SavedAdd your journal

Journals / Veterinary Sciences and Practices / 2020 / Cilt: 15 Sayı: 3

The Effects of Ellagic Acid on Cecal Ligation and Puncture-Induced Lung Injury

Ellagik Asidin Çekal Ligasyon ve Delmeye Bağlı Akciğer Hasarı Üzerine Etkileri

Ersen Eraslan(BOZOK ÜNİVERSİTESİ)0000-0003-2424-2269
Derya Güzel(SAKARYA UNIVERSITY)
Songül Doğanay(SAKARYA UNIVERSITY)0000-0002-1730-1331
Mustafa Can Güler(ATATURK UNIVERSITY)
Ayhan Tanyeli(ATATURK UNIVERSITY)
Selim Çomaklı(ATATURK UNIVERSITY)
Pages
223–230
Publication Date
2020-12-31
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, Ellagic acid prevented CLP-induced lung injury in experimental rats.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Ellagic acid was examined on lung tissue injury caused by cecal ligation and puncture (CLP)-induced polymicrobial sepsis model in rats. 24 Wistar Albino female rats were allocated to 3 groups as: Group I (Sham), group II (CLP), and group III (CLP+ Ellagic acid 75 mg/kg).
📊
Key Findings & Data:Total oxidant status, tumor necrosis factor-α, interleukin-1β, malondialdehyde, and myeloperoxidase activity elevated, but superoxide dismutase and total antioxidant status valued declined in the CLP group compared to the sham group.
💡
Conclusion & Impact:In conclusion, Ellagic acid prevented CLP-induced lung injury in experimental rats.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Akciğer · Çekal ligasyon ve delinme · Ellagik asit

Abstract

Ellagic acid was examined on lung tissue injury caused by cecal ligation and puncture (CLP)-induced polymicrobial sepsis model in rats. 24 Wistar Albino female rats were allocated to 3 groups as: Group I (Sham), group II (CLP), and group III (CLP+ Ellagic acid 75 mg/kg). The CLP model was made by drilling the cecum. Biochemical and immunohistochemical analyzes were performed. Total oxidant status, tumor necrosis factor-α, interleukin-1β, malondialdehyde, and myeloperoxidase activity elevated, but superoxide dismutase and total antioxidant status valued declined in the CLP group compared to the sham group. On the contrary, superoxide dismutase and total antioxidant status levels increased. In contrast, myeloperoxidase activity, total oxidant status, tumor necrosis factor-α, interleukin-1β, and malondialdehyde levels decreased in the Ellagic acid treatment group. Caspase-3 and microtubule-associated protein light chain-3B immunopositivity increased significantly in the CLP group compared to the sham group while diminishing in the Ellagic acid group. In conclusion, Ellagic acid prevented CLP-induced lung injury in experimental rats. Thus, Ellagic acid may be an alternative therapeutic agent against lung tissue injury induced by sepsis.

Özet

Ellagik asit, sıçanlarda çekal ligasyon ve delmeye (ÇLD) bağlı polimikrobiyal sepsis modelinin neden olduğu akciğer dokusu hasarı üzerinde incelendi. 24 Wistar Albino dişi sıçan 3 gruba ayrıldı: Grup I (sham), grup II (ÇLD) ve grup III (ÇLD + Ellagik asit 75 mg / kg). ÇLD modeli çekumun delinmesi ile gerçekleştirildi. Biyokimyasal ve immünohistokimyasal analizler yapıldı. Total oksidan kapasite, tümör nekroz faktör-α, interlökin-1β, malondialdehit ve miyeloperoksidaz aktivitesi arttı, ancak süperoksit dismütaz ve total antioksidan kapasite değerleri sham grubuyla karşılaştırıldığında ÇLD grubunda azaldı. Aksine, ellagik asit tedavi grubunda miyeloperoksidaz aktivitesi, total oksidan kapasite, tümör nekroz faktör-α, interlökin-1β, ve malondialdehit seviyeleri azalırken süperoksit dismütaz ve total antioksidan kapasite düzeyleri artmıştır. Kaspaz-3 ve LC3B immünopozitifliği ellagik asit grubunda azalırken, ÇLD grubunda sham grubuna göre anlamlı olarak artmıştır. Sonuç olarak ellagik asit, deneysel sıçanlarda ÇLD'nin neden olduğu akciğer hasarını önledi. Dolayısıyla ellagik asit, sepsisin neden olduğu akciğer dokusu hasarına karşı alternatif bir terapötik ajan olabilir.

🔖

Cite This Article

Eraslan, E., Güzel, D., Doğanay, S., Güler, M. C., Tanyeli, A., Çomaklı, S. (2020). The Effects of Ellagic Acid on Cecal Ligation and Puncture-Induced Lung Injury. Veterinary Sciences and Practices, Cilt: 15 Sayı: 3, 223–230. https://doi.org/10.17094/ataunivbd.762960

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (47)

  1. Bhan C., Dipankar P., Chakraborty P., Sarangi PP., 2016. Role of cellular events in the pathophysiology of sepsis. Inflamm Res, 65, 853-68
  2. Mayr FB., Yende S., Angus DC., 2014. Epidemiology of severe sepsis. Virulence, 5, 4-11
  3. Angus DC., Wax RS., 2001. Epidemiology of sepsis: an update. Crit Care Med, 29, 109-116
  4. Troeger C., Blacker B., Khalil IA., Rao PC., Cao J., Zimsen SRM., Albertson SB., Deshpande A., Farag T., Abebe Z., Adetifa IMO., Adhikari TB., Akibu M., Al Lami FH., Al-Eyadhy A., Alvis-Guzman N., Amare AT., Amoako YA., Antonio CAT., Aremu O., Asfaw ET., Asgedom SW., Atey TM., Attia EF., Avokpaho EFGA., Ayele HT., Ayuk TB., Balakrishnan K., Barac A., Bassat Q., Behzadifar M., Behzadifar M., Bhaumik S., Bhutta ZA., Bijani A., Brauer M., Brown A., Camargos PAM., Castañeda-Orjuela CA., Colombara D., Conti S., Dadi AF., Dandona L., Dandona R., Phuc Do H., Dubljanin E., Edessa D., Elkout H., Endries AY., Fijabi DO., Foreman KJ., Forouzanfar MH., Fullman N., Garcia-Basteiro AL., Gessner BD., Gething PW., Gupta R., Gupta T., Hailu GB., Hassen HY., Hedayati MT., Heidari M., Hibstu DT., Horita N., Ilesanmi OS., Jakovljevic MB., Jamal AA., Kahsay A., Kasaeian A., Kassa DH., Khader YS., Khan EA., Khan MN., Khang YH, Kim YJ., Kissoon N., Knibbs LD, Kochhar S., Koul PA., Kumar GA, Lodha R., El Razek HMA., Malta DC., Mathew JL., Mengistu TD., Mezgebe HB., Mohammad KA., Mohammed MA., Momeniha F., Murthy S., Nguyen CT., Nielsen KR., Ningrum DNA., Nirayo YL., Oren E., Ortiz JR., Pa M., Postma MJ., Qorbani M., Quansah R., Rai RK., Rana SM., Ranabhat CL., Ray SE., Rezai MS., Ruhago GM., Safiri., Salomon JA., Sartorius B., Savic M., Sawhney M., She J., Sheikh A., Shiferaw MS., Shigematsu M., Singh JA., Somayaji R., Stanaway JD., Sufiyan MB., Taffere GR., Temsah MH., Thompson MJ., Tobe-Gai R., Topor-Madry R., Tran BX., Tran TT., Tuem KB., Ukwaja KN., Vollset SE., Walson JL, Weldegebreal F., Werdecker A., West TE., Yonemoto N., Zaki MS., Zhou L., Zodpey S., Vos W., Naghavi M, Lim SS., Mokdad AL., Murray CJL., Hay SI., Reiner Jr RC., 2016. Estimates of the global, regional, and national morbidity, mortality, and aetiologies of lower respiratory infections in 195 countries, 1990-2016: a systematic analysis for the Global Burden of Disease Study. Lancet Infect Dis, 18, 1191-210
  5. Chen X., Wang T., Song L., Liu X., 2019. Activation of multiple Toll-like receptors serves different roles in sepsis-induced acute lung injury. Exp Ther Med, 18, 443-450
  6. Zhou J., Fu Y., Liu K., Hou L., Zhang W., 2019. miR-206 regulates alveolar type II epithelial cell Cx43 expression in sepsis-induced acute lung injury. Exp Ther Med, 18, 296-304
  7. Störmann P., Becker N., Vollrath JT., Köhler K., Janicova A., Wutzler S., Hildebrand F., Marzi I., Relja B., 2019. Early local inhibition of Club Cell Protein 16 Following Chest Trauma Reduces Late Sepsis-Induced Acute Lung Injury. J Clin Med, 8, 896-901
  8. Güzel D., Tanyeli A., 2018. Inhibition of NADPH oxidase attenuates sepsis induced acute lung oxidative damage in rats. J Cell Neurosci Oxid Stress, 10, 714
  9. Tanyeli A., Güzel D., 2019. Alliin mitigates Cecal Ligation Puncture (CLP)-induced lung injury through antioxidant and antiinflammatory effects. TJOS, 4, 46-59
  10. Tanyeli A., Guzel D., 2019. Investigation into the Biochemical Effects of Barbaloin on Renal Tissue in Cecal Ligation and Puncture-Induced Polymicrobial Sepsis Model in Rats. South Clin Ist Euras, 30, 285-9
  11. Lelubre C., Vincent JL., 2018. Mechanisms and treatment of organ failure in sepsis. Nat Rev Nephrol, 14, 417-27
  12. Gotts JE., Matthay MA., 2016. Sepsis: pathophysiology and clinical management. BMJ, 353, 1585-2005
  13. Kaymak C., Basar H., Sardas S., 2011. Reactive oxygen species (ROS) generation in sepsis. FABAD J Pharm Sci, 36, 41-47
  14. De Jong HK., Van Der Poll T., Wiersinga WJ., 2010. The systemic pro-inflammatory response in sepsis. J Innate Immun, 2, 422-430
  15. Li K., Yang J., Han X., 2016. Ketamine attenuates sepsis-induced acute lung injury via regulationof HMGB1-RAGE pathways. Int Immunopharmacol, 34, 114-128
  16. Derosa G., Maffioli P., Sahebkar A., 2016. Ellagic acid and its role in chronic diseases. Adv Exp Med Biol, 928, 473-479
  17. Zafrilla P., Ferreres F., Tomas-Barberan FA., 2001. Effect of processing and storage on the antioxidant ellagic acid derivatives and flavonoids of red raspberry (Rubus idaeus) jams. J Agric Food Chem, 49, 3651-3655
  18. Seeram NP., Zhang Y., McKeever R., Henning SM., Lee RP., Suchard M., Li Z., Chen S., Thames G., Zerlin A., Nguyen M., Wang D., Dreher M., Heber D., 2008. Pomegranate juice and extracts provide similar levels of plasma and urinary ellagitannin metabolites in human subjects. J Med Food, 11, 390-394
  19. Salem AM., Mohammaden TF., Ali MAM., Mohamed EA., Hasan HF., 2016. Ellagic and ferulic acids alleviate gamma radiation and aluminium chloride-induced oxidative damage. Life Sci, 160, 2-11
  20. Masamune A., Satoh M., Kikuta K., Suzuki N., Satoh K., Shimosegawa T., 2005. Ellagic acid blocks activation of pancreatic stellate cells. Biochem Pharmacol, 70, 869-878
  21. Favarin CD., Teixeira MM., Lemos de Andrade E., de Freitas Alves C., Lazo Chica JE., Sorgi CA., Faccioli LH., Rogerio AP., 2013. Anti-inflammatory effects of ellagic acid on acute lung injury induced by acid in mice. Mediators Inflamm, 2013, 1-13
  22. Ekinci AFN., Tanyeli A., 2019. The antioxidant effect of fraxin against acute organ damage in polymicrobial sepsis model induced cecal ligation and puncture. TJOS, 4, 22-29
  23. Ohkawa H., Ohishi N., Yagi K., 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351-358
  24. Erel O., 2005. A new automated colorimetric method for measuring total oxidant status. Clin Biochem, 38, 1103-1111
  25. Bradley PP., Priebat DA., Christensen RD., Rothstein G., 1982. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol, 78, 206-209
  26. Sun Y., Oberley LW., Li Y., 1988. A simple method for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500
  27. Lo S., Yuan SS., Hsu C., Cheng YJ., Chang YF., Hsueh HW., Lee PH., Hsieh YC., 2013. Lc3 over-expression improves survival and attenuates lung injury through increasing autophagosomal clearance in septic mice. Ann Surg, 257, 352-363
  28. Rittirsch D., Flierl MA., Ward PA., 2008. Harmful molecular mechanisms in sepsis. Nat Rev Immunol, 8, 776-787
  29. Deutschman CS., Tracey KJ., 2014. Sepsis: current dogma and new perspectives. Immunity, 40, 463-475
  30. Napolitano LM., 2018. Sepsis 2018: Definitions and guideline changes. Surg Infect (Larchmt), 19, 117-125
  31. Ware LB., 2006. Pathophysiology of acute lung injury and the acute respiratory distress syndrome. Semin Respir Crit Care Med, 27, 337-349
  32. Polat B., Cadirci E., Halici Z., Bayir Y., Unal D., Bilgin BC., Yuksel TN., Vancelik S., 2013. The protective effect of amiodarone in lung tissue of cecal ligation and puncture-induced septic rats: a perspective from inflammatory cytokine release and oxidative stress. Naunyn Schmiedebergs Arch Pharmacol, 386, 635-643
  33. Butt Y., Kurdowska A., Allen TC., 2016. Acute lung injury: A clinical and molecular review. Arch Pathol Lab Med, 140, 345-350
  34. Akpinar E., Halici Z., Cadirci E., Bayir Y., Karakus E., Calik M., Topcu A., Polat B., 2014. What is the role of renin inhibition during rat septic conditions: preventive effect of aliskiren on sepsis-induced lung injury. Naunyn Schmiedebergs Arch Pharmacol, 387, 969-978
  35. Guo RF., Ward PA., 2007. Role of oxidants in lung injury during sepsis. Antioxid Redox Signal, 9, 1991-2002
  36. Macdonald J., Galley HF., Webster NR., 2003. Oxidative stress and gene expression in sepsis. Br J Anaesth, 90, 221-232
  37. Gough DR., Cotter TG., 2011. Hydrogen peroxide: a Jekyll and Hyde signalling molecule. Cell Death Dis, 2, 213-221
  38. Halliwell B., Aeschbach R., Löliger J., Aruoma OI., 1995. The characterization of antioxidants. Food Chem Toxicol, 33, 601-617
  39. Keith ES., Powers JJ., 1965. Effect of phenolic acids and esters on respiration and reproduction of bacteria in urine. Appl Microbiol, 13, 308-313
  40. Cadirci E., Altunkaynak BZ., Halici Z., Odabasoglu F., Uyanik MH., Gundogdu C., Suleyman G., Halici M., Albayrak M., Unal B., 2010. Alpha-lipoic acid as a potential target for the treatment of lung injury caused by cecal ligation and puncture-induced sepsis model in rats. Shock, 33, 479-484
  41. Akdis M., Aab A., Altunbulakli C., Azkur K., Costa RA., Crameri R., Duan S., Eiwegger T., Eljaszewicz A., Ferstl R., Frei R., Garbani M., Globinska A., Hess L., Huitema C., Kubo T., Komlosi Z., Konieczna P., Kovacs N., Kucuksezer UC., Meyer N., Morita H., Olzhausen J., O'Mahony L., Pezer M., Prati M., Rebane A., Rhyner C., Rinaldi A., Sokolowska M., Stanic B., Sugita K., Treis A., Veen W., Wanke K., Wawrzyniak M., Wawrzyniak P., Wirz OF., Zakzuk JS., Akdis CA., 2016. Interleukins (from IL-1 to IL-38), interferons, transforming growth factor β, and TNF-α: Receptors, functions, and roles in diseases. J Allergy Clin Immunol, 138, 984-1010
  42. Li JY., Wu HX., Yang G., 2017. Pachymic acid improves survival and attenuates acute lung injury in septic rats induced by cecal ligation and puncture. Eur Rev Med Pharmacol Sci, 21, 1904-1910
  43. Schulte W., Bernhagen J., Bucala R., 2013. Cytokines in sepsis: potent immunoregulators and potential therapeutic targets-an updated view. Mediators Inflamm, 2013, 1-16
  44. Sadasivan S., Waghray A., Larner SF., Dunn WA., Hayes RL., Wang KK., 2006. Amino acid starvation induced autophagic cell death in PC-12 cells: evidence for activation of caspase-3 but not calpain-1. Apoptosis, 11, 1573-1582
  45. Jiang Y., Gao M., Wang W., Lang Y., Tong Z, Kangkai Wang K., Zhang H., Chen G., Liu M., Yao Y., Xiao X., 2015. Sinomenine hydrochloride protects against polymicrobial sepsis via autophagy. Int J Mol Sci, 16, 2559-2573
  46. Wan SX., Shi B., Lou XL., Liu JQ., Ma GG., Liang DY., Ma S., 2016. Ghrelin protects small intestinal epithelium against sepsis-induced injury by enhancing the autophagy of intestinal epithelial cells. Biomed Pharmacother, 83, 1315-1320
  47. Sirois I., Groleau J., Pallet N., Brassard N., Hamelin K., Londono I., Pshezhetsky AV., Bendayan M., Hébert MJ., 2012. Caspase activation regulates the extracellular export of autophagic vacuoles. Autophagy, 8, 927-37