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Journals / Gümüşhane Üniversitesi Fen Bilimleri Dergisi / 2023 / Cilt: 13 - Sayı: 1

First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye

Türkiye'den izole edilen qnr ve blaVIM-4-like üreten klinik Alcaligenes faecalis’in ilk raporu

Esma AKYILDIZ
Ayşegül SARAL
Tuba KÖSE
Mikail ARSLAN
Fatih Şaban BERİŞ
Azer ÖZAD DÜZGÜN
Pages
57–65
Publication Date
2023-01-15
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The blaVIM-4-like gene was present in this strain, according to the results of the PCR and DNA sequencing studies.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The study set out to look at the clinical strain of A. faecalis's antibiotic susceptibility pattern.
📊
Key Findings & Data:The blaVIM-4-like gene was present in this strain, according to the results of the PCR and DNA sequencing studies.
💡
Conclusion & Impact:In this study, This research, demonstrated the presence of the blaVIM-4-like gene for the first time in A. faecalis from Turkey.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:A. faecalis · qnrB · VIM-4

Abstract

The study set out to look at the clinical strain of A. faecalis's antibiotic susceptibility pattern. Both biochemical and molecular approaches were used to identify A.faecalis. The VITEK compact system was used to analyze the strain's antibiotic susceptibility profile. The use of PCR was evaluated to determine the presence of resistance genes. It was also determined whether resistance determinants could be transferred. It was determined that A. feacalis showed resistance to imipenem, cefepime, ceftazidime, gentamicin, amikacin, netilmicin, tobramicin, ciprofloxacin, and levofloxacin. The blaVIM-4-like gene was present in this strain, according to the results of the PCR and DNA sequencing studies. In contrast to VIM-4, this blaVIM-4-like gene showed one amino acid change (Ala265Val).Filter-mating experiments showed that the blaVIM-4-like gene cannot be transferred from A. faecalis to E. coli. In this study, This research, demonstrated the presence of the blaVIM-4-like gene for the first time in A. faecalis from Turkey.

Özet

Çalışmanın amacı, A. faecalis klinik suşunun antibiyotik duyarlılık paterninin araştırmaktır. A. faecalis hem biyokimyasal hem de moleküler yöntemlerle tanımlanmıştır. VITEK kompakt sistemi, suşun antibiyotik duyarlılık profilini analiz etmek için kullanıldı. Direnç genlerinin belirlenmesi, PCR yöntemi kullanılarak değerlendirildi. Ayrıca, direnç belirleyicilerinin aktarılabilirliği araştırıldı. A. feacalis'in imipenem, sefepim, seftazidim, gentamisin, amikasin, netilmisin, tobramisin, siprofloksasin ve levofloksasine direnç gösterdiği belirlendi. PCR ve DNA dizi analizleri, bu suşun blaVIM-4 benzeri gene sahip olduğunu ortaya çıkardı. Bu blaVIM-4-like gen, VIM-4'e kıyasla bir amino asit ikamesi (Ala265Val) sergiledi. Filtre eşleştirme deneyleri, blaVIM-4-like genin A. faecalis'ten E. coli'ye aktarılamayacağını gösterdi. Bu çalışmada, Türkiye'den A. faecalis'te ilk kez blaVIM-4-like genin varlığı ortaya konmuştur.

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Cite This Article

AKYILDIZ, E., SARAL, A., KÖSE, T., ARSLAN, M., BERİŞ, F. Ş., DÜZGÜN, A. Ö. (2023). First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, Cilt: 13 - Sayı: 1, 57–65. https://ajindex.com/articles/876862

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References (7)

  1. Adelowo, O.O., & Fagade, O.E. (2012). Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria. Turkish Journal of Biology, 36:37-45. https://doi.org/10.3906/biy-1010-145
  2. Adesoji, A.T., & Ogunjobi, A.A. (2016). Detection of extended spectrum beta-lactamases resistance genes among bacteria isolated from selected drinking water distribution channels in southwestern Nigeria. BioMed research international, 7149295. https://doi.org/10.1155/2016/7149295. Agersø, Y., & Sandvang, D. (2005). Class I integrons and tetracycline resistance genes in alcaligenes, arthrobacter, and pseudomonas spp. isolated from pigsties and manured soil. Applied And Environmental Microbiology, 71(12),7941-7. https://doi.org/10.1128/AEM.71.12.7941-7947.2005.
  3. Al Laham, N., Chavda, K.D., Cienfuegos-Gallet, A.V., Kreiswirth, B.N., & Chen, L. (2017). Genomic characterization of vim metallo-β-lactamase-producing Alcaligenes faecalis from Gaza, Palestine. Antimicrobial agents and chemotherapy, 61(11),e01499-17. https://doi.org/10.1128/AAC.01499-17.
  4. Ashwath, M.L., & Katner, H.P. (2005). Pancreatic abscess secondary to Alcaligenes faecalis. The American Journal of the Medical Sciences, 329(1),54-5. https://doi.org/10.1097/00000441-200501000-00011.
  5. Barlow, R.S, Fegan, N., & Gobius, K.S. (2008). A comparison of antibiotic resistance integrons in cattle from separate beef meat production systems at slaughter. Journal of Applied Microbiology, 104(3),651–58. https://doi.org/ 10.1111/j.1365-2672.2007.03572.x. Bush, K. (2010). Bench-to-bedside review: The role of beta-lactamases in antibiotic-resistant Gram-negative infections. Critical Care, 14(3),224. https://doi.org/10.1186/cc8892.
  6. Bush, K. (2018). Past and present perspectives on β-Lactamases. Antimicrobial Agents and Chemotherapy, 62:e01076-18. https://doi.org/10.1128/AAC.01076-18.
  7. Cattoir, V., Poirel, L., Rotimi, V., Soussy, C.J., & Nordmann, P. (2007). Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates, Journal of Antimicrobial Chemotherapy, 60(2),394-7. https://doi.org/ 10.1093/jac/dkm204. Celenza, G., Pellegrini, C., Caccamo, M., Segatore, B., Amicosante, G., & Perilli, M. (2006). Spread of blaCTX-M-type and blaPER-2 β-lactamase genes in clinical isolates from Bolivian hospitals, Journal of Antimicrobial Chemotherapy, 57 (5), 975-8. https://doi.org/10.1093/jac/d