Journals / Türk Veteriner Cerrahi Dergisi / 2022 / Cilt: 1 - Sayı: 1

Investigation of the Effects of St. John's Wort (Hypericum perforatum) Oil on Wound Healing in Mice

Farelerde Sarı Kantaron (Hypericum perforatum) Yağının Yara İyileşme Üzerine Etkilerinin Araştırılması

Pages
1–7
Publication Date
2022-03-30
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

John's wort oil does not have a negative effect on wound healing, also it does not speed up wound healing.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Although St.wort John's (Hypericum perforatum) oil is one of the most commonly used herbal products in traditional wound treatment, its effects should be examined further with controlled animal experiments.
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Key Findings & Data:Material and Methods: For this aim, 6-week-old male, 15 BALB / c rats were used.
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Conclusion & Impact:John's wort oil does not have a negative effect on wound healing, also it does not speed up wound healing.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Fare · Sarı Kantaron Yağı · Hypericum perforatum

Abstract

Objective: Although St.wort John's (Hypericum perforatum) oil is one of the most commonly used herbal products in traditional wound treatment, its effects should be examined further with controlled animal experiments. This study aimed to investigate the effects of St. John's wort oil on excisional full layer skin wound healing in mice. Material and Methods: For this aim, 6-week-old male, 15 BALB / c rats were used. The mice were divided into 3 groups as St. John's wort oil, olive oil, and physiological saline, each group of 5 mice. On the back skin of each mouse, six full-layered excisional skin wounds were created under anesthesia. The wound areas were photographed on a routine basis and the daily shrinkage of the wound area was observed with image processing techniques at three-day intervals in order to track and record the wound healing processes. On the 22nd day, wound tissues were collected and hydroxyproline and total protein levels were determined using an ELISA method. Results: The wound healing time, wound closure amount, hydroxyproline, and total protein levels between the groups were not statistically significant (p> 0.05). Conclusion: Based on the present study, St. John's wort oil does not have a negative effect on wound healing, also it does not speed up wound healing.

Özet

Amaç: Sarı kantaron otu (Hypericum perforatum) yağı geleneksel yara tedavisinde sıkça kullanılan bitkisel ürünlerden biri olsa da kontrollü hayvan deneyleri ile etkisinin daha ayrıntılı incelenmesi gerekmektedir. Çalışmada sarı kantaron yağının farelerde eksizyonel tam katmanlı deri yarası iyileşmesi üzerine etkilerinin araştırılması amaçlanmıştır. Materyal ve Metot: Bu amaçla 6 haftalık, erkek, 15 adet BALB/c ırkı fare kullanıldı. Fareler, her grupta 5’er adet fare olacak şekilde sarı kantaron yağı, zeytinyağı ve fizyolojik tuzlu su grubu olarak 3 gruba ayrıldı. Genel anestezi altında her farenin sırt derisine eksizyonel tam katmanlı 6 adet deri yarası açıldı. Yaraların iyileşme süreçlerinin takip ve kayıt edilebilmesi için yara bölgelerinden düzenli olarak fotoğraf alındı ve görüntü işleme teknikleri ile yara alanının günlük küçülmesi 3’er gün ara ile izlendi. İyileşen yara dokuları 22. günde ELISA yöntemi ile hidroksiprolin ve total protein düzeyleri ölçüldü. Bulgular: Elde edilen veriler ile yapılan istatistiksel çalışmada; gruplar arası yara iyileşme süresi, yara kapanma miktarı, hidroksiprolin ve total protein miktarları arasında istatistiksel olarak fark bulunmadı (p>0,05). Sonuç: Sonuçlar sarı kantaron otu yağının yara iyileşmesi üzerine olumsuz bir etkisinin olmadığını, ancak yara iyileşmesini hızlandırmadığına da işaret etmektedir.

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Cite This Article

AKDOĞAN, C., ÖZTURAN, Y. A., ÜNER, A. G., KALKAN, Y., AKIN, İ. (2022). Investigation of the Effects of St. John's Wort (Hypericum perforatum) Oil on Wound Healing in Mice. Türk Veteriner Cerrahi Dergisi, Cilt: 1 - Sayı: 1, 1–7. https://ajindex.com/articles/845570

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