SavedAdd your journal

Journals / CURRENT THORACIC SURGERY / 2017 / Cilt: 2 Sayı: 1

Upregulated mRNA expression of mTOR in surgically resected early stage non-small cell lung carcinoma: a potential molecular targeted therapy

Muhammet Sayan
Ali Çelik
Şevki Mustafa Demiröz
Hacer İlke Önen
Nalan Akyürek
Ece Konaç
İsmail Cüneyt Kurul
Abdullah İrfan Taştepe
Pages
5–10
Publication Date
2017-04-16
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results: Our findings revealed a statistically significant increase of mTOR expression on mRNA levels (P < 0.05).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background: Non-small cell lung cancer (NSCLC) comprises about 85% of all lung cancers.
📊
Key Findings & Data:PI3K/AKT/MTOR which is thought to be efficacious in the development of many cancer which controls the expression of many genes playing an important role in cell proliferation, metastasis, resistance to apoptosis and angiogenesis.
💡
Conclusion & Impact:Results: Our findings revealed a statistically significant increase of mTOR expression on mRNA levels (P < 0.05).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:non-small cell lung cancer · mRNA expression · AKT1

Abstract

Background: Non-small cell lung cancer (NSCLC) comprises about 85% of all lung cancers. Although many attempts for early detection and treatment, prognosis of NSCLC is still poor. &nbsp; In recent years the pathways and the genes that play role in lung cancer development were researched widely. PI3K/AKT/MTOR which is thought to be efficacious in the development of many cancer which controls the expression of many genes playing an important role in cell proliferation, metastasis, resistance to apoptosis and angiogenesis. Also the increase in CCND1 expression was shown in several cancer types. The aim of this study is to search the mRNA expression profile of AKT, MTOR and CCND1 genes which has been thought to play role in NSCLC development, and their expression in different pathological stages and histological types of the disease. Materials and Methods: Forty-four NSCLC patients who didn’t get neoadjuvant therapy were included in this study. The samples from tumor and matched normal lung tissue were obtained from resection specimens (lobectomy or pneumonectomy). Total cellular RNA was isolated from the samples. The mRNA expression levels of AKT1 , mTOR and CCND1 genes were measured by quantitative real-time polymerase chain reaction (qPCR). Results: Our findings revealed a statistically significant increase of mTOR expression on mRNA levels (P < 0.05). Although AKT and CCND1 expression slightly increased in malign tissues, these changes in the expression were not significant (P > 0.05). &nbsp; The mRNA expression of mTOR was also upregulated and it was statistically significant for early stage disease and adenocarcinoma subtype (P < 0.05). Conclusions: Inhibition of mTOR gene expression at mRNA level might be potential target for future treatment strategies of NSCLC.

🔖

Cite This Article

Sayan, M., Çelik, A., Demiröz, Ş. M., Önen, H. İ., Akyürek, N., Konaç, E., Kurul, İ. C., Taştepe, A. İ. (2017). Upregulated mRNA expression of mTOR in surgically resected early stage non-small cell lung carcinoma: a potential molecular targeted therapy. CURRENT THORACIC SURGERY, Cilt: 2 Sayı: 1, 5–10. https://ajindex.com/articles/984614

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (18)

  1. Deffebach ME, Humphrey L. Lung Cancer Screening. Surg Clin North Am 2015; 95: 967-78.
  2. Hu Q, Zhou Y, Ying K, Ruan W. IGFBP, a novel target of lung cancer? Clin Chim Acta 2017; 466: 172-7.
  3. Memmott RM, Dennis PA. The role of the Akt/mTOR pathway in tobacco carcinogen–induced lung tumorigenesis. Clin Cancer Res 2010; 16: 4-10.
  4. Luo X, Zang X, Yang L, Huang J, Liang F, Rodriguez-Canales J, et al. Comprehensive Computational Pathological Image Analysis Predicts Lung Cancer Prognosis. J Thorac Oncol 2017; 12: 501-9.
  5. Vooder T, Metspalu A. Investigating gene expression profile of non-small cell lung cancer. Cent Eur J Med 2011; 6: 608-15.
  6. Bartholomeusz C, Gonzalez-Angulo AM. Targeting the PI3K signaling pathway in cancer therapy. Expert Opin Ther Targets 2012; 16: 121-30.
  7. Fumarola C, Bonelli MA, Petronini PG, Alfieri RR. Targeting PI3K/AKT/mTOR pathway in non small cell lung cancer. Biochem Pharmacol 2014; 90:197-207.
  8. Caron E, Ghosh S, Matsuoka Y, Ashton-Beaucage D, Therrien M, Lemieux S, et al. A comprehensive map of the mTOR signaling network. Mol Syst Biol 2010; 6: 453.
  9. Liu Z, Wang L, Zhang LN, Wang Y, Yue WT, Li Q. Expression and clinical significance of mTOR in surgically resected non-small cell lung cancer tissues: a case control study. Asian Pac J Cancer Prev 2012; 13: 6139-44.
  10. Seeliger H, Guba M, Kleespies A, Jauch KW, Bruns CJ. Role of mTOR in solid tumor systems: a therapeutical target against primary tumor growth, metastases, and angiogenesis. Cancer Metastasis Rev 2007; 26: 611-21.
  11. Law M, Forrester E, Chytil A, Corsino P, Green G, Davis B, et al. Rapamycin disrupts cyclin/cyclin-dependent kinase/p21/proliferating cell nuclear antigen complexes and cyclin D1 reverses rapamycin action by stabilizing these complexes. Cancer Res 2006; 66: 1070-80.
  12. Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 2002; 30: e36.
  13. Langer CJ, Besse B, Gualberto A, Brambilla E, Soria JC. The evolving role of histology in the management of advanced non-small-cell lung cancer, J Clin Oncol 2010; 28: 5311-20.
  14. Liloglou T, Bediaga NG, Brown BR, Field JK, Davies MP. Epigenetic biomarkers in lung cancer. Cancer Lett 2014; 342: 200-12.
  15. Yue W, Wang X, Wang Y. The Relationship between the PI3K/Akt/mTOR Signal Transduction Pathway and Non-small Cell Lung Cancer. Zhongguo Fei Ai Za Zhi 2009; 12: 312-5.
  16. Wang L, Xu S, Yue W, Zhao X, Zhang L, Wang Y. Expression and clinical significance of mTOR and PTEN in non-small cell lung cancer. Zhongguo Fei Ai Za Zhi 2010; 13: 717-21.
  17. Li R, An SJ, Chen ZH, Zhang GC, Zhu JQ, Nie Q, et al. Expression of cyclin D1 splice variants is differentially associated with outcome in non-small cell lung cancer patients. Hum Pathol 2008; 39: 1792-801.
  18. Matsubara H, Sakakibara K, Kunimitsu T, Matsuoka H, Kato K, Oyachi N, et al. Non-small cell lung carcinoma therapy using mTOR-siRNA. Int J Clin Exp Pathol 2012; 5: 119-25.