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Dergiler / Marmara Medical Journal / 2026 / Volume: 39 Issue: 2

Single-housing conditions result in higher running distance than per capita running distance in pair-housing conditions, without affecting cortisol and inflammatory indices in female rats

Ozge Dindar(TRAKYA UNIVERSITY)0000-0002-8808-6376
Muhammed Ali Aydın(Trakya University)0000-0002-3741-4499
Ebru Taştekin(Trakya University)0000-0002-7686-7765
Orkide Palabıyık(Trakya University)0000-0002-3488-3740
Selçuk Korkmaz(Trakya University)0000-0003-4632-6850
Selma Arzu Vardar(Trakya University)0000-0002-1073-1718
Sayfa
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Yayın Tarihi
2026-05-21
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Conclusion: Female rats subjected to voluntary physical activity under single-housing conditions may exhibit higher activity levels than pair-housing rats, but single-housing and pair-housing conditions were similarly effective in terms of stress and exercise-induced cardiac hypertrophy.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: Voluntary exercise is frequently used in experimental exercise studies, but it is not known whether isolating animals in this method affects stress.
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Temel Bulgular & Veri:Cortisol in serum, interleukin 1β (IL-1β), and tumor necrosis factor α (TNFα) in serum and left ventricle were determined to evaluate the stress.
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Sonuç & Katkı:Conclusion: Female rats subjected to voluntary physical activity under single-housing conditions may exhibit higher activity levels than pair-housing rats, but single-housing and pair-housing conditions were similarly effective in terms of stress and exercise-induced cardiac hypertrophy.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Voluntary physical activity · Cardiac hypertrophy · Exercise

Abstract

Objective: Voluntary exercise is frequently used in experimental exercise studies, but it is not known whether isolating animals in this method affects stress. The aim of this study was to compare voluntary physical activity and stress-related factors between single and pair-housed female rats. Materials and Methods: Rats were divided into control single (C-S) and control double (C-D), physically active-single (PAS), and physically active-double (PA-D) groups, and voluntary physical activity was recorded for eight weeks. Cortisol in serum, interleukin 1β (IL-1β), and tumor necrosis factor α (TNFα) in serum and left ventricle were determined to evaluate the stress. Cardiomyocyte morphology was assessed to understand activity-induced cardiac hypertrophy. Results: The mean weekly voluntary activities of the PA-S group were higher than those of the PA-D group from week 3 to week 8 (p<0.05 for all). Cortisol, IL-1β, and TNFα levels were similar in the PA-S and PA-D groups (p>0.05). Cardiomyocyte diameters were similar in the PA-S and PA-D groups (p>0.05), and the data of both groups were higher than the control group (P<0.001 for all). Conclusion: Female rats subjected to voluntary physical activity under single-housing conditions may exhibit higher activity levels than pair-housing rats, but single-housing and pair-housing conditions were similarly effective in terms of stress and exercise-induced cardiac hypertrophy.

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Bu Makaleye Atıf Yap

Dindar, O., Aydın, M. A., Taştekin, E., Palabıyık, O., Korkmaz, S., Vardar, S. A. (2026). Single-housing conditions result in higher running distance than per capita running distance in pair-housing conditions, without affecting cortisol and inflammatory indices in female rats. Marmara Medical Journal, Volume: 39 Issue: 2. https://doi.org/10.5472/marumj.1954066

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