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Journals / European Journal of Biology / 2020 / Cilt: 79 - Sayı: 2

Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity

Mustafa YILDIZ
Melike CELIK
Hakan TERZI
Pages
89–97
Publication Date
2020-12-25
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results: Our results indicated that SNP pre-treatment restored the seedling growth reduced by salinity stress.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The salinization of agricultural soils poses a serious challenge across the world.
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Key Findings & Data:Materials and Methods: To unravel the protective roles of exogenous SNP in alleviating salt-induced damage in barley (Hordeum vulgare L.), proteomic analysis was carried out on the leaves of seedlings exposed to 100 mM NaCl stress following 200 M SNP pre-treatment.
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Conclusion & Impact:Results: Our results indicated that SNP pre-treatment restored the seedling growth reduced by salinity stress.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Barley · Nitric oxide · Salinity stress

Abstract

Objective: The salinization of agricultural soils poses a serious challenge across the world. Although recent studies have shown that exogenous sodium nitroprusside (SNP) application can alleviate the harmful effects of salinity, the roles of SNP in the regulation of proteomic changes remain poorly understood. Materials and Methods: To unravel the protective roles of exogenous SNP in alleviating salt-induced damage in barley (Hordeum vulgare L.), proteomic analysis was carried out on the leaves of seedlings exposed to 100 mM NaCl stress following 200 M SNP pre-treatment. Results: Our results indicated that SNP pre-treatment restored the seedling growth reduced by salinity stress. Comparing 2-DE gels from the treatments showed that 24 proteins were differentially accumulated under SNP and/or NaCl stress treatments. Among them, 15 proteins were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Gene ontology analysis demonstrated that several pathways were regulated by SNP and/or NaCl treatments, including photosynthesis, protein metabolism, stress defense, and energy metabolism. Exogenous SNP increased the expression levels of 20 kDa chaperonin, proteasome subunit beta type-2, 2-Cys peroxiredoxin BAS1, ferredoxin-NADP reductase, thiazole biosynthetic enzyme 1-1, S-adenosylmethionine synthetase 3, and elongation factor Tu proteins in the leaves of barley seedlings under NaCl stress. Conclusion: Our results indicate that SNP pre-treatment may induce salinity tolerance growth inhibition through regulation of photosynthesis, activation of stress defence, degradation of damaged proteins, and the promoting of the synthesis of polyamines, proline, and GABA

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Cite This Article

YILDIZ, M., CELIK, M., TERZI, H. (2020). Proteomic Analysis of the Protective Effect of Sodium Nitroprusside on Leaves of Barley Stressed by Salinity. European Journal of Biology, Cilt: 79 - Sayı: 2, 89–97. https://ajindex.com/articles/82728

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References (13)

  1. Munns R, Tester M. Mechanisms of salinity tolerance. Ann Rev Plant Biol 2008; 59: 651–81
  2. Setia R, Gottschalk P, Smith P, Marschner P, Baldock J, Setia D et al., Soil salinity decreases global soil organic carbon stocks. Sci Total Environ 2013; 465: 267–72
  3. Soundararajan P, Manivannan A, Ko CH, Muneer S, Jeong BR. Leaf physiological and proteomic analysis to elucidate silicon induced adaptive response under salt stress in rosa hybrida ‘rock fire.’ Int J Mol Sci 2017; 18(8)
  4. Ahmad P, Abass AM, Nasser AM, Wijaya L, Alam P, Ashraf M. Mitigation of sodium chloride toxicity in Solanum lycopersicum L. by supplementation of jasmonic acid and nitric oxide. J Plant Interact 2018; 13(1): 64–72
  5. Hasanuzzaman M, Oku H, Nahar K, Bhuyan MHMB, Al Mahmud J, Baluska F et al., Nitric oxide-induced salt stress tolerance in plants: ROS metabolism, signaling, and molecular interactions. Plant Biotechnol Rep 2018; 12(2): 77–92
  6. Fancy NN, Bahlmann AK, Loake GJ. Nitric oxide function in plant abiotic stress. Plant Cell Environ 2017; 40(4): 462–72
  7. Ali Q, Daud MK, Haider MZ, Ali S, Rizwan M, Aslam N et al., Seed priming by sodium nitroprusside improves salt tolerance in wheat (Triticum aestivum L.) by enhancing physiological and biochemical parameters. Plant Physiol Biochem 2017; 119: 50–8
  8. Yadu S, Dewangan TL, Chandrakar V, Keshavkant S. Imperative roles of salicylic acid and nitric oxide in improving salinity tolerance in Pisum sativum L. Physiol Mol Biol Plants 2017; 23(1): 43–58
  9. Wu XX, Zhu XH, Chen JL, Yang SJ, Ding HD, Zha DS. Nitric oxide alleviates adverse salt-induced effects by improving the photosynthetic performance and increasing the anti-oxidant capacity of eggplant (Solanum melongena L.), J Hortic Sci Biotech 2015; 88(3): 352–60
  10. Fatma M, Khan NA. Nitric oxide protects photosynthetic capacity inhibition by salinity in Indian mustard. J Funct Environ Bot 2014; 4: 106–16
  11. Bai X, Yang L, Yang Y, Ahmad P, Yang Y, Hu X. Deciphering the protective role of nitric oxide against salt stress at the physiological and proteomic levels in maize. J Proteome Res 2011; 10: 4349–64
  12. Shen Z, Chen J, Ghoto K, Hu W, Gao G, Luo M et al., Proteomic analysis on mangrove plant Avicennia marina leaves reveals nitric oxide enhances the salt tolerance by up-regulating photosynthetic and energy metabolic protein expression, Tree Physiol 2018; 38 (11): 1605–22
  13. Ahsan N, Lee DG, Alam I, Kim PJ, Lee JJ, Ahn YO et al., Comparative proteomic study of arsen