SavedAdd your journal

Journals / Gazi Sağlık Bilimleri Dergisi / 2023 / Cilt: 8 Sayı: 1

INTERFERENCE IN IMMUNOASSAYS

Eugene Gıtongorı MOGAKA
Resul KARAKUŞ
E. Ümit BAĞRIAÇIK
Pages
22–31
Publication Date
2023-03-24
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Insights for developing tests for assessing discordant results following troubleshooting of immunoassays as possible remedial measures have been provided.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Immunoassays are simple, efficient and reliable means for testing patient samples in the modern hospital.
📊
Key Findings & Data:Their specificity and sensitivity have provided robust means by which detection of analytes can be achieved with ease.
💡
Conclusion & Impact:Insights for developing tests for assessing discordant results following troubleshooting of immunoassays as possible remedial measures have been provided.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Endogenous · Exogenous · Immuno-assays

Abstract

Immunoassays are simple, efficient and reliable means for testing patient samples in the modern hospital. Their specificity and sensitivity have provided robust means by which detection of analytes can be achieved with ease. The underlying principle of antigen-antibody reactions forming a measurable complex exposes immunoassays to possible interference from proteins and other reactants with structural similarities to antibodies and or the analytes. Accounting for the sensitivity of the test and its reliability, the impact of interferents both endogenous like autoantibodies and exogenous factors like drugs, involved in the assay is worth investigating since the alternative would be producing unreliable results. Insights for developing tests for assessing discordant results following troubleshooting of immunoassays as possible remedial measures have been provided.

Özet

Immunoassays are simple, efficient and reliable means for testing patient samples in the modern hospital. Their specificity and sensitivity have provided robust means by which detection of analytes can be achieved with ease. The underlying principle of antigen-antibody reactions forming a measurable complex exposes immunoassays to possible interference from proteins and other reactants with structural similarities to antibodies and or the analytes. Accounting for the sensitivity of the test and its reliability, the impact of interferents both endogenous like autoantibodies and exogenous factors like drugs, involved in the assay is worth investigating since the alternative would be producing unreliable results. Insights for developing tests for assessing discordant results following troubleshooting of immunoassays as possible remedial measures have been provided.

🔖

Cite This Article

MOGAKA, E. G., KARAKUŞ, R., BAĞRIAÇIK, E. Ü. (2023). INTERFERENCE IN IMMUNOASSAYS. Gazi Sağlık Bilimleri Dergisi, Cilt: 8 Sayı: 1, 22–31. https://ajindex.com/articles/905805

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Wu AHB. A selected history and future of immunoassay development and applications in clinical chemistry. Clin Chim Acta [Internet] 2006 [cited 2021 Dec 15];369(2):119–24. Available from:
  2. Ruibal Morell A. Remembering our history: 60 years ago radioimmunoanalysis was discovered. Rev Española Med Nucl e Imagen Mol (English Ed 2020;39(6):337–9
  3. Luo YR, Chakraborty I, Lazar-Molnar E, Wu AHB, Lynch KL. Development of Label-Free Immunoassays as Novel Solutions for the Measurement of Monoclonal Antibody Drugs and Antidrug Antibodies. Clin Chem [Internet] 2020 [cited 2022 Jan 14];66(10):1319–28. Available from:
  4. Ahsan H. Monoplex and multiplex immunoassays: approval, advancements, and alternatives. Comp Clin Path [Internet] 2021 [cited 2022 Jan 14];1–13. Available from:
  5. Chigaev A, Sklar LA. Aspects of VLA-4 and LFA-1 regulation that may contribute to rolling and firm adhesion. Front Immunol [Internet] 2012 [cited 2020 Mar 3];3(AUG):242. Available from:
  6. Pan R, Li G, Liu S, Zhang X, Liu J, Su Z, et al. Emerging nanolabels-based immunoassays: Principle and applications in food safety. TrAC Trends Anal Chem 2021;145:116462
  7. Clinical and Laboratory Standards Institute. Interference Testing in Clinical Chemistry; Approved Guideline—Second Edition. [Internet]. Pennsylvania 19087-1898 USA, 2005: 2005 [cited 2021 Dec 20]. Available from:
  8. Ward G, Simpson A, Boscato L, Hickman PE. The investigation of interferences in immunoassay. Clin Biochem 2017;50(18):1306–11
  9. Juncker D, Bergeron S, Laforte V, Li H. Cross-reactivity in antibody microarrays and multiplexed sandwich assays: shedding light on the dark side of multiplexing. Curr Opin Chem Biol 2014;18(1):29–37
  10. Ghazal K, Brabant S, Prie D, Piketty ML. Hormone Immunoassay Interference: A 2021 Update. Ann Lab Med [Internet] 2022 [cited 2022 Jan 13];42(1):3–23. Available from:
  11. Chagoya Rodrigo C, Ruben Alejandro CP, Romero Javier H, Oviedo Cristobal L, Gutierrez Rosa Isela C, Medina Andrea V, et al. Hypersensivity Reactions to Steroids: Review. Glob Vaccines Immunol 2018;3(1)
  12. Emerson JF, Lai KKY. Endogenous Antibody Interferences in Imm