SavedAdd your journal

Journals / Veterinary Sciences and Practices / 2019 / Cilt: 14 Sayı: 3

Detection of Plasma Profile of Pregnancy Associated Glycoproteins During Early Pregnancy in Pregnant Konya Merino Sheep

Gebe Konya Merinosu Koyunlarında Erken Gebelikte Gebelik İlişkili Glikoproteinlerin Plazma Profilinin Belirlenmesi

Yasemin Kaplan(GAP INTERNATIONAL AGRICULTURAL RESEARCH AND TRAINING CENTER)
Nihat Özyurtlu(DICLE UNIVERSITY, FACULTY OF VETERINARY, DEPARTMENT OF CLINICAL SCIENCES)0000-0001-8115-2222
Mehmet Köse(DICLE UNIVERSITY, FACULTY OF VETERINARY, DEPARTMENT OF CLINICAL SCIENCES)0000-0003-0070-8458
Mehmet Osman Atlı(DICLE UNIVERSITY, FACULTY OF VETERINARY, DEPARTMENT OF CLINICAL SCIENCES)0000-0001-9853-5334
İbrahim Küçükaslan(DICLE UNIVERSITY, FACULTY OF VETERINARY, DEPARTMENT OF CLINICAL SCIENCES)0000-0002-3458-4409
Mesut Kırbaş
Pages
307–314
Publication Date
2019-12-25
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to these results, it can be concluded that the commercial bovine ELISA-PAGs kit is good for detecting early pregnancy and monitoring fetoplacental unit well-being in Konya Merino ewes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the usability of bovine ELISA-PAGs (Pregnancy Associated Glycoproteins) kit was evaluated for 1) the diagnosis of pregnancy and 2) the determination of early pregnancy plasma profile of PAGs in Konya Merino ewes.
📊
Key Findings & Data:For these purposes, the blood samples were taken from 21 to 70 days of pregnancy by weekly.
💡
Conclusion & Impact:According to these results, it can be concluded that the commercial bovine ELISA-PAGs kit is good for detecting early pregnancy and monitoring fetoplacental unit well-being in Konya Merino ewes.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:: Erken gebelik · Konya Merinosu · Koyun

Abstract

In this study, the usability of bovine ELISA-PAGs (Pregnancy Associated Glycoproteins) kit was evaluated for 1) the diagnosis of pregnancy and 2) the determination of early pregnancy plasma profile of PAGs in Konya Merino ewes. For these purposes, the blood samples were taken from 21 to 70 days of pregnancy by weekly. According to the manufacturer’s instruction, the plasma level of PAGs was determined and accepted as the positive for pregnancy, if it was above than 0.3 OD (optimal density). An approximately linear increase in plasma profile of PAGs was determined for the period from 21 to 70 days of pregnancy. Percentages of the positive value of PAGs for on days 28, 35 and 42 were recorded as 30.8%, 69.2%, and 66.7%, respectively. According to these results, it can be concluded that the commercial bovine ELISA-PAGs kit is good for detecting early pregnancy and monitoring fetoplacental unit well-being in Konya Merino ewes. However, there is still a high margin of error for ewes if considering 0.3 OD for the positive.

Özet

Bu çalışmada, sığır ELISA-PAG (gebelik ilişkili glikoprotein) kitinin Konya Merinosu koyunlarda; 1) gebeliğin teşhisi ve 2) PAG'lerin erken gebelik plazma profilinin belirlenmesindeki kullanılabilirliği değerlendirildi. Bu amaçlar için, kan örnekleri gebeliğin 21-70. günleri arasında haftalık olarak alındı. Kit üreticisinin talimatına göre, PAG’lerin plazma seviyeleri belirlendi ve değer 0.3 OD’nin (optimal yoğunluk) üzerinde ise gebelik tanısı pozitif olarak kabul edildi. Gebeliğin 21 ve 70. günleri arasında PAG'lerin profilinde lineer benzeri bir artış olduğu belirlendi. PAG'lerin pozitif değer oranları 28, 35 ve 42. günlerde sırasıyla %30.8, %69.2 ve %66.7 olarak belirlendi. Bu sonuçlara göre, ticari sığır ELISA-PAG kitinin Konya Merinosu koyunlarda erken gebeliğin tespitinde ve fetoplasental yapının sağlığının izlenmesinde güvenilir olduğu belirlendi. Ancak, plazma örneklerinde gebelik tanısı için pozitif değer 0.3 OD kabul edilirse erken gebelikte yüksek bir hata payı ortaya çıkmaktadır.

🔖

Cite This Article

Kaplan, Y., Özyurtlu, N., Köse, M., Atlı, M. O., Küçükaslan, İ., Kırbaş, M. (2019). Detection of Plasma Profile of Pregnancy Associated Glycoproteins During Early Pregnancy in Pregnant Konya Merino Sheep. Veterinary Sciences and Practices, Cilt: 14 Sayı: 3, 307–314. https://doi.org/10.17094/ataunivbd.588666

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (31)

  1. Abbot P., Rokas A., 2017. Mammalian pregnancy. Current Biol, 27, 127-128
  2. Chavatte-Palmer P., Tarrade A., 2016. Placentation in different mammalian species. Ann Endocrinol, 77, 67-74
  3. Carter AM., Mess AM., 2017. The evolution of fetal membranes and placentation in carnivores and ungulates (Ferungulata). Anim Reprod, 14, 124-135
  4. Roberts RM., Green JA., Schulz LC., 2016. The Evolution of the Placenta. Reproduction, 152, 179-189
  5. Sammin D., Markey B., Bassett H., Buxton D., 2009. The ovine placenta and placentitis- A review. Vet Microbiol, 135, 90-97
  6. Igwebuike UM., 2009. A review of uterine structural modifications that influence conceptus implantation and development in sheep and goats. Anim Reprod Sci, 112, 1-7
  7. Szafranska B., Panasiewicz G., Majewska M., 2006. Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia)-a review. Reprod Nutr Dev, 46, 481-502
  8. Sousa NM., Ayad A., Beckers JF., Gajewski Z., 2006. Pregnancy-associated glycoproteins (PAG) as pregnancy markers in the ruminants. J Physiol Pharmacol, 57, 153-171
  9. Wallace RM., Pohler KG., Smith MF., Green JA., 2015. Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy. Reproduction, 149, 115-126
  10. Roberts JN., May KJ., Veiga-Lopez A., 2017. Time-dependent changes in pregnancy-associated glycoproteins and progesterone in commercial crossbred sheep. Theriogenology, 89, 271-279
  11. Resmi Gazete, 2017. 28.12.2017 tarih ve 26740 sayılı gazete, 2007/52 nolu tebliğ, Ek-34, Orta Anadolu Merinosu
  12. Kaymakçı M., Taşkın T., 2008. Türkiye koyunculuğunda melezleme çalışmaları. Hayvansal Üretim, 49, 43-51
  13. TAGEM, 2009. Türkiye Evcil Hayvan Genetik Kaynakları. 1. baskı, T.C. Tarım ve Köyişleri Bakanlığı, Tarımsal Araştırmalar Genel Müdürlüğü, Aralık 2009, Ankara
  14. Sousa NM., Garbayo JM., Figueiredo JR., Sulon J., Goncalves PB., Beckers JF., 1999. Pregnancy-associated glycoprotein and progesterone profiles during pregnancy and postpartum in native goats from the north-east of Brazil. Small Rumin Res, 32, 137-147
  15. El Amiri B., Karen A., Sulon J., Melo de Sousa N., Alvarez-Oxiley A., Cognie Y., Szenci O., Beckers JF., 2007. Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in Lacaune sheep. Reprod Dom Anim, 42, 257-262
  16. Spencer TE., Bazer FW., 2004. Conceptus signals for establishment and maintenance of pregnancy. Reprod Biol and Endocrinol, 2, 1-15
  17. Wallace JM., Da Silva P., Aitken RP., Cruickshank MA., 1997. Maternal endocrine status in relation to pregnancy outcome in rapidly growing adolescent sheep. J Endocrinol, 155, 359-368
  18. Johnson GA., Burghardt RC., Bazer FW., 2014. Osteopontin: a leading candidate adhesion molecule for implantation in pigs and sheep. J Anim Sci Biotechnol, 5, 1-15
  19. Imakawa K., Bai R., Fujiwara H., Kusama K., 2016. Conceptus implantation and placentation: molecules related to epithelial–mesenchymal transition, lymphocyte homing, endogenous retroviruses, and exosomes. Reprod Med Biol, 15, 1-11
  20. Uçar U., Köse M., Atlı MO., 2018. Konya Merinosu koyunlarda gebelik ilişkili glikoproteinlerin gebelikteki plazma profili ve erken gebelik tanısında kullanılabilirliği. Dicle Üniv Vet Fak Derg, 11, 77-82
  21. Balhara AK., Gupta M., Singh S., Mohanty AK., Singh I., 2013. Early pregnancy diagnosis in bovines: current status and future directions. Scientific World J, 5, 1-10
  22. Bazer FW., Spencer TE., Thatcher WW., 2012. Growth and development of the ovine conceptus. J Anim Sci, 90, 159-170
  23. Ehrhardt RA., Bell AW., 1995. Growth and metabolism of the ovine placenta during mid-gestation. Placenta, 16, 727-741
  24. Jones AK., Reed SA., 2017. Benefits of ultrasound scanning during gestation in the small ruminant. Small Rum Res, 149, 163-171
  25. Patel OV., Sulon J., Beckers JF., Takahashi T., Hirako M., Sasaki N., Domeki I., 1997. Plasma bovine pregnancy-associated glycoprotein concentrations throughout gestation in relationship to fetal number in the cow. Eur J Endocrinol, 137, 423-428
  26. Erdoğan G., 2012. Ultrasonic assessment during pregnancy in goats-A Review. Reprod Dom Anim, 47, 157-163
  27. Gonzalez de Bulnes A., Santiago Moreno J., Lopez Sebastian A., 1998. Estimation of foetal development in Manchega dairy ewes by transrectal ultrasonographic measurements. Small Rumin Res, 27, 243-250
  28. Gürler H., Kaymaz M., 2011. Akkaraman ırkı koyunlarda transrektal ve transabdominal muayene tekniği kullanılarak embriyonik ve fötal yaşın belirlenmesi. Ankara Üniv Vet Fak Derg, 58, 99-104
  29. Steckeler P., Weber F., Zerbe H., Rieger A., Voigt K., 2019. Evaluation of a bovine visual pregnancy test for the detection of pregnancy‐associated glycoproteins in sheep. Reprod Dom Anim, 54, 280-288
  30. Erdem H., Sarıbay MK., 2019. Gebelik ve tanı yöntemleri ‘‘Çiftlik Hayvanlarında Doğum ve Jinekoloji’’, Editörler: Mustafa Kaymaz, Murat Fındık, Ali Rişvanlı, Afşin Köker, 3. Baskı, 441-452, Medipres Matbaacılık Ltd. Şti, Malatya
  31. Thompson IM., Tao S., Branen J., Ealy AD., Dahl GE., 2013. Environmental regulation of pregnancy-associated glycoprotein B concentrations during late pregnancy in dairy cattle. J Anim Sci, 91, 168-173.