Journals / Gaziosmanpaşa Bilimsel Araştırma Dergisi / 2018 / Cilt: 7 - Sayı: 3

Varietal Differences in Antimicrobial Activities of Walnut (Juglans regia L.) Leaf Extracts

Ceviz Yaprak Özütlerinin Antimikrobiyal Aktiviteleri Hususunda Variyatel Farklılıklar

Pages
173–180
Publication Date
2018-12-15
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

MIC values of juglone and the extract for S. aureus bacterium were 0.006 and 0.05 mg/ml, and for C. albicans fungus were 0.003 and 0.025 mg/ml, respectively.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, antibacterial and antifungal activities of leaf extracts of five cultivars of walnut (Juglans regia L.) on eleven bacteria and nine fungi species were determined using agar well diffusion method.
📊
Key Findings & Data:The walnut leaf extracts have generally showed significant antifungal effect on three species (Candida albicans, Botrytis cinerea, Geotrichum candidum) with both ethanolic and water extracts.
💡
Conclusion & Impact:MIC values of juglone and the extract for S. aureus bacterium were 0.006 and 0.05 mg/ml, and for C. albicans fungus were 0.003 and 0.025 mg/ml, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Ceviz yaprak öztüleri · antimikrobiyal aktiviteler · juglon

Abstract

In this study, antibacterial and antifungal activities of leaf extracts of five cultivars of walnut (Juglans regia L.) on eleven bacteria and nine fungi species were determined using agar well diffusion method. Five bacterial species (Pseudomonas gingeri, P. syringae, Staphylococcus aureus, Bacillus cereus and Yersinia enterocolitica) were generally found sensitive to the walnut extracts. However, antibacterial effects of the aqueous extracts were weaker than the ethanolic ones. The most sensitive bacterium to all of the treatments was determined to be S. aureus. The walnut leaf extracts have generally showed significant antifungal effect on three species (Candida albicans, Botrytis cinerea, Geotrichum candidum) with both ethanolic and water extracts. The most sensitive fungus species to all of the treatments was seen to be Candida albicans and the most resistant fungi was Aspergillus niger. Among the walnut cultivars, generally, Şebin cultivar extract was seen to have higher antimicrobial effects than the others. MIC values of juglone and the extract for S. aureus bacterium were 0.006 and 0.05 mg/ml, and for C. albicans fungus were 0.003 and 0.025 mg/ml, respectively.

Özet

Bu çalışmada beş çeşit ceviz (Juglans regia L.) bitkisinin yaprak ekstraktlarının onbir bakteriye ve dokuz mantar türüne karşı antibakteriyel ve antifungal aktiviteleri agar iyi difüzyon yöntemi ile belirlenmiştir. Beş bakteri türü (Pseudomonas gingeri, P. syringae, Staphylococcus aureus, Bacillus cereus ve Yersinia enterocolitica) genellikle ceviz ekstraktlarına duyarlıdır. Bununla birlikte, sulu ekstrelerin antibakteriyel etkileri, etanolik olanlardan daha zayıftı. Tüm tedavilere en duyarlı bakterinin S. aureus olduğu belirlenmiştir. Ceviz yaprağı ekstraktları genel olarak hem etanol hem de su ekstraktlarıyla birlikte üç tür (Candida albicans, Botrytis cinerea, Geotrichum candidum) üzerinde önemli bir antifungal etki göstermiştir. Tüm tedavilere en duyarlı mantar türünün Candida albicans olduğu ve en dirençli mantarların Aspergillus niger olduğu görülmüştür. Ceviz çeşitleri arasında genellikle Şebin çeşidinin, diğerlerine göre daha yüksek antimikrobiyal etkileri olduğu görülmüştür. Juglonun MİK değerleri ve S. aureus bakterisi için ekstrakt 0,006 ve 0,05 mg / ml, C. albicans fungusu sırasıyla 0.003 ve 0.025 mg / ml idi.

🔖

Cite This Article

KOCAÇALIŞKAN, İ., ALBAYRAK, A., İLHAN, S., TERZİ, İ. (2018). Varietal Differences in Antimicrobial Activities of Walnut (Juglans regia L.) Leaf Extracts. Gaziosmanpaşa Bilimsel Araştırma Dergisi, Cilt: 7 - Sayı: 3, 173–180. https://ajindex.com/articles/761140

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (9)

  1. Abou-Zeid, M. A., 2012. "Pathogenic variation in isolates of Pseudomonas causing the brown blotch of cultivated mushroom, Agaricus bisporus." Brazilian Journal of Microbiology 43(3): 1137-1146.
  2. Bai, G. and G. Shaner., 2004. "Management and resistance in wheat and barley to Fusarium head blight." Annual Review Phytopathology, 42: 135-161.
  3. Baudoin, A. and J. Eckert., 1985. "Development of resistance against Geotrichum candidum in lemon peel injuries." Phytopathology 75: 174-179.
  4. Bottone, E. J., 2010. "Bacillus cereus, a volatile human pathogen." Clinical Microbiology Reviews 23(2): 382-398.
  5. Calderone, R. A. and W. A. Fonzi., 2001. "Virulence factors of Candida albicans." Trends Microbiol 9(7): 327-335.
  6. Clark, A. M., T. M. Jurgens and C. D. Hufford., 1990. "Antimicrobial activity of juglone." Phytotherapy Research 4(1): 11-14. CLSI., 2010. Reference method for antifungal disk diffusion susceptibility testing of non-dermatophyte filamentous fungi; Approved guideline CLSI document M51-A. Clinical and Laboratory Standards Institute, Villanova, 15 PA. CLSI., 2012a. Performance Standards for Antimicrobial Disk Susceptibility Tests, Approved Standard. CLSI document M02-A11. Clinical and Laboratory Standards Institute, USA. CLSI (2012b). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. CLSI document M07-A9. Clinical and Laboratory Standards Institute, USA. Cowan, M. M., 1999. "Plant products as antimicrobial agents." Clinical Microbiology Reviews 12(4): 564. Daferera, D. J., B. N. Ziogas and M. G. Polissiou (2003). "The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. michiganensis." Crop Protection 22(1): 39-44.
  7. Daglish, C., 1950. "The isolation and identification of a hydrojuglone glycoside occurring in the walnut." Biochemical Journal 47(4): 452. Dakshini, K. and C. L. Foy., 1999. Principles and practices in plant ecology: Allelochemical Interactions, CRC press. Dama, L., B. Poul and B. Jadhav., 1998. "Antimicrobial activity of naphthoquinonic compounds." Journal of Ecotoxicology & Environmental Monitoring 8(3): 213-215.
  8. Govrin, E. M. and A. Levine., 2000. "The hypersensitive response facilitates plant infection by the necrotrophic pathogen Botrytis cinerea." Current Biology 10(13): 751-757.
  9. Huovinen, E., L. M. Sihvonen, M. J. Virtanen, K. Haukka, A. Siitonen and M. Kuusi., 2010. "Symptoms and sources of Yersinia enterocolitica-infection: a case-c