SavedAdd your journal

Journals / Dicle Üniversitesi Veteriner Fakültesi Dergisi / 2024 / Cilt: 17 Sayı: 2

The ısolation of mastitis agents and determination of sensitive antibiotics in goat milks with subclinical mastitis in Diyarbakır region

Diyarbakır bölgesinde subklinik mastitisli keçi sütlerinde mastitis etkenlerinin izolasyonu ve duyarlı antibiyotiklerin belirlenmesi

Simten Yeşilmen Alp(Dicle Üniversitesi Veteriner Fakültesi Klinik Öncesi Bilimler Bölümü Mikrobiyoloji Anabilim Dalı)0000-0002-2952-5180
Nihat Özyurtlu(DİCLE ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, VETERİNERLİK DOĞUM VE JİNEKOLOJİSİ ANABİLİM DALI)0000-0001-8115-2222
Feray Altan(DOKUZ EYLÜL ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, KLİNİK ÖNCESİ BİLİMLERİ BÖLÜMÜ, VETERİNERLİK FARMAKOLOJİ VE TOKSİKOLOJİSİ ANABİLİM DALI)0000-0002-9017-763X
Servet Bademkıran(DİCLE ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, VETERİNERLİK DOĞUM VE JİNEKOLOJİSİ ANABİLİM DALI)0000-0002-0466-8716
Aydın Vural(DİCLE ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, BESİN-GIDA HİJYENİ VE TEKNOLOJİSİ BÖLÜMÜ, VETERİNERLİK BESİN, GIDA HİJYENİ VE TEKNOLOJİSİ ANABİLİM DALI)0000-0002-6232-2131
Yasemin Kaplan Bilmez(GAP Uluslararası Tarımsal Araştırma ve Eğitim Merkezi)0000-0002-3071-4130
Pages
100–105
Publication Date
2024-12-31
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the MIC results against these bacteria isolated and identified in the study, it was determined that ciprofloxacin, tigecycline, benzylpenicillin, oxacillin and ceftazidimine could be effective in the treatment of mastitis.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:There has been an increase in the consumption of goat milk worldwide in recent years, as it is an alternative to cow milk for people with lactose intolerance.
📊
Key Findings & Data:Mastitis in goats negatively affects the quality of milk and leads to decreases in milk yield.
💡
Conclusion & Impact:According to the MIC results against these bacteria isolated and identified in the study, it was determined that ciprofloxacin, tigecycline, benzylpenicillin, oxacillin and ceftazidimine could be effective in the treatment of mastitis.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:İdentifikasyon · izolasyon · keçi

Abstract

There has been an increase in the consumption of goat milk worldwide in recent years, as it is an alternative to cow milk for people with lactose intolerance. Mastitis in goats negatively affects the quality of milk and leads to decreases in milk yield. Therefore, identifying mastitis pathogens is important to prevent infection. The aim of this study is to isolate and identify subclinical mastitis agents in goats in Diyarbakır and its districts and to determine effective antibiotics by the minimum inhibitory concentration (MIC) method. A total of 358 goat milk samples were examined for mastitis with the California Mastitis Test (CMT). In the microbiology examination of a total of 106 milk samples that were CMT (+2, +3) positive, while bacteria were isolated in 79 (74.5%) samples, no growth was observed in 27 (25.5%) samples.. Of the milk samples with bacterial growth, 30 (37.97%) were Staphylococcus aureus, 26 (32.91%) were Coagulase-negative Staphylococci (CNS), 3 (3.80%) were Streptococcus agalactiae, 1 (1.27%) Enterococcus spp., 18 (22.78%) Escherichia coli and 1 (1.27%) Acitenobacter spp. was isolated and identified. Proportional distribution of CNS positive bacteria: 20.25% Staphylococcus xylosus, 5.06% Staphylococcus warneri, 2.53% Staphylococcus lugdunensis, 1.27% Staphylococcus capitis, 2.53% Staphylococcus chromogenes and 1.27% Staphylococcus hominis were identified. According to the MIC results against these bacteria isolated and identified in the study, it was determined that ciprofloxacin, tigecycline, benzylpenicillin, oxacillin and ceftazidimine could be effective in the treatment of mastitis.

Özet

Keçi sütünün, laktoz intoleransı olan insanlarda inek sütüne alternatif olmasından dolayı son yıllarda dünya genelinde tüketiminde artışı görülmektedir. Keçi mastitisi, süt kalitesini olumsuz etkilemekte ve süt veriminde azalmalara yol açmaktadır. Bu nedenle mastitis patojenlerinin belirlenmesi enfeksiyonun önlenmesi açısından önemlidir. Bu çalışmanın amacı Diyarbakır ve ilçelerinde bulunan keçilerde, subklinik mastitis etkenlerinin izolasyonu ve identifikasyonu ile bunlara etkili antibiyotiklerin minimal inhibitör konsantrasyonu (MİK) yöntemi ile belirlenmesidir. Bu amaçla toplam 358 adet keçi süt örneğine California Mastitis Testi (CMT) ile mastitis yönünden bakıldı. CMT (+2,+3) pozitif olan toplam 106 süt örneğinin mikrobiyoloji incelemesinde 79’undan (%74.5) bakteri izole edilirken, 27’sinden (%25.5) herhangi bir üreme gözlenmedi. Üreme olan süt örneklerinin 30’undan (%37.97) Staphylococcus aureus ve 26’sından (%32.91) Koagülaz-negatif Staphylococcus (KNS), 3’ünden (%3.80) Streptococcus agalactiae, 1’inden (%1.27) Enterococcus spp., 18’inden (%22.78) Escherichia coli ve 1’inden de (%1.27) Acitenobacter spp. izole ve identifiye edildi. KNS pozitif olan bakterilerin orantısal olarak dağılımı %20.25 Staphylococcus xylosus, %5.06 Staphylococcus warneri, %2.53 Staphylococcus lugdunensis, %1.27 Staphylococcus capitis, %2.53 Staphylococcus chromogenes ve %1.27 Staphylococcus hominis olarak identifiye edildi. Çalışmada izole ve identifiye edilen bu bakterilere karşı yapılan MİK sonuçlarına göre siprofloksasin, tigesiklin, benzilpenisilin, oksasilin ve seftazidimin mastitis tedavisinde etkili olabileceği belirlendi.

🔖

Cite This Article

Alp, S. Y., Özyurtlu, N., Altan, F., Bademkıran, S., Vural, A., Bilmez, Y. K. (2024). The ısolation of mastitis agents and determination of sensitive antibiotics in goat milks with subclinical mastitis in Diyarbakır region. Dicle Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 17 Sayı: 2, 100–105. https://doi.org/10.47027/duvetfd.1477728

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (44)

  1. Fatmawati M, Suwanti LT, Mufasirin et al (2023). Epidemiological studies of subclinical mastitis in dairy goats in lumajang regency, east java, Indonesia. Jurnal Ilmu-Ilmu Peternakan., 33(3): 372-380.
  2. İlhan Z, Taşal İ, Sağcan S (2011). Subklinik mastitisli keçi sütlerinde aerobik bakterilerin izolasyonu. Yüzüncü Yıl Univ Vet Fak Derg., 22(2): 89-91.
  3. Cantekin Z, Özmen GÖ, Demir M, Yılmaz Er Z, Solmaz H, Ergün Y (2016). Detection of causative agents in goat mastitis and their antibiotic resistance in Hatay region. Van Vet J., 27(2): 79-83.
  4. Keskin D, Atay O, Kırkan Ş, Gökdal Ö, Tekbıyık S, Kaya O (2011). Detection of streptococcus agalactiae existence within milk samples of hair goats grown in west anatolia region. Agric J., 6(1): 31-34.
  5. Mishra AK, Shamra N, Singh DD et al (2018). Prevalence and bacterial etiology of subclinical mastitis in goats reared in organized farms. Vet World., 11(1): 20-24.
  6. Zhao Y, Liu H, Zhao X, Gao Y, Zhang M, Chen D (2015). Prevalence and pathogens of subclinical mastitis in dairy goats in China. Trop Anim Health Prod., 47: 429-435.
  7. McDougall S, Murdough P, Pankey W, Delaney C, Barlow J, Scruton D (2001). Relationships among somatic cell count, california mastitis test, ımpedance and bacteriological status of milk in goats and sheep in early lactation. Small Rumin Res., 40:245-254.
  8. Doğruer G, Sarıbay MK, Aslantaş Ö et al (2016). The prevalance, etiology and antimicrobial susceptibility of the microorganisms in subclinical mastitis in goats. Atatürk Üniversitesi Vet Bil Derg., 11(2):138-145.
  9. Monsang SW, Pal SK, Kumar M, Roy J, Sharma CS, Norjit SMN (2014). Bilateral mastectomy for successful management of chronic suppurative mastitis in a black bengal doe (Capra hircus). J Anim Health Prod., 2(2):28-30. doi.org/10.14737/journal.jahp/2014/2.2.28.30.
  10. Paape MJ, Poutrel B, Contreras A, Marco JC, Capuco AVM (2001). Milk somatic cells and lactation in small ruminants. J Dairy Sci,. 84(1):237-244. doi.org/10.3168/jds.S0022-0302(01)70223-8
  11. Pirzada M, Malhi KK, Kamboh AA et al (2016). Prevalence of subclinical mastitis in dairy goats caused by bacterial species. J Anim Health Prod. 4(2):55-59.
  12. Virdis S, Christian Scarano, Francesca Cossu, et al (2010). Antibiotic resistance in staphylococcus aureus and coagulase negative staphylococci ısolated from sar goats with subclinical mastitis. Vet Med Int., 111(6):404-412.
  13. Byeng RM, Grant T, Steve PH (2007). Effect of subclinical ıntramammary ınfection on somatic cell counts and chemical composition of goats’ milk. J Dairy Res., 74:204-210. doi.org/10.1017/S0022029906002378
  14. Bergonier DB, De Crémoux R, Rupp R, Lagriffoul G, Berthelot X (2003). Mastitis of dairy small ruminants. Vet Res., 34:689–716.
  15. Poutrel B.(1984). Udder ınfection of goats by coagulase-negative staphylococci. Vet Microbiol., 9:131-137.
  16. Da Silva ER, Siqueira AP, Martins JCD, Ferreira WPB, da Silva N (2004). Identification and ın vitro antimicrobial susceptibility of staphylococcus species ısolated from goat mastitis in the northeast of Brazil. Small Rumin Res., 55:45-49.
  17. McDougall S, Pankey W, Delaney C, Barlow J, Murdough PA, Scruton, D (2002). Prevalence and ıncidence of subclinical mastitis in goats and dairy ewes in Vermont, USA. Small Rumin Res., 46:115–121.
  18. Stuhr T, Aulrich K (2010). Intramammary ınfections in dairy goats: recent knowledge and ındicators for detection of subclinical mastitis. vTI Agric and Forestry Res., 60:267-279.
  19. Kumar V, Rana R, Mehra S, Rout PK (2013). Isolation and characterization of mycoplasma mycoides subspecies capri from milk of natural goat mastitis cases. ISRN Vet Sci., 2013:593029.
  20. Schalm OW, Carrol JE, Jain NC (1971). Bovine Mastitis 1st ed, Lea and Febiger, Philadelphia. pp:132-153
  21. Gebrewahid TT, Abera BH, Menghistu HT (2012). Prevalans and Etiology of Subclinical Mastitis in Small Ruminant of Tigray Regional State, North Ethiopia. Vet World. 5: 103-109.
  22. Shearer JK, Harris BJr (2003). Mastitis in Dairy Goats. DS 85 Animal Science University of Florida, Gainesville, USA.
  23. Aydın İ, Hadimli HH, Çelik HA, Sayın Z (2007). kıl keçilerinde subklinik mastitisin teşhisi için california mastitis test (CMT)’in değerlendirilmesi. Vet Bil Derg., 23(2):5-12.
  24. Markey B, Leonard F, Archambault M, Cullinane A, Maguire D (2013a). Section 36: Mastitis. In: Clinical Veterinary Microbiology. Edwards R, Hewat C (editors). 2th. ed. Missouri, USA: Mosby Elsevier. pp. 433-453.
  25. Markey B, Leonard F, Archambault M, Cullinane A, Maguire D (2013b). Section 35: The Mycoplasmos (class:Mollicutes). In: Clinical Veterinary Microbiology. Edwards R, Hewat C (editors). 2th. ed. Missouri, USA, Mosby Elsevier. pp. 423-431.
  26. Contreras A, Corrales JC, Sierra D (1995). Prevalence and aetiology of non-clinical ıntramammary ınfection in Murciano-Granadina goats. Small Rumin Res., 17:71-78.
  27. Moroni P, Pisoni G, Ruffo G, Boettcher PJ (2005a). Risk factors for ıntramammary ınfections and relationship with somatic-cell counts in Italian dairy goats. Prev Vet Med., 69:163-173.
  28. Kostelic A, Cergolj M, Tariba B et al (2009). Prevalence and aetiology of subclinical mastitis in goats. Ital J Anim Sci., 8:134-136.
  29. İşnel NB, Kırkan Ş (2012). Isolation of microorganisms from goats with subclinical mastitis and detection of antibiotics susceptibility. Animal Health Prod and Hyg., 1(2):106-112.
  30. Moroni P, Pisoni G, Antonini M, Ruffo G, Carli S, Varisco G, Boettcher P (2005b). Subclinical mastitis and antimicrobial susceptibility of Staphylococcus caprae and staphylococcus epidermidis ısolated from two Italian goat herds. J Dairy Sci,. 88:1694-1704.
  31. Bochev I, Russenova N (2005). Resistance of staphylococcus spp. strains ısolated from goats with subclinical mastitis. Bulg J Vet Med 8:109-118.
  32. Hossain MK, Paul S, HossainşM, Islam, MR, Alam MGS (2017). Bovine mastitis and ıts therapeutic strategy doing antibiotic sensitivity test. Austin J Vet Sci Anim Husb., 4(1):1030.
  33. Prestinaci F, Pezzotti P, Pantosti A. (2015). Antimicrobial resistance: a global multifaceted phenomenon. Pathog Glob Health., 109 (7):309– 318.
  34. Reller LB, Weinstein M, Jorgensen JH, Ferraro MJ (2009). Antimicrobial susceptibility testing: a review of general principles and contemporary practices. Clin Infect Dis., 49(11):1749-1755.
  35. Leekha S, Terrell CL, Edson, RS (2011). General principles of antimicrobial therapy. Mayo Clin Proc., 86(2):156-167
  36. Wiegand, I, Hilpert, K, Hancock, RE (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc., 3(2):163-175.
  37. Lo-Ten-Foe JR, de Smet AM, Diederen BM, Kluytmans JA, van Keulen PH (2007). Comparative evaluation of the Vitek 2, disk diffusion, etest, broth microdilution, and agar dilution susceptibility testing methods for colistin in clinical 39. ısolates, ıncluding heteroresistant Enterobacter cloacae and Acinetobacter baumannii strains. Antimicrob Agents Chemother., 51(10):3726-30.
  38. Bobenchik AM, Hindler JA, Giltner CL, Saeki S, Humphries RM (2014). Performance of Vitek 2 for antimicrobial susceptibility testing of Staphylococcus spp. and Enterococcus spp. J Clin Microbiol., 52(2):392-7.
  39. Nelli A, Voidarou C, Venardou B et al (2022). Antimicrobial and methicillin resistance pattern of potential mastitis-ınducing Staphylococcus aureus and coagulase-negative staphylococci ısolates from the mammary secretion of dairy goats. Biology., 11:1591.
  40. Kirecci E, Ergun Y, Dogruer G, Saribay MK (2009). Usefulness of the e-test for the determination of the susceptibility of Staphylococcus sp. ısolated from milk of sheep and goats with subclinical mastitis to amikacin and amoxicillin-clavulanic acid. Bull Vet Inst Pulawy., 53:401-405.
  41. Ariffin SMZ, Hasmadi N, Syawari NM et al (2019). Prevalence and antibiotic susceptibility pattern of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in dairy goats with clinical and subclinical mastitis. J Anim Health Prod., 7(1):32-37.
  42. Serrano‐Rodríguez JM, Cárceles‐García C, Cárceles‐Rodríguez CM, Gabarda ML, Serrano‐Caballero JM, Fernández‐Varón E (2017). susceptibility and PK/PD relationships of Staphylococcus aureus strains ısolated from the milk of sheep and goats with clinical mastitis to five veterinary fluoroquinolones. Vet Record., 180(15):376-376.
  43. Perez F, El Chakhtoura NG, Papp-Wallace KM, Wilson BM, Bonomo RA (2016). Treatment options for ınfections caused by carbapenem-resistant Enterobacteriaceae: can we apply “precision medicine” to antimicrobial chemotherapy?. Expert Opin Pharmacother., 17(6):761-781.
  44. Kim DH, Kim JH (2021). Efficacy of tigecycline and linezolid against pan-drug-resistant bacteria ısolated from companion dogs in south Korea. Front Vet Sci., 8:693506.