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Dergiler / Acta Medica Nicomedia / 2026 / Cilt: 8 Sayı: 2

ROSMARİNİK ASİTİN İNSAN KOLON KANSERİNDE OTOFAJİK ETKİSİ

AUTOPHAGIC EFFECT OF ROSMANIRIC ACID ON HUMAN COLON CANCER

İlkay Çorumluoğlu(ANKARA YILDIRIM BEYAZIT ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-5125-302X
Ebru Alimogullari(ANKARA YILDIRIM BEYAZIT ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-9557-3631
Sayfa
126–132
Yayın Tarihi
2025-06-30
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bulgular: LPS hasarı oluşturulan grupta kontrol grubuna göre otofajik protein ifadesi yüksek bulunurken, LPS+RA verilen grupta diğer gruplar ile karşılaştırıldığında istatistiksel olarak anlamlı bulundu (p<0,05).

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Amaç: Kolon kanseri en agresif kanser türüdür ve tedavilerin yetersiz olmasından kaynaklı ileri aşamalarda kötü prognoz ile karakterizedir.
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Temel Bulgular & Veri:Rosmarinik asit kolon, pankreas, prostat, karaciğer ve multipl miyelom dahil olmak üzere çeşitli kanserlerdeki lezyonlara karşı etkili olduğu kanıtlanmıştır.
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Sonuç & Katkı:Bulgular: LPS hasarı oluşturulan grupta kontrol grubuna göre otofajik protein ifadesi yüksek bulunurken, LPS+RA verilen grupta diğer gruplar ile karşılaştırıldığında istatistiksel olarak anlamlı bulundu (p<0,05).
🔬Tür:Deneysel Tarla Denemesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Caco-2 · Beclin-1 · p62

Özet

Amaç: Kolon kanseri en agresif kanser türüdür ve tedavilerin yetersiz olmasından kaynaklı ileri aşamalarda kötü prognoz ile karakterizedir. Rosmarinik asit kolon, pankreas, prostat, karaciğer ve multipl miyelom dahil olmak üzere çeşitli kanserlerdeki lezyonlara karşı etkili olduğu kanıtlanmıştır. Yöntem: Çalışmamızda lipopolisakkarit (LPS) ile oluşturulan hücresel hasar ve otofaji üzerine rozmanirik asitin kolon kanseri hücrelerine etkisini araştırmayı amaçladık. Çalışmamızda Caco-2 (HTB-37) insan kolon kanseri hücre hattı kullanıldı. Kontrol, LPS, RA ve LPS+RA olmak üzere 4 grup dizayn edildi. Caco-2 için 200 µg/mL RA dozu ve LPS dozu 10 μg/ml kullanılmıştır. Caco-2 hücre hatları kültüre edildikten sonra Beclin-1, p62 ve LC3 proteinlerinin hücresel lokalizasyonlarını belirlemek için immünohistokimyasal analizler gerçekleştirildi. Otofajik yolaktaki bu proteinlerin ifadesini belirlemek için western blot analizleri yapıldı. Bulgular: LPS hasarı oluşturulan grupta kontrol grubuna göre otofajik protein ifadesi yüksek bulunurken, LPS+RA verilen grupta diğer gruplar ile karşılaştırıldığında istatistiksel olarak anlamlı bulundu (p<0,05). Sonuç: Bu çalışma ile rosmarinik asitin kolon kanseri hücrelerinde otofaji yolağında önemli proteinlerin degredasyonuna karşı klinik etkilerinin tespit edilebilmesi için daha kapsamlı çalışmaların yapılması gerektiğini önermekteyiz.

Abstract

Objective: Colon cancer is the most aggressive type of cancer and is characterized by a inefficient prognosis in advanced stages due to inadequate treatment. Rosmarinic acid has been shown to be effective against lesions in various cancers, including colon, pancreatic, prostate, liver and multiple myeloma. In our study, we aimed to investigate the effect of rosmarinic acid on lipopolysaccharide (LPS)-induced cell damage and autophagy in colorectal cancer cells. Methods: The human colon cancer cell line Caco-2 (HTB-37) was used in our study. 4 groups were formed: Control, LPS, RA and LPS+RA. For Caco-2, an RA dose of 200 µg/ml and an LPS dose of 10 µg/ml were used. After the Caco-2 cell lines were cultured, immunohistochemical analyzes were performed to determine the cellular localizations of Beclin-1, p62 and LC3 proteins. Western blot analyzes were performed to determine the expression of these proteins in the autophagic pathway. Results: While autophagic protein expression was higher in the LPS-damaged group compared to the control group, it was statistically significant in the LPS+RA group compared to the other groups (p<0,05). Conclusion: In conclusion, with this study we suggest that more comprehensive studies should be conducted to determine the clinical effect of rosmarinic acid against the degradation of key proteins in the autophagy pathway in colorectal cancer cells.

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Bu Makaleye Atıf Yap

Çorumluoğlu, İ., Alimogullari, E. (2025). ROSMARİNİK ASİTİN İNSAN KOLON KANSERİNDE OTOFAJİK ETKİSİ. Acta Medica Nicomedia, Cilt: 8 Sayı: 2, 126–132. https://doi.org/10.53446/actamednicomedia.1589884

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