia of common natural ingredients used in food, drugs, and cosmetics. 2nd Edition, John Wiley and Sons, Inc., New York.
  29. Luna-Guevara, M. L., Luna-Guevara, J. J., Hernández-Carranza, P., Ruíz-Espinosa, H., Ochoa-Velasco, C. E., 2018. Phenolic compounds: A good choice against chronic degenerative diseases. Studies in natural products chemistry, 59, 79-108. https://doi.org/10.1016/B978-0-444-64179-3.00003-7.
  30. Matías, L., Jump, A. S., 2012. Interactions between growth, demography and biotic interactions in determining species range limits in a warming world: the case of Pinus sylvestris. Forest Ecology and Management, 282, 10-22. https://doi.org/10.1016/j.foreco.2012.06.053.
  31. Mirković, S., Tadić, V., Milenković, M. T., Ušjak, D., Racić, G., Bojović, D., Žugić, A., 2024. Antimicrobial activities of essential oils of different Pinus species from Bosnia and Herzegovina. Pharmaceutics, 16(10), 1331. https://doi.org/10.3390/pharmaceutics16101331.
  32. Nacakci, F. M., Gülcü, S., 2025. Comparison of Scotch pine (Pinus sylvestris L.) origins in terms of photosynthetic gas exchange and chemical properties. BioResources, 20(2), 4681-4700. https://doi.org/10.15376/biores.20.2.4681-4700.
  33. Nacakcı, F.M., 2025. Physical and chemical properties of Crataegus monogyna Jacq. var. monogyna fruits growing in Isparta/Türkiye. Kastamonu University Journal of Forestry Faculty, 25(3), 294-305. https://doi.org/10.17475/kastorman.1845217.
  34. Naeem I, Taskeen A, Mubeen H, Maimoona A., 2010. Characterization of flavonoids present in barks and needles of Pinus wallichiana and Pinus roxburghii. Asia Journal of Chemistry, 22, 41-44.
  35. Naydenov, K. D., Tremblay, F. M., Alexandrov, A., Fenton, N. J., 2005. Structure of Pinus sylvestris L. populations in Bulgaria revealed by chloroplast microsatellites and terpenes analysis: provenance tests. Biochemical systematics and ecology, 33(12), 1226-1245. https://doi.org/10.1016/j.bse.2005.07.011.
  36. Njoku, I. S., Rahman, N. U., Khan, A. M., Otunomo, I., Asekun, O. T., Familoni, O. B., Ngozi, C., 2022. Chemical composition, antioxidant, and antibacterial activity of the essential oil from the leaves of Pinus sylvestris. The Pacific Journal of Science and Technology, 23(1), 85-93.
  37. Öğüt, N., Karagöz, S. G., Ulusan, M. D., 2026. Juniperus turbinata Guss. cone and leaf essential oil compositions with morphological characteristics and altitude interaction. Journal of Anatolian Environmental and Animal Sciences, 11, 1-14. https://doi.org/10.35229/jaes.1894153.
  38. Okoń, S., Wieruszewski, M., Dynowska, J., Ankudo-Jankowska, A., Adamowicz, K., 2024. Cost of regeneration of scots pine (pinus sylvestris l.) crops in national forests. Forests, 15(7), 1218. https://doi.org/10.3390/f15071218.
  39. Osmanoğlu, T., Özdemir, İ., 2014. Isparta-Gölcük Tabiat Parkı ormanında meşcere yapısı ile kuş türü zenginliği arasındaki ilişkiler. Biological Diversity and Conservation, 7(3), 78-86.
  40. Pachura-Hanusek, N., Lewandowska, K., Burek, A., Szumny, A., Tabiś, A., Banaszkiewicz, S., Kupczyński, R., 2026. Chemical characterization and rumen-modulating effects of pinus sylvestris essential oil: in vitro and in vivo study. Molecules, 31(10), 1769. https://doi.org/10.3390/molecules31101769m.
  41. R Core Team., 2026. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://doi.org/10.32614/R.manuals.
  42. Ramos, P. A., Pereira, C., Gomes, A. P., Neto, R. T., Almeida, A., Santos, S. A., et al., 2022. Chemical characterisation, antioxidant and antibacterial activities of Pinus pinaster ait. and Pinus pinea L. bark polar extracts: prospecting forestry by-products as renewable sources of bioactive compounds. Appl. Sci. 12 (2), 784. https://doi.org/10.3390/app12020784.
  43. Ren, T., Zheng, P., Zhang, K., Liao, J., Xiong, F., Shen, Q., Ma, Y., Fang, W., Zhu, X., 2021. Effects of GABA on the polyphenol accumulation and antioxidant activities in tea plants (Camellia sinensis L.) under heat-stress conditions. Plant Physiol. Biochem., 159, 363-371. https://doi.org/10.1016/j.plaphy.2021.01.003.
  44. Rinne, J., Hakola, H., Laurila, T., Rannik, Ü., 2000. Canopy scale monoterpene emissions of Pinus sylvestris dominated forests. Atmospheric Environment, 34(7), 1099-1107. https://doi.org/10.1016/S1352-2310(99)00335-0.
  45. Saleem, A., Kivelä, H., Pihlaja, K., 2003. Antioxidant activity of pine bark constituents. Z Naturforsch C 58 (5-6), 351–354.
  46. Slimestad, R., 2003. Flavonoids in buds and young needles of Picea, Pinus and Abies. Biochemical Systematics and Ecology, 31, 1247-1255. https://doi.org/10.1016/S0305-1978(03)00018-8.
  47. Szmechtyk, T., Małecka, M., 2024. Phytochemicals from bark extracts and their applicability in the synthesis of thermosetting polymers: an overview. Materials 17 (9), 2123. https://doi.org/10.3390/ma17092123.
  48. Tekeş, A., Karagöz, S. G., Demir, O., Oğuzoğlu, Ş., Öğüt, N., Arslan, M., Gülsoy, S., (2024). Elevation variations in volatile components of St. John’s Wort (Hypericum perforatum L.). https://doi.org/10.18182/tjf.1487768.
  49. TSMS, 2026. Turkish State Meteorological Service, Ankara. https://www.mgm.gov.tr/eng/forecast-cities.aspx, Accesed: 12.03.2026.
  50. Tümen, İ., Reunanen, M., 2010. A comparative study on turpentine oils of oleoresins of Pinus sylvestris L. from three districts of Denizli. Records of Natural Products, 4(4), 224.
  51. Ustun, O., Sezik, E., Kurkcuoglu, M., Baser, K.H.C., 2006. Study of the essential oil composition of Pinus sylvestris from Turkey. Chem. Nat. Compd., 42, 26-31. https://doi.org/10.1007/s10600-006-0029-2.
  52. Venskutonis, P. R., Vyskupaityte, K., Plausinaitis, R., 2000. Composition of essential oils of Pinus sylvestris L. from different locations of Lithuania. Journal of Essential Oil Research, 12(5), 559-565. https://doi.org/10.1080/10412905.2000.9712159.
  53. Viehweger, K., 2014. How plants cope with heavy metals. Botanical Studies, 55(1), 35.
  54. Wilkinson, L., 2011. ggplot2: elegant graphics for data analysis by WICKHAM, H. 678-679. https://doi.org/10.1111/j.1541-0420.2011.01616.x.
  55. Yaltırık, F., 1988. Dendroloji Ders Kitabı. Istanbul University, Faculty of forestry publications, İstanbul.
  56. Yavuz, M., 2010. Gölcük Tabiat Parkı liken florası ve Isparta hava kirliliğinin likenlerle derecelendirilmesi. Master’s thesis, Marmara University, Turkey.
  57. Yesil-Celiktas, O., Otto, F., Gruener, S., Parlar, H., 2009. Determination of extractability of pine bark using supercritical CO2 extraction and different solvents: optimization and prediction. J. Agric. Food Chem. 57 (2), 341–347. https://doi.org/10.1021/jf8026414.
  58. Yılmazer, M., Karagöz, S. G., Ozkan, G., Karacabey, E., 2016. Aroma transition from rosemary leaves during aromatization of olive oil. Journal of food and drug analysis, 24(2), 299-304. https://doi.org/10.1016/j.jfda.2015.11.002.