As a result of the study, it is determined that highest the damage frequencies (%) as 69.00±4.58 and 66.00±3.00 were significantly observed in t0 and t6 groups respectively (P
📌 3 Key Takeaways
🎯
Objective & Hypothesis:In this study, DNA damage in hybrid tilapia exposed to the short-transport process has been investigated.
📊
Key Findings & Data:Gill samples were taken from tilapia which after immediately from the transport process (t0 group), after 6 hours from transport process (t6 group), after 12 hours from transport process (t12 group), after 24 hours from transport process (t24 group) and not applied transport process (control group) have been investigated and the results have been compared as statistically.
💡
Conclusion & Impact:As a result of the study, it is determined that highest the damage frequencies (%) as 69.00±4.58 and 66.00±3.00 were significantly observed in t0 and t6 groups respectively (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Tilapia · Fish transport · DNA damage
Abstract
In this study, DNA damage in hybrid tilapia exposed to the short-transport process has been investigated. Gill samples were taken from tilapia which after immediately from the transport process (t0 group), after 6 hours from transport process (t6 group), after 12 hours from transport process (t12 group), after 24 hours from transport process (t24 group) and not applied transport process (control group) have been investigated and the results have been compared as statistically. The Damage frequency (%), Arbitrary Unit and Genetic Damage Index (%) were evaluated in gill cells of tilapia. As a result of the study, it is determined that highest the damage frequencies (%) as 69.00±4.58 and 66.00±3.00 were significantly observed in t0 and t6 groups respectively (P
TURAN, F., ERGENLER, A. (2021). DNA Damage in Hybrid Tilapia (Oreochromis niloticus x O. aureus) Exposed to Short-Transport Process. Natural and Engineering Sciences, Cilt: 6 - Sayı: 3, 190–196. https://ajindex.com/articles/793138
Ahyat, N. M., Mohamad, F., Ahmad, A., & Azmi, A. A. (2017). Chitin and chitosan extraction from Portunus pelagicus. Malaysian Journal of Analytical Sciences, 21(4), 770-777.
Akdemir, G. (2019). Evaluation of rainbow trout (Oncorhynchus mykiss) gills as a biomarker in different live transfer applications (Master's thesis, Recep Tayyip Erdoğan University/Institute of Science, Rize, s 52. Barton, B.A. & Iwama, G.K. (1991). Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annual review of fish diseases1, 3–26.
Borreto, K.E., Valpato, G.L., & Pottinger, T.G. (2006). The Effect of Elevated Blood Cortisol Levels on The Extinction of a Conditioned Stress Response in Rainbow Trout. Hormones and Behavior 50: 484-488 pp. Chandroo, K.P., Cooke, S.J., Mckinley, R.S., & Moccia, R.D. (2005). Use of electromyogram telemetry to assess the behavioural and energetic responses of rainbow trout, Oncorhynchus mykiss (Walbaum) to transportation stress. Aquaculture Research, 36, 1226–1238.
Collins, A. (2004). The comet assay for DNA damage and repair. Molecular Biotechnology. 26,249–261.
Davies, K.J.A. (1995). Oxidative stress, the paradox of aerobic life. In Free Radical and Oxidative Stress: Environment, Drugs and Food Additives, 2nd ed.; Rice Evans, C., Halliwell, B., Land, G.G., Eds.; Portland Press:
London, UK, Volume 40, pp. 1–31.
Halliwell, B., & Gutteridge, J.M.C. (1999). Free Radicals in Biology and Medicine, 3rd ed.; Oxford University Press:
Oxford, UK; pp. 1–905.
Kamshilova, T.B., Mikryakoy, V.R. & Mikryakov, D.V. (2013). The effect of a cortisol analogue and transport stress on the frequency of occurrence of micronuclei in erythrocytes of the peripheral blood of sterlet Acipenser ruthenus L. Inland Water Biology, 6(2), 169-170.
Keleştemur, G.T. & Özdemir, Y. (2010). Effects of transport process on some blood parameter values of rainbow trout (Oncorhynchus mykiss, W. 1792). Suleyman Demirel University Faculty of Arts and Sciences Journal of Science, 5(2), 187-193. Norušis, M. J. (1993).
SPSS for Windows TM, Professional Statistics, Release 6.0. SPSS Incorporated Mayer, C., Popanda, O., Zelezny, O., von Brevern, M. C., Bach, A., Bartsch, H., & Schmezer, P. (2002). DNA repair capacity after γ-irradiation and expression profiles of DNA repair genes in resting and proliferating human peripheral blood lymphocytes. DNA Repair, 1(3), 237-250. OIE. (World Health Animal Organization) 2015. Welfare of Farmed