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Journals / Veterinary Journal of Mehmet Akif Ersoy University / 2022 / Cilt: 7 Sayı: 3

An optimized protocol for the electroporation of NCI H929 multiple myeloma cells

Ayşe KIZILYER
Pages
188–194
Publication Date
2022-12-31
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results are expected to serve as a starting point and a guide for researchers.

📌 3 Key Takeaways

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Objective & Hypothesis:Multiple myeloma cell lines are difficult to transfect with non-viral nucleic acid delivery methods.
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Key Findings & Data:In this study, various parameters for NCI H929 human multiple myeloma cells are tested to generate an optimized electroporation protocol.
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Conclusion & Impact:These results are expected to serve as a starting point and a guide for researchers.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:electroporation · multiple myeloma · NCI H929

Abstract

Multiple myeloma cell lines are difficult to transfect with non-viral nucleic acid delivery methods. While electroporation is the most efficient tool for the transfection of most hard-to-transfect cells, human multiple myeloma cells differ in their permissiveness and each cell type require different electroporation conditions for an efficient transgene delivery. In this study, various parameters for NCI H929 human multiple myeloma cells are tested to generate an optimized electroporation protocol. Findings from this paper showed that besides the voltage and capacitance settings, cell count, the cell cycle status of cells, the amount of nucleic acid and removal of death cells all impacted the electroporation efficiency and viable cell count. These results are expected to serve as a starting point and a guide for researchers.

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Cite This Article

KIZILYER, A. (2022). An optimized protocol for the electroporation of NCI H929 multiple myeloma cells. Veterinary Journal of Mehmet Akif Ersoy University, Cilt: 7 Sayı: 3, 188–194. https://ajindex.com/articles/931167

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References (11)

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