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Dergiler / Experimed / 2026 / Volume: 15 Issue: 1

Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia

İlkay Pişkin(YILDIRIM BEYAZIT UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY)0000-0002-5125-302X
Tuba Özdemir Sancı(YILDIRIM BEYAZIT UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY)0000-0002-9468-4719
Yasin Köksal(Ministry of Health Ankara Bilkent City Hospital, Stem Cell Processing Laboratory, Department of Pediatric Hematology Oncology)0000-0003-0797-9568
Hüsniye Neşe Yaralı(Ministry of Health Ankara Bilkent City Hospital, Department of Pediatric Hematology)0000-0001-5488-2385
Fatma Karaca Kara(Department of Medical Biochemistry, Ministry of Health Ankara Kecioren Training and Research Hospital)0000-0002-4379-3085
Bahattin Tunç(Ankara Child Health and Diseases Hematology Oncology Training and Research Hospital)0000-0003-3800-0519
Meltem Özgüner(YILDIRIM BEYAZIT UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY)0000-0003-0364-5907
Sayfa
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Yayın Tarihi
2025-04-16
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

This interaction serves as a marker for understanding the immunological effects of AML.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: Pediatric acute myeloid leukemia (AML) is a common form of pediatric leukemia and is characterized by the accumulation of abnormal white blood cells, called blasts, in the bone marrow (BM).
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Temel Bulgular & Veri:Results: Mononucleated cells from peripheral blood of AML patients and donors and T-cell activation markers (CD3+CD69+, CD4+C25+, CD3+HLA-DR+) were measured by flow cytometry (𝜒2=2.184; p=0.823).
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Sonuç & Katkı:This interaction serves as a marker for understanding the immunological effects of AML.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Pediatric AML · BM-MSCs · PHA

Abstract

Objective: Pediatric acute myeloid leukemia (AML) is a common form of pediatric leukemia and is characterized by the accumulation of abnormal white blood cells, called blasts, in the bone marrow (BM). The aim of this study was to understand the BM microenvironment by studying the biological and immunological properties of BM-derived mesenchymal stromal cells (MSCs) and mononuclear cells (MNCs) to elucidate the potential role of phytohaemag glutinin in cell viability. Materials and Methods: BM and peripheral blood samples were obtained from seven pediatric AML patients and seven donors. BM-MSCs and MNCs were isolated and characterized. Population doubling (PD) values, adipogenic and osteogenic differentiation capacity, cell viability, phytohemagglutinin (PHA) assay, and flow cytometry were performed. Results: Mononucleated cells from peripheral blood of AML patients and donors and T-cell activation markers (CD3+CD69+, CD4+C25+, CD3+HLA-DR+) were measured by flow cytometry (𝜒2=2.184; p=0.823). BM-MSCs were co-cultured with MNCs, and PD values for AML patients were similar to those of donors (z=1.074; p=0.394). It was statistically significant when healthy MNC and healthy MNC PHA(+) groups were compared (p=0.015). When healthy MNC PHA(+) and healthy MSC+AML MNC PHA(+) groups were compared, it was found to be statistically significant (p=0.014). Conclusion: This interaction is also not unidirectional. This interaction serves as a marker for understanding the immunological effects of AML.

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Bu Makaleye Atıf Yap

Pişkin, İ., Sancı, T. Ö., Köksal, Y., Yaralı, H. N., Kara, F. K., Tunç, B., Özgüner, M. (2025). Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia. Experimed, Volume: 15 Issue: 1. https://doi.org/10.26650/experimed.1558871

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