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Dergiler / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2026 / Cilt: 8 Sayı: 2

Laktoperoksidaz Enziminin Farklı Kaynaklardan Saflaştırılması: Kapsaisin ve Pirogallol’ün İnhibisyon Profili

Lactoperoxidase Enzyme Purified from Different Milk Sources: Inhibition Profile of Capsaicin and Pyrogallol

Zeynep Köksal0000-0001-8203-4623
Sayfa
159–165
Yayın Tarihi
2018-06-30
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Peroksidazlar (POD), gıda ve ilaç endüstrisi başta olmak üzere metabolik fonksiyonlar, enzimatik

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Peroksidazlar (POD), gıda ve ilaç endüstrisi başta olmak üzere metabolik fonksiyonlar, enzimatik
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Temel Bulgular & Veri:Peroksidazlar (POD), gıda ve ilaç endüstrisi başta olmak üzere metabolik fonksiyonlar, enzimatik
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Sonuç & Katkı:Peroksidazlar (POD), gıda ve ilaç endüstrisi başta olmak üzere metabolik fonksiyonlar, enzimatik
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Enzim inhibisyonu · kapsaisin · laktoperoksidaz

Özet

Peroksidazlar (POD), gıda ve ilaç endüstrisi başta olmak üzere metabolik fonksiyonlar, enzimatik

Abstract

Peroxidases (POD) have an important use in metabolic functions, enzymatic reactions and clinical diagnoses, especially in the food and pharmaceutical industry. Mammalian POD enzymes, Lactoperoxidase (LPO) (hydrogen peroxide oxidoreductase E.C. 1.11.1.7) is localized in milk, saliva and tears, while myeloperoxidase is localized in leukocytes and platelets. LPO enzyme catalyses the conversion of hypothiocyanate with antibacterial properties of thiocyanate in the presence of hydrogen peroxide. The purpose of this study was to determine the in vitro inhibition effects of capsaicin and pyrogallol on LPO enzyme purified from different milk sources (Bovine, buffalo, sheep and goat). To determine the inhibition effects of capsaicin and pyrogallol on LPO, LPO enzyme was purified from different mammalian milk and then, Lineweaver-Burk graphs were drawn for each inhibitor by measuring enzyme activities; Ki values and inhibition types were determined from these plotted graphs. The Ki values of capsaicin and pyrogallol were found in ranging of 0.0035-36.178 µM. Pyrogallol was shown the most effective inhibitor feature with a non-competitive inhibition type with 0.0035 ± 0.0012 μM Ki value on LPO enzyme purified from sheep milk.

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Bu Makaleye Atıf Yap

Köksal, Z. (2018). Laktoperoksidaz Enziminin Farklı Kaynaklardan Saflaştırılması: Kapsaisin ve Pirogallol’ün İnhibisyon Profili. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 8 Sayı: 2, 159–165. https://doi.org/10.21597/jist.428362

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