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Dergiler / Experimed / 2026 / Volume: 16 Issue: 2

Integrated Analysis of OX40/OX40L Genetic Variants and Circulating Soluble Immune Checkpoint Markers in Laryngeal Cancer Recurrence

Maral Farzam(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE, DEPARTMENT OF MOLECULAR MEDICINE)0000-0003-0397-6992
Dilara Sönmez(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE, DEPARTMENT OF MOLECULAR MEDICINE)0000-0002-4962-5330
Ülgen Sever(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE, THE DEPARTMENT OF IMMUNOLOGY)0000-0002-9754-0446
Cem Horozoğlu(HALIC UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF MEDICAL BIOCHEMISTRY)0000-0001-8998-2028
Saime Sürmen(UNIVERSITY OF HEALTH SCIENCES, INSTITUTE OF HEALTH SCIENCES, DEPARTMENT OF MOLECULAR MEDICINE)0000-0002-7748-0757
Onur Dülger(ONDOKUZ MAYIS UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF PRE-CLINICAL SCIENCES, DEPARTMENT OF PATHOLOGY)0000-0002-1155-7790
Vahid Chaleshi(Shahid Beheshti University of Medical Sciences, Research Institute for Gastroenterology and Liver Diseases, Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center)0000-0001-5355-8866
Mehmet Özkır(YILDIZ TECHNICAL UNIVERSITY, FACULTY OF ARTS AND SCIENCES, DEPARTMENT OF MOLECULAR BIOLOGY AND GENETICS, MOLECULAR BIOLOGY AND GENETICS PR.)0000-0003-4687-710X
Ayşegül Verim(Haydarpasa Numune Education and Research Hospital, Department of Otorhinolaryngology/Head and Neck Surgery,)0000-0002-6649-0837
Yusufhan Süoğlu(ISTANBUL UNIVERSITY, İSTANBUL FACULTY OF MEDICINE, DEPARTMENT OF SURGICAL MEDICAL SCIENCES, DEPARTMENT OF OTORHİNOLARYNGOLOGY)0000-0002-4547-5320
İlhan Yaylım(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE, DEPARTMENT OF MOLECULAR MEDICINE)0000-0003-2615-0202
Sayfa
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Yayın Tarihi
2026-08-28
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Given the cross-sectional and exploratory nature of the study, these findings should be interpreted with caution and require validation in larger prospective cohorts.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: Laryngeal cancer is an aggressive malignancy in which immune regulatory mechanisms play a critical role in tumor progression and recurrence.
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Temel Bulgular & Veri:The OX40/OX40L axis, a member of the tumor necrosis factor superfamily, has been implicated in immune modulation; however, its genetic and soluble profiles in laryngeal cancer remain insufficiently characterized.
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Sonuç & Katkı:Given the cross-sectional and exploratory nature of the study, these findings should be interpreted with caution and require validation in larger prospective cohorts.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Laryngeal cancer · OX40 · OX40L

Abstract

Objective: Laryngeal cancer is an aggressive malignancy in which immune regulatory mechanisms play a critical role in tumor progression and recurrence. The OX40/OX40L axis, a member of the tumor necrosis factor superfamily, has been implicated in immune modulation; however, its genetic and soluble profiles in laryngeal cancer remain insufficiently characterized. Beyond cancer susceptibility, the clinical relevance of OX40/OX40L alterations in recurrence biology remains poorly understood. Materials and Methods: In this study, we investigated the distribution of OX40 rs17568 and OX40L rs1234313 polymorphisms and evaluated their association with serum soluble OX40 (sOX40) and OX40L (sOX40L) levels in 80 patients with laryngeal cancer and 111 healthy controls. Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), and soluble protein levels were quantified by enzyme-linked immunosorbent assay (ELISA). Results: No significant differences in serum sOX40 or sOX40L levels were observed between patients and controls. While OX40 rs17568 genotype distributions were comparable between groups, a significant difference was detected for OX40L rs1234313, with homozygous genotypes (AA and GG) being more frequent in laryngeal cancer patients (p=0.007). Although genotype distributions were not associated with clinicopathological parameters, circulating sOX40 levels were significantly higher in patients with disease recurrence than in those without recurrence (p=0.008). Conclusion: The findings suggest that alterations in the OX40/OX40L axis may be associated with immune dysregulation during disease progression rather than cancer initiation in laryngeal cancer. Particularly, elevated circulating sOX40 levels were associated with disease recurrence. Given the cross-sectional and exploratory nature of the study, these findings should be interpreted with caution and require validation in larger prospective cohorts.

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Bu Makaleye Atıf Yap

Farzam, M., Sönmez, D., Sever, Ü., Horozoğlu, C., Sürmen, S., Dülger, O., Chaleshi, V., Özkır, M., Verim, A., Süoğlu, Y., Yaylım, İ. (2026). Integrated Analysis of OX40/OX40L Genetic Variants and Circulating Soluble Immune Checkpoint Markers in Laryngeal Cancer Recurrence. Experimed, Volume: 16 Issue: 2. https://doi.org/10.26650/experimed.1964715

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