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Journals / Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi / 2023 / Cilt: 12 Sayı: 2

Investigation of Antibiotic Resistance Genes in Clinical Acinetobacter baumannii and Pseudomonas.aeruginosa Strains Isolated From Trabzon Fatih State Hospital

Trabzon Fatih Devlet Hastanesinden İzole Edilen Klinik Acinetobacter baumannii ve Pseudomonas aeruginosa Suşlarında Antibiyotik Direnç Genlerinin Araştırılması

Esma AKYILDIZ
Ayşegül SARAL
Tuba KÖSE
Fatih Şaban BERİŞ
Azer ÖZAD DÜZGÜN
Pages
450–455
Publication Date
2023-06-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study demonstrates that OXA-23 is responsible for carbapenem resistance in CRPA (carbapenem resistant Pseudomonas aeruginosa) and CRAB (carbapenem resistant Acinetobacter baumannii) isolates.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of the study was to investigate the antibiotic susceptibility profile of Acinetobacter baumannii, and Pseudomonas aeruginosa isolates and to determine the presence of resistance genes in these isolates.The determination of resistance genes was assessed using the PCR method.
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Key Findings & Data:Imipenem and meropenem resistance in Acinetobacter baumannii isolates were 93.2% and 91%, respectively.
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Conclusion & Impact:This study demonstrates that OXA-23 is responsible for carbapenem resistance in CRPA (carbapenem resistant Pseudomonas aeruginosa) and CRAB (carbapenem resistant Acinetobacter baumannii) isolates.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Acinetobacter baumannii · Karbapenem · OXA-23

Abstract

The aim of the study was to investigate the antibiotic susceptibility profile of Acinetobacter baumannii, and Pseudomonas aeruginosa isolates and to determine the presence of resistance genes in these isolates.The determination of resistance genes was assessed using the PCR method. Imipenem and meropenem resistance in Acinetobacter baumannii isolates were 93.2% and 91%, respectively. The imipenem resistance rate was 53.9% and meropenem resistance rate was 38.5% in Pseudomonas aeruginosa isolates. Of the genes sought among Pseudomonas aeruginosa isolates, only OXA-23 was detected in one isolate. Among Acinetobacter baumannii isolates, OXA-24 was detected in one isolate and OXA-23 in 40 isolates. This study demonstrates that OXA-23 is responsible for carbapenem resistance in CRPA (carbapenem resistant Pseudomonas aeruginosa) and CRAB (carbapenem resistant Acinetobacter baumannii) isolates.

Özet

Bu çalışmanın amacı Acinetobacter baumannii ve Pseudomonas aeruginosa izolatlarının antibiyotik duyarlılık profilini araştırmak ve bu izolatlarda direnç genlerinin varlığını belirlemektir. Direnç genlerinin tespiti PCR yöntemi ile değerlendirildi. Acinetobacter baumannii izolatlarında imipenem ve meropenem direnci sırasıyla %93,2 ve %91 olduğu görüldü. P. aeruginosa izolatlarında imipenem direnç oranı %53,9 ve meropenem direnç oranı %38,5 olarak belirlendi. Pseudomonas aeruginosa izolatları arasında aranan genlerden bir izolatta sadece OXA-23 tespit edildi. Acinetobacter baumannii izolatlarından bir izolatta OXA-24, 40 izolatta OXA-23 saptandı. Bu çalışma, CRPA (Karbapenem dirençli Pseudomonas aeruginosa) ve CRAB (Karbapenem dirençli Acinetobacter baumannii) izolatlarında karbapenem direncinden OXA-23'ün sorumlu olduğunu göstermektedir.

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Cite This Article

AKYILDIZ, E., SARAL, A., KÖSE, T., BERİŞ, F. Ş., DÜZGÜN, A. Ö. (2023). Investigation of Antibiotic Resistance Genes in Clinical Acinetobacter baumannii and Pseudomonas.aeruginosa Strains Isolated From Trabzon Fatih State Hospital. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi, Cilt: 12 Sayı: 2, 450–455. https://ajindex.com/articles/949542

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References (7)

  1. Wang, X. and Qin, L.J. (2019). “A review on Acinetobacter baumannii”. Journal of Acute Disease, 8 (1), 16-20.
  2. Özsoy, M, Sönmezer, M. and Kınıklı, S. (2019). “A recent problem at hospitals: treatment of multi-drug resistant Acinetobacter baumanii infections”. Anadolu Güncel Tıp Dergisi, 1 (2),37-41.
  3. Lee, C.R, Lee, J.H, Park, M, Park, K.S, Bae, I.K, Kim, Y.B, Cha, C.J, Jeong, B.C. and Lee, S.h. (2017). “Biology of Acinetobacter baumannii: pathogenesis, antibiotic resistance mechanisms, and prospective treatment options”. Frontiers in Cellular and Infection Microbiolology,13 (7), 55.https://doi.org 10.3389/fcimb.2017.00055 4. Demirci, M, Yigin, A, Demir, C. (2019). “Karbapeneme Direncli Acinetobacter baumannii Suşlarinda OXA Tipi Karbapenemaz Genlerinin Dagiliminin Gercek Zamanli Polimeraz Zincir Reaksiyonu Yontemiyle Incelenmesi”. KLIMIK Journal, 32, 123-126.
  4. Davoodi, S, Boroumand, M.A, Sepehriseresht, S. and Pourgholi, L. (2015). “Detection of VIM-and IMP-type metallo-beta-lactamase genes in Acinetobacter baumannii isolates from patients in two hospitals in Tehran”. Iranian Jounal of Biotechnology, 13(1), 63-67.
  5. Girija, S.A, Jayaseelan, V.P and Arumugam, P. (2018). “Prevalence of VIM-and GIM-producing Acinetobacter baumannii from patients with severe urinary tract infection”. Acta Microbiologica et Immunologica Hungarica, 65(4), 539-550.
  6. Kal Çakmaklıoğulları, E. and Kuru, C. (2019). “Antimicrobial Susceptibility of Pseudomonas aeruginosa Strains: Evaluation in Different Sample Types”.ANKEM Journal, 33 (2), 37-42. https://doi.org/ 10.5222/ankem.2019.197 8. Rocha, A.J, Barsottini, M.R.D.O, Rocha. R.R, Laurindo, V.M, Laurindo de Moraes, F.L and Rocha, S.L. (2019). “Pseudomonas aeruginosa: Virulence Factors and Antibiotic Resistance Genes”. Brazilian Archives of Biology and Technology, 62, e19180503. https://doi.org/ 10.1590/1678-4324-2019180503 9. Strateva, T. and Yordanov, D. (2009). “Pseudomonas aeruginosa–a phenomenon of bacterial resistance”. Jounal of Medical Microbiology, 58(9), 1133-1148.
  7. Petrova, A.P, Stanimirova, I.D, Ivanov. I.N, Petrov, M.M, Miteva-Katrandzhieva, T.M, Grivnev, V.I, Kardjeva, V.S, Kantardzhiev, T.V. and Murdjeva, M.A. (2017). “Carbapenemase production of clinical isolates Acinetobacter baum