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Dergiler / Anadolu Tarım Bilimleri Dergisi / 2026 / Cilt: 41

In vitro Çoğaltılan Hypericum aucheri Bitkilerinin Flow Sitometri Temelli Değerlendirilmesi

A Flow Cytometry-Based Evaluation of In vitro Micropropagated Hypericum aucheri Plants

Gülru Yücel(ONDOKUZ MAYIS ÜNİVERSİTESİ)0000-0001-9785-929X
Ebru Cambaz(ÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ)0000-0001-7301-9288
Çağla Arslan(ONDOKUZ MAYIS ÜNİVERSİTESİ)0009-0004-4072-1897
Batuhan Tuna(NAMIK KEMAL ÜNİVERSİTESİ)0000-0003-4466-4175
Aleyna Kandemir(ÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ)0009-0006-0648-8950
Ersin Karabacak(ÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ)0000-0002-5784-6803
Nurşen Çördük(ÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ)0000-0001-8499-4847
Sayfa
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Yayın Tarihi
2026-09-15
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Flow sitometri sonuçlarına göre, analiz edilen örneklerin ortalama nükleer DNA içeriği 0.70 pg/2C to 0.75 pg/2C aralığında ve aynı ploidi düzeyine sahip olduğu tespit edilmiştir.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Hypericum aucheri, sekonder metabolit açısından potansiyeli olan önemli bir bitki türüdür.
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Temel Bulgular & Veri:Ayrıca elde edilen veriler neticesinde analiz edilmiş olan örneklerin genetik stabilitesi hakkında ön değerlendirme yapabilmektir.
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Sonuç & Katkı:Flow sitometri sonuçlarına göre, analiz edilen örneklerin ortalama nükleer DNA içeriği 0.70 pg/2C to 0.75 pg/2C aralığında ve aynı ploidi düzeyine sahip olduğu tespit edilmiştir.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Hypericum aucheri · Mikroçoğaltım · Bitki doku kültürü

Özet

Hypericum aucheri, sekonder metabolit açısından potansiyeli olan önemli bir bitki türüdür. Bu çalışmanın temel amacı, mikroçoğaltım ile üretilen H. aucheri bitkilerinde çekirdek DNA içeriğini flow sitometri yöntemi ile tespit etmek ve kromozom sayısını saptamaktır. Ayrıca elde edilen veriler neticesinde analiz edilmiş olan örneklerin genetik stabilitesi hakkında ön değerlendirme yapabilmektir. Flow sitometri sonuçlarına göre, analiz edilen örneklerin ortalama nükleer DNA içeriği 0.70 pg/2C to 0.75 pg/2C aralığında ve aynı ploidi düzeyine sahip olduğu tespit edilmiştir. Çekirdek DNA miktarındaki farklılık istatistiksel olarak anlamlı bulunmamıştır. Bu durum analiz edilen örnekler arasında benzerlik olduğunu göstermekte olup, potansiyel bir genetik stabilite olabileceğini düşündürmektedir. Fakat gelecekte yapılacak çalışmalarda, moleküler düzeyde genetik stabilitenin araştırılması ve rejenerasyon yoluyla elde edilen bitkilerin stabilitesinin daha kapsamlı bir şekilde değerlendirilmesi için moleküler markör tekniklerinin kullanılması ve örnek sayısının arttırılması önerilmektedir.

Abstract

Hypericum aucheri is an important plant species with significant potential as a source of secondary metabolites. The main aim of this study was to determine nuclear DNA content of the H. aucheri samples produced by micropropagation using flow cytometry and detection of the chromosome numbers. Furthermore, based on the obtained data, make a preliminary assessment regarding the genetic stability of the analyzed samples. According to the flow cytometric results, the mean nuclear DNA content of the analyzed samples varied from 0.70 pg/2C to 0.75 pg/2C, all sharing expected same ploidy level. Differences among H. aucheri samples were not statistically significant. In this respect, there is similarity among the samples analyzed which may indicate potential genetic stability. However, for future studies, it is recommended to employ molecular marker techniques and increase the sample size to confirm genetic stability at the molecular level and provide a more comprehensive assessment of the stability of regenerated plants.

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Bu Makaleye Atıf Yap

Yücel, G., Cambaz, E., Arslan, Ç., Tuna, B., Kandemir, A., Karabacak, E., Çördük, N. (2026). In vitro Çoğaltılan Hypericum aucheri Bitkilerinin Flow Sitometri Temelli Değerlendirilmesi. Anadolu Tarım Bilimleri Dergisi, Cilt: 41. https://doi.org/10.7161/omuanajas.1948942

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