KaydedilenlerDerginizi ekleyin

Dergiler / Veterinary Journal of Mehmet Akif Ersoy University / 2022 / Cilt: 7 Sayı: 3

An optimized protocol for the electroporation of NCI H929 multiple myeloma cells

Ayşe KIZILYER
Sayfa
188–194
Yayın Tarihi
2022-12-31
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

These results are expected to serve as a starting point and a guide for researchers.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Multiple myeloma cell lines are difficult to transfect with non-viral nucleic acid delivery methods.
📊
Temel Bulgular & Veri:In this study, various parameters for NCI H929 human multiple myeloma cells are tested to generate an optimized electroporation protocol.
💡
Sonuç & Katkı:These results are expected to serve as a starting point and a guide for researchers.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:electroporation · multiple myeloma · NCI H929

Abstract

Multiple myeloma cell lines are difficult to transfect with non-viral nucleic acid delivery methods. While electroporation is the most efficient tool for the transfection of most hard-to-transfect cells, human multiple myeloma cells differ in their permissiveness and each cell type require different electroporation conditions for an efficient transgene delivery. In this study, various parameters for NCI H929 human multiple myeloma cells are tested to generate an optimized electroporation protocol. Findings from this paper showed that besides the voltage and capacitance settings, cell count, the cell cycle status of cells, the amount of nucleic acid and removal of death cells all impacted the electroporation efficiency and viable cell count. These results are expected to serve as a starting point and a guide for researchers.

🔖

Bu Makaleye Atıf Yap

KIZILYER, A. (2022). An optimized protocol for the electroporation of NCI H929 multiple myeloma cells. Veterinary Journal of Mehmet Akif Ersoy University, Cilt: 7 Sayı: 3, 188–194. https://ajindex.com/articles/931167

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (11)

  1. Batista Napotnik, T., Polajžer, T., & Miklavčič, D. (2021). Cell death due to electroporation - A review. Bioelectrochemistry (Amsterdam, Netherlands), 141, 107871. https://doi.org/10.1016/j.bioelechem.2021.107871 Biorad. Gene Pulser Xcell Electroporation System Instruction Manual (Issue 62). https://www.bio-rad.com/sites/default/files/2022-01/10000148530.pdf
  2. Brito, J. L., Brown, N., & Morgan, G. J. (2010). Transfection of siRNAs in multiple myeloma cell lines. Methods Mol Biol, 623, 299–309. https://doi.org/10.1007/978-1-60761-588-0_19
  3. Brunner, S., Sauer, T., Carotta, S., Cotten, M., Saltik, M., & Wagner, E. (2000). Cell cycle dependence of gene transfer by lipoplex, polyplex and recombinant adenovirus. Gene Therapy, 7(5), 401–407. https://doi.org/10.1038/sj.gt.3301102
  4. Canoy, R. J., Andre, F., Shmakova, A., Wiels, J., Lipinski, M., Vassetzky, Y., & Germini, D. (2020). Easy and robust electrotransfection protocol for efficient ectopic gene expression and genome editing in human B cells. Gene Ther. https://doi.org/10.1038/s41434-020-00194-x
  5. Chong, Z. X., Yeap, S. K., & Ho, W. Y. (2021). Transfection types, methods and strategies: a technical review. PeerJ, 9, e11165–e11165. https://doi.org/10.7717/peerj.11165
  6. Fus-Kujawa, A., Prus, P., Bajdak-Rusinek, K., Teper, P., Gawron, K., Kowalczuk, A., & Sieron, A. L. (2021). An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro. Frontiers in Bioengineering and Biotechnology, 9. https://www.frontiersin.org/article/10.3389/fbioe.2021.701031
  7. Hattori, H., Tagawa, S., & Kitani, T. (1995). [Establishment and usage of human multiple myeloma cell line]. Nihon Rinsho, 53(3), 603–609. https://www.ncbi.nlm.nih.gov/pubmed/7699892
  8. Hsu, C. Y., & Uludag, H. (2012). A simple and rapid nonviral approach to efficiently transfect primary tissue-derived cells using polyethylenimine. Nat Protoc, 7(5), 935–945. https://doi.org/10.1038/nprot.2012.038
  9. Mizrahy, S., Hazan-Halevy, I., Dammes, N., Landesman-Milo, D., & Peer, D. (2017). Current Progress in Non-viral RNAi-Based Delivery Strategies to Lymphocytes. Molecular Therapy: The Journal of the American Society of Gene Therapy, 25(7), 1491–1500. https://doi.org/10.1016/j.ymthe.2017.03.001
  10. Oyeleye, O., Ogundeji, S., Ola, S., & Omitogun, O. (2016). Basics of animal cell culture: Foundation for modern science. Biotechnology and Molecular Biology Reviews, 11, 6–16. https://doi.org/10.5897/BMBR2016.0261
  11. Rols, M.-P. (2017). Parameters Affecting Cell Viability Following E