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Journals / Hacettepe Journal of Biology and Chemistry / 2026 / Volume: 35 Issue: 2

Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption

Bahar Ergün(Hacettepe University, Department of Chemistry, Ankara, Turkey)
Ali Derazshamshir(Hacettepe University, Department of Chemistry, Ankara, Turkey)
Mehmet Odabaşı(Aksaray University, Department of Chemistry, Aksaray, Turkey)
Pages
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Publication Date
2007-04-01
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

It was observed thatlysozyme could be repeatedly adsorbed and desorbed with the poly HEMA-MAH cyogel without significant loss ofadsorption capacity

📌 3 Key Takeaways

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Objective & Hypothesis:Poly 2-hydroxyethyl methacrylate-N-methacryloyl- L -histidine methyl ester [poly HEMA-MAH ] cryogel was preparedby bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column cryo-polymerization .
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Key Findings & Data:After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected poresof 10-50 μm size.
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Conclusion & Impact:It was observed thatlysozyme could be repeatedly adsorbed and desorbed with the poly HEMA-MAH cyogel without significant loss ofadsorption capacity
🔬Design:Article
📍Scope:Comprehensive Sample
⚙️Variables:cryogel · lysozyme · immobilized metal affinity chromatography

Abstract

Poly 2-hydroxyethyl methacrylate-N-methacryloyl- L -histidine methyl ester [poly HEMA-MAH ] cryogel was preparedby bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column cryo-polymerization . After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected poresof 10-50 μm size. Then the monolithic cryogel was loaded with Fe III ions to form the metal chelate with poly HEMAMAH cryogel. Poly HEMA-MAH cryogel was characterized by swelling studies, FTIR, scanning electron microscopy,and elemental analysis. The equilibrium swelling degree of the poly HEMA-MAH monolithic cryogel was 2.82 g H2O/gcryogel. Poly HEMA-MAH cryogel containing 36.4 μmol MAH/g was used in the adsorption/desorption of lysozymefrom aqueous solutions. The nonspecific adsorption of lysozyme was very low 1.1 mg/g . The maximum amount oflysozyme adsorption from aqueous solution in phosphate buffer was 40.7 mg/g at pH 7.0. It was observed thatlysozyme could be repeatedly adsorbed and desorbed with the poly HEMA-MAH cyogel without significant loss ofadsorption capacity

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Cite This Article

Ergün, B., Derazshamshir, A., Odabaşı, M. (2007). Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption. Hacettepe Journal of Biology and Chemistry, Volume: 35 Issue: 2. https://ajindex.com/articles/1271174

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