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Journals / Natural and Engineering Sciences / 2022 / Cilt: 7 - Sayı: 2

Monitoring DNA Damage in Suez Pufferfish (Lagocephalus suezensis) from the Northeastern Mediterranean

Funda TURAN
Ayşegül ERGENLER
Fevzi BARDAKÇI
Pages
190–199
Publication Date
2022-08-08
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Statistically significant differences were found between DNA damage, Arbitrary unit and genetic damage index values of the two locations (p

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Industrial effluents, agricultural runoff, and municipal wastewaters contain unknown substances and complex mixtures that are released into the environment and can lead to contamination of surface and subsurface waters.
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Key Findings & Data:The highest level of DNA damage (55.01±1.02%), arbitrary unit (143.01±7.21) and genetic damage index (1.43±0.07 %) were found in gill cells of L. suezensis from Mersin Bay.
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Conclusion & Impact:Statistically significant differences were found between DNA damage, Arbitrary unit and genetic damage index values of the two locations (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Lagocephalus suezensis · DNA damage · comet assay

Abstract

Industrial effluents, agricultural runoff, and municipal wastewaters contain unknown substances and complex mixtures that are released into the environment and can lead to contamination of surface and subsurface waters. Such activities have endangered the existence of ecosystems and their inhabitants. Changes in the genome caused by genotoxic agents led to mutations and pose a burden to the populations of fish species. In the present study, we have used the alkaline Comet assay to detect the genotoxicity in Suez pufferfish (Lagocephalus suezensis) sampled from two different gulfs (Iskenderun and Mersin Bay), Northeastern Mediterranean. At the end of the study, the damage frequency (%), arbitrary unit and genetic damage index (%) were evaluated in gill and liver cells of L. suezensis by comet assay. The DNA damage in the gill and liver cells of L. suezensis in the present study were observed with a higher level of DNA damage in gill cells compared to liver cells in both the Iskenderun and Mersin Bays. The highest level of DNA damage (55.01±1.02%), arbitrary unit (143.01±7.21) and genetic damage index (1.43±0.07 %) were found in gill cells of L. suezensis from Mersin Bay. Statistically significant differences were found between DNA damage, Arbitrary unit and genetic damage index values of the two locations (p

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Cite This Article

TURAN, F., ERGENLER, A., BARDAKÇI, F. (2022). Monitoring DNA Damage in Suez Pufferfish (Lagocephalus suezensis) from the Northeastern Mediterranean. Natural and Engineering Sciences, Cilt: 7 - Sayı: 2, 190–199. https://ajindex.com/articles/793123

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References (11)

  1. Ameur, W. B., de Lapuente, J., El Megdiche, Y., Barhoumi, B., Trabelsi, S., Camps, L., & Borràs, M. (2012). Oxidative stress, genotoxicity and histopathology biomarker responses in mullet (Mugil cephalus) and sea bass (Dicentrarchus labrax) liver from Bizerte Lagoon (Tunisia). Marine Pollution Bulletin, 64(2), 241-251. https://doi.org/10.1016/j.marpolbul.2011.11.026.
  2. Ateeq, B., Farah, M. A., & Ahmad, W. (2005). Detection of DNA damage by alkaline single cell gel electrophoresis in 2, 4-dichlorophenoxyacetic-acid-and butachlor-exposed erythrocytes of Clarias batrachus. Ecotoxicology and Environmental Safety, 62(3), 348-354.
  3. Anderson, M.R., Scruton, D.A., Williams, U.P., Payne, J.F. (1995). Mercury in fish in the Smallwood Reservoir, Labrador, twenty one years after impoundment. Water Air Soil Pollution, 80(1–4):927–930. https://doi.org/10.1007/BF01189746.
  4. Bolognesi, C., Cirillo, S., & Chipman, J. K. (2019). Comet assay in ecogenotoxicology: Applications in Mytilus sp. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 842, 50-59. https://doi.org/10.1016/j.mrgentox.2019.05.004.
  5. Cavalcante, D.G.S.M., Martinez, C.B.R., Sofia, S.H. (2008). Genotoxic effects of roundup on the fish Prochilodus lineatus. Mutation Research, 655,41–46.
  6. Collins, A., 2004. The comet assay for DNA damage and repair. Molecular Biotechnology 26, 249–261. https://doi.org/10.1385/MB:26:3:249.
  7. Cui, Z., Luan, X., Li, S., Zhao, X., Lin, Z., Li, J.,. & Xie, J. (2021). Genotoxicity detection of oil-containing drill cuttings by Comet assay based on a demersal marine fish Mugilogobius chulae. Ecotoxicology and Environmental Safety, 208, 111655. https://doi.org/10.1016/j.ecoenv.2020.111655.
  8. Doğdu, S. A., Uyan, A., Uygur, N., Gürlek, M., Ergüden, D., & Turan, C. (2016). First record of the Indo-Pacific striped eel catfish, Plotosus lineatus (Thunberg, 1787) from Turkish marine waters. Natural and Engineering Sciences, 1(2), 25-32. https://doi.org/10.28978/nesciences.286245.
  9. Doğdu, S. A., Turan, C., Depci, T., & Ayas, D. (2021). Natural hydroxyapatite obtained from pufferfish teeth for potential dental application. Journal of Ceramic Processing Research, 22(3), 356-361. https://doi.org/10.36410/jcpr.2021.22.3.356.
  10. Ergüden, D., Gurlek, M., Uygur, N., & Turan, C. (2013). Occurrence of fangtooth moray Enchelycore anatina (Lowe, 1839)(Muraenidae) in Iskenderun Bay, Eastern Mediterranean, Turkey. Biharean Biologist, 7(2), 108-110.
  11. Gürlek, M., Ergüden, D., Uyan, A., Doğdu, S. A., Yağl