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Dergiler / Experimed / 2026 / Volume: 14 Issue: 2

Effect of Lemon Verbena Polyphenol on Glycerol Channel Aquaporin 7 Expression in 3T3-L1 Adipocytes

Orkide Palabıyık(TRAKYA UNIVERSITY, HEALTH SERVICES VOCATIONAL SCHOOL)0000-0002-3488-3740
Emine Kılıç Toprak(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF PHYSIOLOGY)0000-0002-8795-0185
Deniz Şumnulu(TRAKYA UNIVERSITY, TECHNOLOGY RESEARCH AND DEVELOPMENT APPLICATION AND RESEARCH CENTER)0009-0009-0693-3569
Jülide Tozkır(TRAKYA UNIVERSITY, HEALTH SERVICES VOCATIONAL SCHOOL)0000-0002-8436-8309
Ayşegül Çört(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF MEDICAL BIOCHEMISTRY)0000-0001-8946-7173
Sayfa
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Yayın Tarihi
2024-08-26
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Approaches to regulate AQP7 gene expression in adipose tissue using plant-derived polyphonic compounds are considered a healthy and innovative approach to combat and manage metabolic diseases, including obesity.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: Polyphenols are of great interest in obesity prevention approaches.
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Temel Bulgular & Veri:The aquaglyceroporin 7 (AQP7) channel is involved in the transport of glycerol across cell membranes in adipose tissue.
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Sonuç & Katkı:Approaches to regulate AQP7 gene expression in adipose tissue using plant-derived polyphonic compounds are considered a healthy and innovative approach to combat and manage metabolic diseases, including obesity.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Polyphenol · adipocyte · aquaglyceroporin

Abstract

Objective: Polyphenols are of great interest in obesity prevention approaches. The aquaglyceroporin 7 (AQP7) channel is involved in the transport of glycerol across cell membranes in adipose tissue. This study aimed to explore how lemon verbena (LV) polyphenols affect the expression of the glycerol channels AQP7 and perilipin 1 (PLIN1) in 3T3-L1 hypertrophic adipocytes. Materials and Methods: Hypertrophic adipocyte cells (H) were treated with LV at two different doses of 200 µg/mL (H-LV200) and 400 µg/mL (H-LV400). In addition, 0.1 µM β3-AR agonist (CL316243) and 0.1 µM β3-AR antagonist (L7483337) were applied to the cells at both doses. AQP7 and PLIN1 gene expressions were determined by real time-polymerase chain reaction (RT-PCR), and glycerol levels were determined by enzyme-linked immunosorbent assay (ELISA). Results: Hypertrophic adipocytes showed increased AQP7 and PLIN1 gene expression and glycerol content compared with the control group. H-LV200 and CL316243 treatment together increased AQP7 gene expression, whereas H-LV400 and L7483337 treatment together decreased AQP7 gene expression. Conclusion: The data indicated that both doses of LV inhibited glycerol production by suppressing AQP7 and PLIN1 gene expression. Approaches to regulate AQP7 gene expression in adipose tissue using plant-derived polyphonic compounds are considered a healthy and innovative approach to combat and manage metabolic diseases, including obesity.

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Bu Makaleye Atıf Yap

Palabıyık, O., Toprak, E. K., Şumnulu, D., Tozkır, J., Çört, A. (2024). Effect of Lemon Verbena Polyphenol on Glycerol Channel Aquaporin 7 Expression in 3T3-L1 Adipocytes. Experimed, Volume: 14 Issue: 2. https://doi.org/10.26650/experimed.1479944

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