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Glutatyon S-Transferaz Enzim Aktivitesi Üzerine Amoksilin ve Vankomisin Hidroklorid Hidratın Etkisi: Bir in vitro çalışma

The Effects of Amoxicillin and Vancomycin Hydrochloride Hydrate on Glutathione S-Transferase Enzyme Activity: An in vitro study

Fikret Türkan0000-0002-0538-3157
Mehmet Nuri Atalar0000-0003-2993-2605
Sayfa
141–148
Yayın Tarihi
2018-06-30
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Glutatyon S-transferaz enzimleri kemoterapik ilaçlar, çevresel karsinojenler, endojen moleküller olmak

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Glutatyon S-transferaz enzimleri kemoterapik ilaçlar, çevresel karsinojenler, endojen moleküller olmak
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Temel Bulgular & Veri:Glutatyon S-transferaz enzimleri kemoterapik ilaçlar, çevresel karsinojenler, endojen moleküller olmak
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Sonuç & Katkı:Glutatyon S-transferaz enzimleri kemoterapik ilaçlar, çevresel karsinojenler, endojen moleküller olmak
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Amoksisilin · vankomisin hidroklorür · glutatyon s- transferaz

Özet

Glutatyon S-transferaz enzimleri kemoterapik ilaçlar, çevresel karsinojenler, endojen moleküller olmak

Abstract

Glutathione S-transferase (GST) enzymes detoxify a broad spectrum of xenobiotics, including chemotherapeutic drugs, environmental carcinogens, and endogenous molecules. Glutathione transferases catalyze the conversion of some metabolites into less toxic substances. Phase II reactions, which often result in detoxification, are conjugation reactions of glutathione through GST enzymes that protect the organism from the attacks of highly reactive electrophilic substances. Since the GST enzyme can metabolize toxic exogenous compounds, it has been extensively investigated in mammals. In this study, the GST enzyme was purified in human erythrocytes with a specific activity of 5.56 EU mg-1 protein and a yield of 2316.0 fold with 65% efficienc. SDS-polyacrylamide gel electrophoresis was performed to check the purity of the purified enzyme and the molecular mass was determined to be about 26 kDa. The IC50 and Ki values for amoxycillin and vancomycin hydrochloride drugs on the purified GST enzyme were calculated and the type of inhibition was determined. As a result of the inhibition studies, we observed that these antibiotics inhibited the GST enzyme at low doses.

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Bu Makaleye Atıf Yap

Türkan, F., Atalar, M. N. (2018). Glutatyon S-Transferaz Enzim Aktivitesi Üzerine Amoksilin ve Vankomisin Hidroklorid Hidratın Etkisi: Bir in vitro çalışma. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 8 Sayı: 2, 141–148. https://doi.org/10.21597/jist.428354

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