SavedAdd your journal

Journals / ADO Klinik Bilimler Dergisi / 2022 / Cilt: 11 Sayı: 2

Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer

Apoptoz ilişkili PDCD4: Oral kanserin erken tanısında umut vaadedici yeni bir biyobelirteç

Leyla BOZDAĞ
Leyla AÇIK
Halil ERSOY
Ömer BAYIR
Mehmet Hakan KORKMAZ
Nur MOLLAOĞLU
Sibel Elif GÜLTEKİN
Pages
150–156
Publication Date
2022-05-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study indicates that PDCD4 expression levels in liquid biopsies may have potential as a diagnostic biomarker in OSCC and OPMD.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim: This study aimed to investigate the potential role of the PDCD4 gene, involved in the progression of the oral potentially malign disorder (OPMD) and oral squamous cell carcinoma cancer (OSCC).
📊
Key Findings & Data:Material and Methods: The study was conducted on saliva and blood samples that were collected from OSCC (n=6), OPMD (n=6) patients, and healthy (n=6) individuals.
💡
Conclusion & Impact:This study indicates that PDCD4 expression levels in liquid biopsies may have potential as a diagnostic biomarker in OSCC and OPMD.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Oral skuamöz hücreli karsinom · Oral potansiyel malign bozukluk · PDCD4

Abstract

Aim: This study aimed to investigate the potential role of the PDCD4 gene, involved in the progression of the oral potentially malign disorder (OPMD) and oral squamous cell carcinoma cancer (OSCC). Material and Methods: The study was conducted on saliva and blood samples that were collected from OSCC (n=6), OPMD (n=6) patients, and healthy (n=6) individuals. The expression of PDCD4 was determined by using qRT-PCR. Kruskal Wallis test was performed to estimate the relationship between PDCD4 expression and clinical characteristics of OSCC and OPMD patients. The correlation between PDCD4 expression and patients groups was tested with the Rank Spearman test. Results: PDCD4 mRNA expression levels were assessed in 6 OSCCs, 6 OPMD patients, and 6 healthy individuals saliva and blood. In both blood and saliva, PDCD4 mRNA expression levels were 6/6 (100%) underexpressed in OSCC, while it was underexpressed in 5/6 (83%) of OPMD. The mean value of PDCD4 was significantly downregulated in OPMD and OSCC than in healthy (p<0.05). The Spearman rank test obtained a p-value for the 2 tail test of 0.024 (p < 0.05), which showed a significant correlation between both, while the correlation coefficient of -0.530 showed a strong negative correlation of PDCD4 downregülation and OPMD, OSCC. Conclusion: PDCD4 expression level is correlated with OSCC and OPMD. This study indicates that PDCD4 expression levels in liquid biopsies may have potential as a diagnostic biomarker in OSCC and OPMD.

Özet

Amaç: Bu çalışma, oral potansiyel malign bozukluk (OPMD) ve oral skuamöz hücreli karsinom karsinomun (OSCC) ilerlemesinde rol oynayan PDCD4 geninin potansiyel rolünü araştırmayı amaçlamıştır. Gereç ve Yöntem: Çalışma OSCC (n=6), OPMD (n=6) ve sağlıklı (n=6) bireylerden alınan tükürük ve kan örnekleri üzerinde gerçekleştirildi. PDCD4'ün ifadesi, qRT-PCR kullanılarak belirlendi. PDCD4 ekspresyonu ile OSCC ve OPMD hastalarının klinik özellikleri arasındaki ilişkiyi tahmin etmek için Kruskal Wallis testi yapıldı. PDCD4 ekspresyonu ile hasta gruplarının korelasyonu Rank Spearman testi ile test edildi. Bulgular: PDCD4 mRNA ekspresyon seviyeleri 6 OSCC'de, 6 OPMD hastasında ve 6 sağlıklı bireyde tükürük ve kanda değerlendirildi. Hem kanda hem de tükürükte, PDCD4 mRNA ekspresyon seviyeleri OSCC'de 6/6 (%100) düşük ifade edilirken, OPMD'nin 5/6'sında (%83) düşük ifade edildi. Ortalama PDCD4 değeri, OPMD ve OSCC'de sağlıklı olandan önemli ölçüde düşük ifade edildi (p<0.05). Rank Spearman testi ile p= 0.024 (p < 0.05) OSCC ve OPMD arasında anlamlı bir korelasyon gösterirken; -0.530 korelasyon katsayısı, , PDCD4 ‘ün OPMD ve OSCC arasında güçlü bir negatif korelasyona sahip olduğunu gösterdi. Sonuç: PDCD4 ekspresyon seviyesi, OSCC ve OPMD ile ilişkilidir. Bu çalışma, sıvı biyopsilerdeki PDCD4 ekspresyon seviyelerinin OSCC ve OPMD'de tanısal bir biyobelirteç olma potansiyeline sahip olabileceğini göstermektedir.

🔖

Cite This Article

BOZDAĞ, L., AÇIK, L., ERSOY, H., BAYIR, Ö., KORKMAZ, M. H., MOLLAOĞLU, N., GÜLTEKİN, S. E. (2022). Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer. ADO Klinik Bilimler Dergisi, Cilt: 11 Sayı: 2, 150–156. https://ajindex.com/articles/898707

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (9)

  1. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021;71(3):209-49.
  2. Madhura MG, Rao RS, Patil S, Fageeh HN, Alhazmi A, Awan KH. Advanced diagnostic aids for oral cancer. Dis Mon. 2020;66(12):101034. Wang J, Chang S, Li G, Sun Y. Application of liquid biopsy in precision medicine: opportunities and challenges. Front Med. 2017;11(4):522-7. van Ginkel JH, Huibers MMH, van Es RJJ, de Bree R, Willems SM. Droplet digital PCR for detection and quantification of circulating tumor DNA in plasma of head and neck cancer patients. BMC Cancer. 2017;17(1):428. Braig F, Voigtlaender M, Schieferdecker A, Busch CJ, Laban S, Grob T, et al. Liquid biopsy monitoring uncovers acquired RAS-mediated resistance to cetuximab in a substantial proportion of patients with head and neck squamous cell carcinoma. Oncotarget. 2016;7(28):42988-95.
  3. Perdomo S, Avogbe PH, Foll M, Abedi-Ardekani B, Facciolla VL, Anantharaman D, et al. Circulating tumor DNA detection in head and neck cancer: evaluation of two different detection approaches. Oncotarget. 2017;8(42):72621-32.
  4. Mazurek AM, Rutkowski T, Fiszer-Kierzkowska A, Malusecka E, Skladowski K. Assessment of the total cfDNA and HPV16/18 detection in plasma samples of head and neck squamous cell carcinoma patients. Oral Oncol. 2016;54:36-41.
  5. Guo PT, Yang D, Sun Z, Xu HM. PDCD4 functions as a suppressor for pT2a and pT2b stage gastric cancer. Oncol Rep. 2013;29(3):1007-12.
  6. Jajoo S, Mukherjea D, Kaur T, Sheehan KE, Sheth S, Borse V, et al. Essential role of NADPH oxidase-dependent reactive oxygen species generation in regulating microRNA-21 expression and function in prostate cancer. Antioxid Redox Signal. 2013;19(16):1863-76.
  7. Ma G, Zhang H, Dong M, Zheng X, Ozaki I, Matsuhashi S, et al. Downregulation of programmed cell death 4 (PDCD4) in tumorigenesis and progression of human digestive tract cancers. Tumour Biol. 2013;34(6):3879-85.
  8. Zhen Y, Li D, Li W, Yao W, Wu A, Huang J, et al. Reduced PDCD4 Expression Promotes Cell Growth Through PI3K/Akt Signaling in Non-Small Cell Lung Cancer. Oncol Res. 2016;23(1-2):61-8.
  9. Kujan O, Khattab A, Oliver RJ, Roberts SA, Thakker N, Sloan P. Why oral histopathology suffers inter-observer variability on grading oral epithelial dysplasia: an attempt to understand the sources of variation. Oral Oncol. 2007;43(3):224-31. Navazesh M. Methods for collecti