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Journals / Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries / 2025 / Cilt: 8 Sayı: 2

New Record and DNA Barcoding of the Flat Needlefish (Belonidae), Ablennes hians (Valenciennes, 1846) from Iskenderun Bay, Eastern Mediterranean, Türkiye

Türkiye'nin Doğu Akdeniz Kıyılarındaki İskenderun Körfezi'nde Bulunan Düz İğne Balığı (Belonidae), Ablennes hians (Valenciennes, 1846) Türünün Yeni Kayıt ve DNA Barkodlaması

Servet Ahmet Doğdu(Iskenderun Technical University, Maritime Technologies School of Higher Education, Underwater Technologies, Iskenderun, Hatay, Türkiye)0000-0003-2939-5838
Necdet Uygur(ISKENDERUN TECHNICAL UNIVERSITY)0000-0002-4460-1735
Cemal Turan(İSKENDERUN TEKNİK ÜNİVERSİTESİ)0000-0001-9584-0261
Funda Turan(İSKENDERUN TEKNİK ÜNİVERSİTESİ)0000-0002-0257-6009
Ali Uyan(İSKENDERUN TEKNİK ÜNİVERSİTESİ)0000-0001-9308-3324
Pages
148–159
Publication Date
2025-12-22
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Genetic distance results indicate that the Turkish population is extremely close to the Israel population (0.0085) this Mediterranean group is genetically distinct from the closest Red Sea (Egypt) population (0.0563) and other Indo-Pacific populations.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study reports the flat needlefish Ablennes hians on the coasts of Türkiye for only the second time. mtDNA COI gene region was used for molecular identification of the species.
📊
Key Findings & Data:On 21 December 2024, fishermen caught the specimens used in the study at a depth of 24 m in Iskenderun Bay.
💡
Conclusion & Impact:Genetic distance results indicate that the Turkish population is extremely close to the Israel population (0.0085) this Mediterranean group is genetically distinct from the closest Red Sea (Egypt) population (0.0563) and other Indo-Pacific populations.
🔬Design:Araştırma Makalesi
📍Scope:2024 Verileri
⚙️Variables:Belonidae · mtDNA COI · lesepsiyen göçü

Abstract

This study reports the flat needlefish Ablennes hians on the coasts of Türkiye for only the second time. mtDNA COI gene region was used for molecular identification of the species. On 21 December 2024, fishermen caught the specimens used in the study at a depth of 24 m in Iskenderun Bay. The COI sequences obtained in this study matched A. hians reference sequences with 100% identity in BLAST searches of the BOLD and NCBI databases. Genetic distance results indicate that the Turkish population is extremely close to the Israel population (0.0085) this Mediterranean group is genetically distinct from the closest Red Sea (Egypt) population (0.0563) and other Indo-Pacific populations. This verified that A. hians may have entered the Mediterranean via the Red Sea. On the other hand, more specimens from the Mediterranean, Red Sea and Atlantic Ocean should be studied to determine the true route of the species.

Özet

Bu çalışma Türkiye kıyılarında ikinci kez düz iğne balığı Ablennes hians'ın varlığını bildirmektedir. Türün moleküler tanımlanması için mtDNA COI gen bölgesi kullanılmıştır. Çalışmada kullanılan örnekler, 21 Aralık 2024 tarihinde İskenderun Körfezi'nde 24 m derinlikte balıkçılar tarafından yakalanmıştır. Bu çalışmada elde edilen COI dizileri, BOLD ve NCBI veritabanlarında yapılan BLAST aramalarında A. hians referans dizileriyle %100 özdeşlik göstermiştir. Genetik uzaklık sonuçları, Türkiye populasyonunun İsrail popülasyonuna (0.0085) son derece yakın olduğunu, bu Akdeniz grubunun, en yakın Kızıldeniz (Mısır) populasyonundan (0.0563) ve diğer Hint-Pasifik populasyonlarından genetik olarak ayrıştığını göstermektedir. Bu durum, A. hians'ın Kızıldeniz'den Akdeniz'e girmiş olabileceğini doğrulamaktadır. Ancak, türün gerçek rotasını belirlemek için Akdeniz, Kızıldeniz ve Atlantik Okyanusu'ndan daha fazla örneğin incelenmesi gerekmektedir.

🔖

Cite This Article

Doğdu, S. A., Uygur, N., Turan, C., Turan, F., Uyan, A. (2025). New Record and DNA Barcoding of the Flat Needlefish (Belonidae), Ablennes hians (Valenciennes, 1846) from Iskenderun Bay, Eastern Mediterranean, Türkiye. Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries, Cilt: 8 Sayı: 2, 148–159. https://doi.org/10.46384/jmsf.1719113

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References (63)

  1. Alshawy, F., Ibrahim, A., Hussein, C., & Lahlah, M. (2019). First record of the flat needlefish Ablennes hians (Valenciennes, 1846) from Syrian marine waters (eastern Mediterranean). Marine Biodiversity Records, 12, 1-4. doi:10.1186/s41200-019-0174-5
  2. Amir, S. A., Zhang, B., Masroor, R., Li, Y., Xue, D. X., Rashid, S.,. & Liu, J. (2022). Deeper in the blues: DNA barcoding of fishes from Pakistani coast of the Arabian Sea reveals overlooked genetic diversity. Marine Biodiversity, 52(4), 37. doi:10.1007/s12526-022-01272-6
  3. Asgharian, H., Sahafi, H. H., Ardalan, A. A., Shekarriz, S., & Elahi, E. (2011). Cytochrome c oxidase subunit 1 barcode data of fish of the Nayband National Park in the Persian Gulf and analysis using meta‐data flag several cryptic species. Molecular Ecology Resources, 11(3), 461-472. doi: 10.1111/j.1755-0998.2011.02989.x
  4. Azzurro, E., & D’Amen, M. (2022). Climate change paves the way for a new inter‐ocean fish interchange. Frontiers in Ecology and the Environment, 20(10), 558-563. doi:10.1002/fee.2459
  5. Bariche, M., Torres, M., Smith, C., Sayar, N., Azzurro, E., Baker, R., & Bernardi, G. (2015). Red Sea fishes in the Mediterranean Sea: a preliminary investigation of a biological invasion using DNA barcoding. Journal of Biogeography, 42(12), 2363-2373. doi:10.1111/jbi.12595
  6. Bemis, K. E., Girard, M. G., Santos, M. D., Carpenter, K. E., Deeds, J. R., Pitassy, D. E.,. & Williams, J. T. (2023). Biodiversity of Philippine marine fishes: A DNA barcode reference library based on voucher specimens. Scientific Data, 10(1), 411. doi:10.1038/s41597-023-02306-9
  7. Chang, C. H., Shao, K. T., Lin, H. Y., Chiu, Y. C., Lee, M. Y., Liu, S. H., & Lin, P. L. (2017). DNA barcodes of the native ray‐finned fishes in Taiwan. Molecular Ecology Resources, 17(4), 796-805.
  8. Collette, B. B., & Parin, N. V. (1970). Needlefishes Belonidae of the Eastern Atlantic Ocean. Atlantide. Danish Science Press.
  9. Collins, R. A., Boykin, L. M., Cruickshank, R. H., & Armstrong, K. F. (2012). Barcoding's next top model: an evaluation of nucleotide substitution models for specimen identification. Methods in Ecology and Evolution, 3(3), 457-465. doi:1010.1111/j.2041-210X.2011.00176.x
  10. Crocetta, F., Al Mabruk, S. A., Azzurro, E., Bakiu, R., Bariche, M., Batjakas, I. E.,. & Zenetos, A. (2021). New alien Mediterranean biodiversity records (November 2021). Mediterranean Marine Science, 22(3): 724-746. doi:10.12681/mms26668
  11. Deidun, A., Zava, B., Corsini-Foka, M., Galdies, J., Natale, A. D., & Collette, B. B. (2021). First record of the flat needlefish Ablennes hians (Belonidae) in central Mediterranean waters (western Ionian Sea). Annales 31(1):9-16.
  12. Deidun, A., Corsini-Foka, M., Marrone, A., Galdies, J., Zava, B., Crobe, V., & Tinti, F. (2024). Yet another non-indigenous fish from Maltese waters, central Mediterranean: a first record of Lagocephalus guentheri Miranda Ribeiro, 1915 (Tetraodontiformes, Tetraodontidae). BioInvasions Records, 13(3), 777–786, doi:10.3391/bir.2024.13.3.16
  13. Doğdu, S. A., & Turan, C. (2024). Biological and growth parameters of Plotosus lineatus in the Mediterranean Sea. PeerJ, 12, e16945. doi:10.7717/peerj.16945
  14. Fricke R, Eschmeyer WN, Van der Laan R (eds) (2025) Eschmeyer's Catalog of Fishes: Genera, Species, References. Electronic version accessed 12 Feb 2025.
  15. Froese, R. & D. Pauly. Editors. (2025). FishBase. World Wide Web electronic publication. www.fishbase.org, version (04/2025). Retrieved from https://fishbase.se/
  16. Galil, B. S., Marchini, A., & Occhipinti-Ambrogi, A. (2018). Mare Nostrum, mare quod invaditur—the history of bioinvasions in the Mediterranean Sea. Histories of Bioinvasions in the Mediterranean, 21-49. doi:10.1007/978-3-319-74986-0_2
  17. Golani, D. (2019). First record of the flat needlefish Ablennes hians (Valenciennes, 1846) in the Mediterranean Sea (Osteichthyes, Beloniformes, Belonidae). BioInvasions Records, 8(2), 410-412. doi:10.3391/bir.2019.8.2.22
  18. Golani, D., Azzurro, E., Dulčić, J., Massutí, E., & Orsi-Relini, L. (2021). Atlas of exotic fishes in the Mediterranean Sea. CIESM PUBLISHERS/Paris, Monaco.
  19. Golani, D. (2025). Update of Red Sea (Lessepsian) fish species in the Mediterranean Sea since the 2nd CIESM Atlas of Exotic Fish. Mediterranean Marine Science, 26(1), 149-155. doi:10.12681/mms.39390
  20. Hall, T. (2011). BioEdit: an important software for molecular biology. GERF Bulletin of Biosciences, 2(1), 60-61.
  21. Habib, K. A., Islam, M. J., Sakib, M. N., Brishti, P. S., & Neogi, A. K. (2023). DNA barcoding of reef‐associated fishes of Saint Martin's Island, Northern Bay of Bengal, Bangladesh. Ecology and Evolution, 13(10), e10641.
  22. Hebert, P. D., Ratnasingham, S., & De Waard, J. R. (2003). Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society of London. Series B: Biological Sciences, 270(1), 96-99. doi: 10.1098/rsbl.2003.0025
  23. Irmak, E., & Özden, U. (2023). Expansion of the flat needlefish Ablennes hians (Valenciennes, 1846) distribution in the eastern Mediterranean. Acta Adriatica, 64(1), 91-92. doi:10.32582/aa.64.1.6
  24. Karahan, A., Douek, J., Paz, G., Stern, N., Kideys, A. E., Shaish, L.,. & Rinkevich, B. (2017). Employing DNA barcoding as taxonomy and conservation tools for fish species censuses at the southeastern Mediterranean, a hot-spot area for biological invasion. Journal for Nature Conservation, 36, 1-9. doi:10.1016/j.jnc.2017.01.004
  25. Karan, S., Uyan, A., Doğdu, S. A., Gürlek, M., Ergüden, D., & Turan, C. (2019). Genetic confirmation of Red cornetfish, Fistularia petimba (Syngnathiformes: Fistularidae) occurrence in Turkish marine waters. FishTaxa-Journal of Fish Taxonomy, 4(3).
  26. Karataş, A., Filiz, H., Erciyas-Yavuz, K., Özeren, S. C., & Tok, C. V. (2021). The vertebrate biodiversity of Turkey. In Biodiversity, Conservation and Sustainability in Asia: Volume 1: Prospects and Challenges in West Asia and Caucasus (pp. 175-274). Cham: Springer International Publishing.
  27. Khalil, M. T., Mostafa, A. B., & El-Naggar, M. M. (2025). Climate Change and Lessepsian Migration to the Mediterranean Sea. In Climate Changes Impacts on Aquatic Environment: Assessment, Adaptation, Mitigation, and Road Map for Sustainable Development (pp. 85-118). Cham: Springer Nature Switzerland. doi:10.1007/978-3-031-74897-4_4
  28. Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16(2), 111–120. doi:10.1007/BF01731581
  29. Kumar, S., Stecher, G., Suleski, M., Sanderford, M., Sharma, S., & Tamura, K. (2024). MEGA12: Molecular Evolutionary Genetic Analysis version 12 for adaptive and green computing. Molecular Biology and Evolution, 41(12), 1-9. doi:10.1093/molbev/msae263
  30. Landi, M., Dimech, M., Arculeo, M., Biondo, G., Martins, R., Carneiro, M.,. & Costa, F. O. (2014). DNA barcoding for species assignment: the case of Mediterranean marine fishes. PLoS One, 9(9), e106135. doi:10.1371/journal.pone.0106135
  31. Langeneck, J., Bakiu, R., Chalari, N., Chatzigeorgiou, G., Crocetta, F., Doğdu, S. A.,. & Zenetos, A. (2023). New records of introduced species in the Mediterranean Sea (November 2023). Mediterranean Marine Science, 24(3), 610-632. doi:10.12681/mms.35840
  32. Mutlu, E., Meo, I. D., Miglietta, C., & Deval, M. C. (2023). Ecological indicative stressors of native vs. non-native fish in an ultra-oligotrophic region of the Mediterranean Sea. Sustainability, 15(3), 2726. doi:10.3390/su15032726
  33. Ragkousis, M., Zenetos, A., Souissi, J., Hoffman, R., Ghanem, R., TAŞKIN, E.,. & Kousteni, V. (2023). Unpublished Mediterranean and Black Sea records of marine alien, cryptogenic, and neonative species. BioInvasions Records, 12(2): 339–369. doi:10.3391/bir.2023.12.2.01
  34. Ribeiro, A. D. O., Caires, R. A., Mariguela, T. C., Pereira, L. H. G., Hanner, R., & Oliveira, C. (2012). DNA barcodes identify marine fishes of São Paulo State, Brazil. Molecular Ecology Resources, 12(6), 1012-1020. doi:10.1111/1755-0998.12007
  35. Sanger, F., Nicklen, S., & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, 74(12), 5463-5467. doi:10.1073/pnas.74.12.5463.
  36. Segura-Garcia, I., & Yain Tun, T. (2018). Unscrambling Myanmar marine fishery biodiversity using DNA barcoding. Unpublished. Submitted to the EMBL/GenBank/DDBJ databases.
  37. Steinke, D., Zemlak, T. S., Connell, A. D., Heemstra, P. C., & Hebert, P. D. N. (2011). DNA Barcodes: Linking adults and immatures of marine fishes. Unpublished. Submitted to the EMBL/GenBank/DDBJ databases.
  38. Sliskovic, M., Božić, K., Žanić Mikuličić, J., & Kolanović, I. (2024). Addressing the Significance of the Union List with a Focus on Marine Invasive Alien Species Impacts. Sustainability, 16(21), 9435. doi:10.3390/su16219435
  39. Tadmor-Levi, R., David, L., & Golani, D. (2020). Indication of cryptic taxa within the flat needlefish, Ablennes hians based on analysis of the cytochrome oxidase I region on a wide range of samples. Marine Biology Research, 16(6-7), 474-479. doi:10.1080/17451000.2020.1834117
  40. Toji, T., Abdussamad, E. M., Ameri, S., Sijad, B., & Sajikumar, K. K. (2024). An integrative taxonomic study on needlefishes (Belonidae) unveils two new species within the genus Ablennes from the Indian Ocean. Regional Studies in Marine Science, 74, 103522. doi:10.1016/j.rsma.2024.103522
  41. Thu, P. T., Huang, W. C., Chou, T. K., Van Quan, N., Van Chien, P., Li, F.,. & Liao, T. Y. (2019). DNA barcoding of coastal ray-finned fishes in Vietnam. PloS One, 14(9), e0222631. doi:10.1371/journal.pone.0222631
  42. Turan, C., Ergüden, D., & Gürlek, M. (2016). Climate change and biodiversity effects in Turkish Seas. Natural and Engineering Sciences, 1(2), 15-24. doi:10.28978/nesciences.286240
  43. Turan, C., Gürlek, M., Ergüden, D., Uyan, A., Karan, S., & Doğdu, S. A. (2017). Assessing DNA barcodes for identification of pufferfish species (Tetraodontidae) in Turkish marine waters. Natural and Engineering Sciences, 2(3), 55-66. doi:10.28978/nesciences.369538
  44. Turan, C., Gürlek, M., Başusta, N., Uyan, A., Doğdu, S. A., & Karan, S. (2018). A checklist of the non-indigenous fishes in Turkish marine waters. Natural and Engineering Sciences, 3(3), 333-358. doi:10.28978/nesciences.468995
  45. Turan, C., Uyan, A., Gürlek, M., & Doğdu, S. A. (2020). DNA barcodes for identifications of two lionfish species Pterois miles (Bennett, 1828) and P. volitans (Linnaeus, 1758) in the Mediterranean. FishTaxa-Journal of Fish Taxonomy, 16: 29-36
  46. Turan, C., & Doğdu, S. A. (2022). Preliminary assessment of invasive lionfish Pterois miles using underwater visual census method in the Northeastern Mediterranean. Croatian Journal of Fisheries: Ribarstvo, 80(1), 38-46. doi:10.2478cjf-2022-0005
  47. Turan, C., Yağlıoğlu, D., Doğdu, S. A., Ergüden, D., Ivanova, P. P., & Raykov, V. S. (2023). Existence of Belone svetovidovi Collette & Parin, 1970 (Family: Belonidae) in the Marmara Sea and Black Sea Coasts of Türkiye. Natural and Engineering Sciences, 8(2), 72-81. doi:10.28978/nesciences.1331296
  48. Turan, C., Ergüden, D., Gürlek, M., & Doğdu, S. A. (2024). Checklist of alien fish species in the Turkish marine ichthyofauna for science and policy support. Tethys Environmental Science, 1(2), 50-86. doi:10.5281/zenodo.12771318
  49. Turan, C., Ergenler, A., Turan, F., & Doğdu, S. A. (2024). Genetic Confirmation of the Striped Eel Catfish Plotosus lineatus (Thunberg, 1787) from Iskenderun Bay (Eastern Mediterranean, Türkiye). Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries, 7(1), 24-29. doi:10.46384/jmsf.1449570
  50. Turan, C., Ivanova, P. P., Doğdu, S. A., Ergüden, D., Raykov, V. S., Yankova, M.,. & Ergenler, A. (2025). Systematic identification of needlefish (Belonidae) species using molecular genetic and morphological markers in the Mediterranean and Black Seas. PloS One, 20(2), e0315401. doi:10.1371/journal.pone.0315401 (In Press).
  51. Yağlıoğlu, D., Doğdu, S. A., & Turan, C. (2023). First morphological and genetic record and confirmation of Korean rockfish Sebastes schlegelii Hilgendorf, 1880 in the Black Sea Coast of Türkiye. Natural and Engineering Sciences, 8(3), 140-150. doi:10.28978/nesciences.1363941
  52. Zhang, J. B., & Hanner, R. (2011). DNA barcoding is a useful tool for the identification of marine fishes from Japan. Biochemical Systematics and Ecology, 39(1), 31-42.
  53. Zenetos, A., Doğan, A., Bakir, A. K., Chatzigeorgiou, G., Corsini-Foka, M., Dağli, E., & Galanidi, M. (2024). Non-Indigenous Species (NIS) Know No Geopolitical Borders—An Update of NIS in the Aegean Sea. Diversity, 17(1), 12. doi:10.3390/d17010012
  54. Ward, R. D., Zemlak, T. S., Innes, B. H., Last, P. R., & Hebert, P. D. (2005). DNA barcoding Australia's fish species. Philosophical Transactions of the Royal Society B: Biological Sciences, 360(1462), 1847-1857. doi:10.1098/rstb.2005.1716.
  55. Amir, S. A., Zhang, B., Masroor, R., Li, Y., Xue, D. X., Rashid, S.,. & Liu, J. (2022). Deeper in the blues: DNA barcoding of fishes from Pakistani coast of the Arabian Sea reveals overlooked genetic diversity. Marine Biodiversity, 52(4), 37. doi:10.1007/s12526-022-01272-6
  56. Bemis, K. E., Girard, M. G., Santos, M. D., Carpenter, K. E., Deeds, J. R., Pitassy, D. E.,. & Williams, J. T. (2023). Biodiversity of Philippine marine fishes: A DNA barcode reference library based on voucher specimens. Scientific Data, 10(1), 411. doi:10.1038/s41597-023-02306-9
  57. Crocetta, F., Al Mabruk, S. A., Azzurro, E., Bakiu, R., Bariche, M., Batjakas, I. E.,. & Zenetos, A. (2021). New alien Mediterranean biodiversity records (November 2021). Mediterranean Marine Science, 22(3): 724-746. doi:10.12681/mms26668
  58. Karahan, A., Douek, J., Paz, G., Stern, N., Kideys, A. E., Shaish, L.,. & Rinkevich, B. (2017). Employing DNA barcoding as taxonomy and conservation tools for fish species censuses at the southeastern Mediterranean, a hot-spot area for biological invasion. Journal for Nature Conservation, 36, 1-9. doi:10.1016/j.jnc.2017.01.004
  59. Landi, M., Dimech, M., Arculeo, M., Biondo, G., Martins, R., Carneiro, M.,. & Costa, F. O. (2014). DNA barcoding for species assignment: the case of Mediterranean marine fishes. PLoS One, 9(9), e106135. doi:10.1371/journal.pone.0106135
  60. Langeneck, J., Bakiu, R., Chalari, N., Chatzigeorgiou, G., Crocetta, F., Doğdu, S. A.,. & Zenetos, A. (2023). New records of introduced species in the Mediterranean Sea (November 2023). Mediterranean Marine Science, 24(3), 610-632. doi:10.12681/mms.35840
  61. Ragkousis, M., Zenetos, A., Souissi, J., Hoffman, R., Ghanem, R., TAŞKIN, E.,. & Kousteni, V. (2023). Unpublished Mediterranean and Black Sea records of marine alien, cryptogenic, and neonative species. BioInvasions Records, 12(2): 339–369. doi:10.3391/bir.2023.12.2.01
  62. Thu, P. T., Huang, W. C., Chou, T. K., Van Quan, N., Van Chien, P., Li, F.,. & Liao, T. Y. (2019). DNA barcoding of coastal ray-finned fishes in Vietnam. PloS One, 14(9), e0222631. doi:10.1371/journal.pone.0222631
  63. Turan, C., Ivanova, P. P., Doğdu, S. A., Ergüden, D., Raykov, V. S., Yankova, M.,. & Ergenler, A. (2025). Systematic identification of needlefish (Belonidae) species using molecular genetic and morphological markers in the Mediterranean and Black Seas. PloS One, 20(2), e0315401. doi:10.1371/journal.pone.0315401 (In Press).