KaydedilenlerDerginizi ekleyin

Dergiler / Hacettepe Journal of Biology and Chemistry / 2026 / Volume: 35 Issue: 2

Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption

Bahar Ergün(Hacettepe University, Department of Chemistry, Ankara, Turkey)
Ali Derazshamshir(Hacettepe University, Department of Chemistry, Ankara, Turkey)
Mehmet Odabaşı(Aksaray University, Department of Chemistry, Aksaray, Turkey)
Sayfa
—
Yayın Tarihi
2007-04-01
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

It was observed thatlysozyme could be repeatedly adsorbed and desorbed with the poly HEMA-MAH cyogel without significant loss ofadsorption capacity

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Poly 2-hydroxyethyl methacrylate-N-methacryloyl- L -histidine methyl ester [poly HEMA-MAH ] cryogel was preparedby bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column cryo-polymerization .
📊
Temel Bulgular & Veri:After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected poresof 10-50 μm size.
💡
Sonuç & Katkı:It was observed thatlysozyme could be repeatedly adsorbed and desorbed with the poly HEMA-MAH cyogel without significant loss ofadsorption capacity
🔬Tür:Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:cryogel · lysozyme · immobilized metal affinity chromatography

Abstract

Poly 2-hydroxyethyl methacrylate-N-methacryloyl- L -histidine methyl ester [poly HEMA-MAH ] cryogel was preparedby bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column cryo-polymerization . After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected poresof 10-50 μm size. Then the monolithic cryogel was loaded with Fe III ions to form the metal chelate with poly HEMAMAH cryogel. Poly HEMA-MAH cryogel was characterized by swelling studies, FTIR, scanning electron microscopy,and elemental analysis. The equilibrium swelling degree of the poly HEMA-MAH monolithic cryogel was 2.82 g H2O/gcryogel. Poly HEMA-MAH cryogel containing 36.4 μmol MAH/g was used in the adsorption/desorption of lysozymefrom aqueous solutions. The nonspecific adsorption of lysozyme was very low 1.1 mg/g . The maximum amount oflysozyme adsorption from aqueous solution in phosphate buffer was 40.7 mg/g at pH 7.0. It was observed thatlysozyme could be repeatedly adsorbed and desorbed with the poly HEMA-MAH cyogel without significant loss ofadsorption capacity

🔖

Bu Makaleye Atıf Yap

Ergün, B., Derazshamshir, A., Odabaşı, M. (2007). Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption. Hacettepe Journal of Biology and Chemistry, Volume: 35 Issue: 2. https://ajindex.com/articles/1271174

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (28)

  1. M. Wilchek, T. Miron, React. Func. Polym. 41 (1999) 263.
  2. A. Denizli, E. Pişkin, J. Biochem. Biophys. Method 49 (2001) 391.
  3. L. Uzun, H. Yavuz, R. Say, A. Ersöz, A. Denizli, Ind. Eng. Chem. Res. 43 (2004) 6507.
  4. S. Emir, R. Say, H. Yavuz, A. Denizli, Biotechnol. Prog. 20 (2004) 223.
  5. H. Yavuz, A. Denizli, Macromol. Biosci. 4 (2004) 84.
  6. S. Oncel, L. Uzun, B. Garipcan, A. Denizli, Ind. Eng. Chem. Res. 44 (2005)7049.
  7. M.N. Gupta, S. Jain, I. Roy, Biotechnol. Prog. 18 (2002) 78.
  8. A. Denizli, B. Salih, E. Pişkin. J. Chromatogr. A 731 (1996) 57.
  9. G. Tishchenko, J. Dybal, K. Meszaroova, Z. Sedlakova, M. Bleha. J. Chromatogr. A 954 (2002) 115.
  10. M.Y. Arıca, H. Testereci, A. Denizli. J. Chromatogr. A 799 (1998) 83.
  11. F. Denizli, A. Denizli, M.Y. Arıca. Polym. Int. 48 (1999) 360.
  12. T.T. Yip, T. Hutchens. W. Mol. Biotechnol. 1 (1994) 151.
  13. R. Ghosh, Z.F. Cui, J. Membr. Sci. 167 (2000) 47.
  14. R. Ghosh, S.S. Silva, C.F. Cui, Biochem. Eng. J. 6 (2000) 19.
  15. S. Das, S. Banerjee, J. Dasgupta, Chemotherapy 38 (1992) 350.
  16. F. Cartei, G. Cartei, V. Ceschia, S. Pacor, G. Sava, Curr. Therap. Res. Clin. Exp. 50 (1991) 530.
  17. F. Murakami, T. Sasaki, T. Sugahara, Cytotechnol- ogy 24 (1997) 177.
  18. Garipcan, B.; Denizli, A. Macromol Biosci 2002, 2, 135.
  19. J.W. Wong, R.L. Albriqht, N-H.L. Wanq, Sep. Purif. Method 20 (1991) 49.
  20. G.D. Fasman, Handbook of Biochemistry and Mo- lecular Biology, CRC Press, Boca Raton FL, 1977.
  21. K.D. Jurgens, R. Baumann, Eur. Biophys. J. 12 (1985) 217.
  22. S. Senel, A. Kassab, Y. Arica, R. Say, A. Denizli, Colloid Surf. B 24 (2002) 265–275.
  23. E. Valdman, L. Erijman, F.L.P. Pessoa, S.G.F. Leite, Process Biochem. 36 (2001) 869.
  24. R.M. Birch, C. O’Byrne, I.R. Booth, P. Cash, Proteomics 3 (2003) 764.
  25. C.Y. Wu, S.Y. Suen, S.C. Chen, J.H. Tzeng, J. Chromatogr. A 996 (2003) 53.
  26. H. Yavuz, A. Denizli, Macromol. Biosci. 5 (2005) 39.
  27. A. Denizli, S. Senel, Y. Arıca, Colloids Surf. B 11 (1998) 113.
  28. E. Valdman, L. Erijman, F.L.P. Pessoa, S.G.F. Leite, Process Biochem. 36 (2001) 869.