AI Summary & Key Takeaways
AI InsightMolecular assisted selection was found useful for improving Phytophthora resistant red pepper lines.
📌 3 Key Takeaways
Abstract
Belonging to the Oomycete genus, Phytophthora capsici is responsible of many devastating disease and the most destructive agent of pepper in the World. The most cost-effective method for preventing Phytophthora blight is to use resistant cultivars. In this study, the polygenic resistance of improved pepper lines to Phytophthora Blight were screened by molecular markers placed on three chromosomes in pepper. Sena variety registered as suitable for spice pepper production was used as susceptible female donor and CM 334 and Perennial genotypes were used as male resistance sources. Thirty two genotypes were screened by ASC 035 marker placed on chromosomes P10, ASC 037 on P5 and ASC 031 on P2. Twelve of these improved red pepper lines from different resistance sources were exhibited polygenic resistance according to electrophoretic signals to all of three markers placed on P2, P5 and P10. In order to determine codominance of the resistance genes PCR products of ASC 031 and ASC 037 were restricted by HaeIII and EcoRI respectively. Ten improved lines exhibited codominance related with resistance to Phytophthora Blight concerning ASC 031 and only one line was showed codominance for ASC 037. Three different lines had one resistance gene on P5 and on P10 independently. Only T12 line holds two resistance genes both P2 and P10. DD and 63 lines were most promising genotypes related with their resistance to pathogen. Molecular assisted selection was found useful for improving Phytophthora resistant red pepper lines.