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Journals / Hayvan Bilimi ve Ürünleri Dergisi / 2022 / Cilt: 5 - Sayı: 1

Investigation on the Expression Profile of Immune-Related Genes in Response to Lipopolysaccharide and Lipoteichoic Acid in Alveolar Macrophages of Akkaraman and Romanov Lambs

Akkaraman ve Romanov Kuzularının Alveolar Makrofajlarında Lipopolisakkarit ve Lipoteikoik Asite Yanıtta İmmün İlişkili Genlerin Ekspresyon Profilinin Araştırılması

Ahmed Qasim NAJİ
Md Mahmodul Hasan SOHEL
Saif Adil Abbood AL-JANABİ
Ghulam Asghar SAJİD
Mehmet Ulaş ÇINAR
Pages
7–23
Publication Date
2022-06-27
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that the mRNA expression of immune-related genes was significantly increased in the sheep AMs in response to LPS and LTA treatment whereas a synergistic effect was observed in LPS+LTS treatment.

📌 3 Key Takeaways

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Objective & Hypothesis:The alveolar macrophages (AMs) are frontier of defense against foreign materials that initiate immune response in lungs.
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Key Findings & Data:Results revealed that Romanov lambs exhibited higher mRNA expression of TLR2, TLR4, NF-ĸβ, TNFα, IL-1β, IL-6, IL-8, and IL-10 genes as compared to Akkaraman lambs along with the control of all treatments.
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Conclusion & Impact:The results showed that the mRNA expression of immune-related genes was significantly increased in the sheep AMs in response to LPS and LTA treatment whereas a synergistic effect was observed in LPS+LTS treatment.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:sitokinler · immunogenetik · mRNA ekspresyonu

Abstract

The alveolar macrophages (AMs) are frontier of defense against foreign materials that initiate immune response in lungs. Knowledge of the expression dynamics of major immune-related genes in the alveolar macrophages in response to lipopolysaccharide (LPS) and lipoteichoic acid (LTA) challenge can help to understand disease mechanism involved in several respiratory diseases. The aim of this study was to investigate the mRNA expression of selected immune-related genes in response to lipopolysaccharide (LPS) and lipoteichoic acid (LTA) challenge in sheep alveolar macrophages in vivo. Results revealed that Romanov lambs exhibited higher mRNA expression of TLR2, TLR4, NF-ĸβ, TNFα, IL-1β, IL-6, IL-8, and IL-10 genes as compared to Akkaraman lambs along with the control of all treatments. Moreover, the expression of TLR2, TLR4, NF-ĸβ, TNFα, IL-1β, IL-6, IL-8, and IL-10 genes was higher in combine treatment of LPS and LTA as compared to separate treatments of LPS and LTA in both breeds. The results showed that the mRNA expression of immune-related genes was significantly increased in the sheep AMs in response to LPS and LTA treatment whereas a synergistic effect was observed in LPS+LTS treatment. Also, breed comparison showed that the native Akkaraman was more resistant to disease compared to exotic Romanov.

Özet

Alveolar makrofajlar (AM'lar), akciğerlerde bağışıklık tepkisini başlatan yabancı maddelere karşı savunma bariyeridir. Lipopolisakkarit (LPS) ve lipoteikoik asit (LTA) tehdidine yanıt olarak alveolar makrofajlardaki majör immün bağlantılı genlerin ekspresyon dinamiklerinin bilinmesi, çeşitli solunum yolu hastalıklarında yer alan hastalık mekanizmasının anlaşılmasına yardımcı olabilir. Bu çalışmanın amacı, koyun alveolar makrofajlarında lipopolisakkarit (LPS) ve lipoteikoik asit (LTA) stimülasyonuna yanıt olarak seçilen bağışıklıkla ilgili genlerin mRNA ekspresyonunu in vivo araştırmaktır. Sonuçlar, Romanov kuzularının TLR2, TLR4, NF-ĸβ, TNFa, IL-1β, IL-6, IL-8 ve IL-10 genlerinin mRNA ekspresyonunu Akkaraman kuzularına kıyasla kontrol grubuna göre daha yüksek saptandığını ortaya koymuştur. Ayrıca, her iki ırkta LPS ve LTA'nın kombine uygulanmasında TLR2, TLR4, NF-ĸβ, TNFa, IL-1β, IL-6, IL-8 ve IL-10 genlerinin ekspresyonu, LPS ve LTA'nın ayrı uygulamalarına kıyasla daha yüksektir cinste. Çalışmanın sonuçları, LPS ve LTA uygulamalarına yanıt olarak koyun AM'larında bağışıklıkla ilgili genlerin mRNA ekspresyonunun önemli ölçüde arttığını, LPS+LTS uygulamasında ise sinerjik bir etkinin gözlemlendiğini gösterdi. Ayrıca, ırk karşılaştırması, yerli Akkaraman'ın Romanov'a kıyasla hastalığa karşı daha dirençli olduğunu gösterdi.

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Cite This Article

NAJİ, A. Q., SOHEL, M. M. H., AL-JANABİ, S. A. A., SAJİD, G. A., ÇINAR, M. U. (2022). Investigation on the Expression Profile of Immune-Related Genes in Response to Lipopolysaccharide and Lipoteichoic Acid in Alveolar Macrophages of Akkaraman and Romanov Lambs. Hayvan Bilimi ve Ürünleri Dergisi, Cilt: 5 - Sayı: 1, 7–23. https://ajindex.com/articles/763608

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