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Dergiler / Meandros Medical And Dental Journal / 2025 / Volume: 26 Issue: 4

Yumurtalık kanserinde borik asit ve kalsiyum fruktoboratın etkisi

Boric acid and Calcium fructoborate effect in ovarian cancer

Ülkü Güler(HACETTEPE ÜNİVERSİTESİ, KİMYA FAKÜLTESİ)0000-0001-6914-9362
Seda Sabah Özcan(CELÂL BAYAR ÜNİVERSİTESİ)0000-0002-8340-331X
Rehime Yapar(PAMUKKALE UNIVERSITY)0000-0002-3117-5431
Bora Onat(3Department of Computational Biomedicine, Cedars-Sinai Medical Center)0000-0003-0388-7201
Mehmet Korkmaz(CELAL BAYAR UNIVERSITY)0000-0003-1058-5586
Bekir Salih(HACETTEPE UNIVERSITY, FACULTY OF CHEMISTRY)0000-0002-8542-6531
Sayfa
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Yayın Tarihi
2025-12-25
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Sonuç olarak, Kalsiyum fruktoborat (CFB) bazı örneklerde apoptozla ilişkili proteinlerin ifadesini belirgin şekilde azaltarak güçlü bir biyolojik yanıt ortaya çıkarmış olabilir ve DNA onarımıyla ilişkili proteinlerin ifadesinde borik asitten daha belirgin bir değişikliğe neden olduğu görülmektedir.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Borik Asit (BA) ve Kalsiyum Fruktoborat'ın (CaF) apoptotik yolaklar ve DNA hasar/onarım yolakları açısından hücrelerin proteomik profili üzerindeki etkilerini araştırmak için A2780 yumurtalık kanseri hücre hattı kullanılmıştır.
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Temel Bulgular & Veri:Her örnekten peptitler aşağıdan yukarıya proteomik analiz için elde edilmiş ve LC-MS/MS (DionexUltimate 3000 RSLCnano system/Easy Spray/Q-Exactive Plus, Thermo Scientific, ABD) üzerinde çalıştırılmıştır.
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Sonuç & Katkı:Sonuç olarak, Kalsiyum fruktoborat (CFB) bazı örneklerde apoptozla ilişkili proteinlerin ifadesini belirgin şekilde azaltarak güçlü bir biyolojik yanıt ortaya çıkarmış olabilir ve DNA onarımıyla ilişkili proteinlerin ifadesinde borik asitten daha belirgin bir değişikliğe neden olduğu görülmektedir.
🔬Tür:Laboratuvar & Saha Deneyi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:proteomic · ovarian cancer · LC-MS/MS

Özet

Borik Asit (BA) ve Kalsiyum Fruktoborat'ın (CaF) apoptotik yolaklar ve DNA hasar/onarım yolakları açısından hücrelerin proteomik profili üzerindeki etkilerini araştırmak için A2780 yumurtalık kanseri hücre hattı kullanılmıştır. Hücre canlılığı ve toksisitesi MTT yöntemi ile belirlenmiştir. Hücreler MTT sonuçlarına göre BA ve CaF ile muamele edilmiş ve protein izolasyonu için toplanmıştır. Protein izolasyonu hücre hattından gerçekleştirilmiştir. Her örnekten peptitler aşağıdan yukarıya proteomik analiz için elde edilmiş ve LC-MS/MS (DionexUltimate 3000 RSLCnano system/Easy Spray/Q-Exactive Plus, Thermo Scientific, ABD) üzerinde çalıştırılmıştır. LC-MSMS ham verileri MaxQuant yazılımı ile işlenmiş ve Andromeda arama motoru ile insan UniProt veri tabanında aranmıştır. Grupların karşılaştırılması, ısı haritaları çizmek için bir R işlevi olan pHeatmap üzerinde hazırlanan ısı haritaları aracılığıyla görselleştirildi. Zenginleştirilmiş yollar, her bir proteinin Uniprot kodlarının çevrimiçi haritalama aracı yolu DIP'ye girilmesiyle belirlenmiştir. Protein-protein etkileşim ağları, Toronto Üniversitesi'nin Entegre Etkileşimler Veritabanı aracılığıyla analiz edilmiştir. Sonuç olarak, Kalsiyum fruktoborat (CFB) bazı örneklerde apoptozla ilişkili proteinlerin ifadesini belirgin şekilde azaltarak güçlü bir biyolojik yanıt ortaya çıkarmış olabilir ve DNA onarımıyla ilişkili proteinlerin ifadesinde borik asitten daha belirgin bir değişikliğe neden olduğu görülmektedir. Bu değişiklikler çoğunlukla baskılama yönündedir (negatif değerler), bu da potansiyel olarak DNA onarım yollarını baskılayabileceğini düşündürmektedir. CaF grubunun apoptoz ve DNA onarım yolakları ile ilişkili olduğu bulunmuştur.

Abstract

A2780 ovarian cancer cell line was used to investigate the effects of Boric Acid (BA) and Calcium Fructoborate (CaF) on the proteomic profile of cells in terms of apoptotic pathways and DNA damage/repair pathways. Cell viability and toxicity were determined by the MTT method. Cells were treated with BA and CaF according to the MTT results and collected for protein isolation. Protein isolation was performed from the cell line. Peptides from each sample were obtained for bottom-up proteomic analysis and run on a LC–MS/MS (DionexUltimate 3000 RSLCnano system/Easy Spray/Q-Exactive Plus, Thermo Scientific, USA) The LC-MSMS raw data were processed with the MaxQuant software and searched with Andromeda search engine against the human UniProt database. Comparison of groups were visualized through heatmaps that were prepared on the pHeatmap, an R function to draw heatmaps. Enriched pathways were determined through inputting the Uniprot codes of each protein on the online mapping tool path DIP. Protein-protein interaction networks were analysed through the Integrated Interactions Database of University of Toronto. As a result, Calcium fructoborate (CFB) may have elicited a strong biological response by markedly reducing the expression of apoptosis-related proteins in some samples and appears to cause a more pronounced change in the expression of DNA repair-related proteins than boric acid. These changes are mostly in the direction of repression (negative values), suggesting that it could potentially suppress DNA repair pathways. The CaF group was found to be associated with apoptosis and DNA repair pathways.

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Bu Makaleye Atıf Yap

Güler, Ü., Özcan, S. S., Yapar, R., Onat, B., Korkmaz, M., Salih, B. (2025). Yumurtalık kanserinde borik asit ve kalsiyum fruktoboratın etkisi. Meandros Medical And Dental Journal, Volume: 26 Issue: 4. https://doi.org/10.69601/meandrosmdj.1732132

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