SavedAdd your journal

Journals / Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi / 2010 / Cilt: 7 Sayı: 1

ESTIMATION OF GENETIC DIFFERENCES AMONG GUPPIES, Poecilia reticulata by RAPD MARKERS

Lepistes Varyeteleri, Poecilia reticulata Arasındaki Genetik Farklılığın RAPD Markerlar ile İncelenmesi

Semra KÜÇÜK
Pages
65–70
Publication Date
2010-03-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Genetic similarities of all varieties were determined quietly high (minimum 0.670, maximum 0.948).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Sixty-four individuals that comprise 8 guppy varieties were used for genetic analysis.
📊
Key Findings & Data:Genetic difference between king and flamingo was the highest (0.604).
💡
Conclusion & Impact:Genetic similarities of all varieties were determined quietly high (minimum 0.670, maximum 0.948).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Lepistes · Poecilia reticulata · RAPD

Abstract

Sixty-four individuals that comprise 8 guppy varieties were used for genetic analysis. Genomic DNA of each individual was extracted from muscle tissues. DNA structure of each sample was examined by using random amplification of polymorphic DNA technique. Nine primers were worked in RAPD-polymerase chain reaction. Seven of them worked well having bands. Genetic similarities and differences for intraspecies and interspecies were calculated. Genetic difference between king and flamingo was the highest (0.604). Genetic similarities of all varieties were determined quietly high (minimum 0.670, maximum 0.948).

Özet

Sekiz lepistes varyetesi içeren 64 birey genetik analiz için kullanıldı. Genomik DNA kas dokusundan ekstrakte edildi. Her örneğine ait genomik DNA RAPD tekniği kullanılarak incelendi. Dokuz primer RAPD-PCR'da çalıştırıldı. Bu primerlerden sekiz tanesinde band tespit edildi. Varyeteler içi ve varyeteler arası genetik benzerlik ve farklılık hesaplandı. King ve flamingo arasındaki genetik farklılık en yüksek bulundu (0.604). Tüm varyetelerin genetik benzerlikleri oldukça yüksek olarak bulundu (minimum 0.670, maksimum 0.948).

🔖

Cite This Article

KÜÇÜK, S. (2010). ESTIMATION OF GENETIC DIFFERENCES AMONG GUPPIES, Poecilia reticulata by RAPD MARKERS. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, Cilt: 7 Sayı: 1, 65–70. https://ajindex.com/articles/920686

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Akhan, S., 2003, Üç farklı kuluçkahanedeki damızlık Foo C.L., Dinesh K.R., Lim T.M., Chan W.K., Phang, V.P.E., gökkuşağı alabalığı (Oncorhynchus mykiss Walbaum, ) stokları arasında genetik çeşitliliğin RAPD-PCR yöntemiyle belirlenmesi üzerine bir araştırma, Ege Üniversitesi Fen Bilimleri Enstitüsü Doktora Tezi, İzmir. 1995, Inheritance of RAPD markers in the guppy fish, Poecilia reticulata, Zoological Sciences, 12: 535-541.
  2. Goldberg D., Jackson K., Yehuda Y., Degani, G., 2000, Application of RAPD in the study of variations between African and American Cichlidae, Indian Journal Fisheries, 46: 307-312.
  3. Yayıncılık, İzmir.
  4. Hirschfeld D., Dhar A.K., Rask K., Alcivar-Warren, A., Bardakcı F., Skibinski, D.O.F., 1994,
  5. Application of the, Genetic diversity in the eastern oyster (Crassostrea virginica) from Massachusetts using RAPD technique, Journal of Shellfish Research, 18: 121-125.
  6. Barman H.K., Barat A., Yadav B.M., Banerjee S., Meher P.K., Reddy P.V.G.K., Jana, R.K., 2003, Genetic variation between four species of Indian major carps as Khoo G., Lim K.F., Gan D.K.Y., Chen F., Chan W., Lim T.M., Phang, V.P.E., 2002, Genetic diversity within and among feral populations and domesticated strains of guppy (Poecilia reticulata) in Singapore, Marine Biotechnology, 4: 367-378.
  7. Khoo G., Lim M.H., Suresh H., Gan D.K.Y., Lim K.F., Chen F., Chan W., Lim T.M., Phang, V.P.E., 2003, Genetic linkage maps of the guppy (Poecilia reticulata): assignment of RAPD markers to multipoint linkage groups, Marine Biotechnology, 5: 279-293.
  8. Klinbunga S., Ampayup P., Tassanakajon A., Jarayabhand P., Yoosukh, W., 2000, Development of species- specific markers of the tropical oyster (Crassostrea belcheri) in Thailand, Marine Biotechnology, 2: 476- 484. Koh, T.L., Khoo G., Fan L.Q., Phang, V.P.E., 1999, Genetic diversity among wild forms and cultivated varieties of Discus (Symphysodon spp.) as revealed by Random Amplified Polymorphic DNA (RAPD) fingerprinting, Aquaculture, 173: 485-497.
  9. Krane, D.E., Sternberg, D.C., Burton, G.A., 1999, Randomly amplified polymorphic DNA profile-based measures of genetic diversity in crayfish correlated with environmental impacts, Environmental Toxicology and Chemistry, 18: 504-508.
  10. Lehmann D., Hettwer H., Taraschewski, H., 2000, RAPD- PCR investigations of systematic relationships among four species of eels (Teleostei:Anguillidae), particularly Anguilla anguilla and A. Rostrat, Marine Biology, 137: 195-204.
  11. Leuzzi M.S.P., Almeida F.S., Orsi M.L., Sodre, L.M.K