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Journals / Hittite Journal of Science and Engineering / 2023 / Cilt: 10 - Sayı: 1

Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine

Aykut Arif TOPÇU
Pages
77–82
Publication Date
2023-03-31
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the poly(hydroxyethyl methacrylate) [p(HEMA)] cryogel membrane with the assistance of polydopamine under alkaline conditions.
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Key Findings & Data:The maximum adsorption capacity (Qmax) of lysozyme was found as 108.9 mg/g and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model.
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Conclusion & Impact:Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:lysozyme · adsorption · molecular imprinting

Abstract

The surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the poly(hydroxyethyl methacrylate) [p(HEMA)] cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the artificial receptors, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and the swelling test were used for the characterization studies and the lysozyme adsorption capability of the adsorbent was evaluated in the aqueous solution. The maximum adsorption capacity (Qmax) of lysozyme was found as 108.9 mg/g and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model. The IF factor was 3.26 and the adsorbent was highly selective against creatinine and bovine serum albumin (BSA). Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.

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Cite This Article

TOPÇU, A. A. (2023). Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine. Hittite Journal of Science and Engineering, Cilt: 10 - Sayı: 1, 77–82. https://ajindex.com/articles/886356

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