SavedAdd your journal

Journals / Anatolian Current Medical Journal / 2021 / Cilt: 3 - Sayı: 2

The effects of folic acid on vascular reactivity in a hyperhomocysteinemic rat model

Melek YILMAZ
Hüseyin GEMALMAZ
Cihan YÜCEL
Serkan KETENCİLER
Uğur GÜRCÜN
Berent DİŞÇİGİL
Pages
151–157
Publication Date
2021-04-24
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Keywords: Hyperhomocysteinemia, folic acid, vascular reactivity

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Abstract Objective: This study aimed to investigate the antioxidant effects of folic acid and its effects on contraction and relaxation responses in rat aorta in hyperhomocysteinemic rats.
📊
Key Findings & Data:Rats in the hyperhomocysteinemia group (Group 1, n=9) received 1g/kg/day methionine via orogastric gavage for 30 days and then injected with intraperitoneal saline for the next 7 days.
💡
Conclusion & Impact:Keywords: Hyperhomocysteinemia, folic acid, vascular reactivity
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Hyperhomocysteinemia · folic acid · vascular reactivity

Abstract

Abstract Objective: This study aimed to investigate the antioxidant effects of folic acid and its effects on contraction and relaxation responses in rat aorta in hyperhomocysteinemic rats. Methods: Thirty-four male Wistar Albino rats were allocated into four groups. Rats in the hyperhomocysteinemia group (Group 1, n=9) received 1g/kg/day methionine via orogastric gavage for 30 days and then injected with intraperitoneal saline for the next 7 days. In the hyperhomocysteinemia+folic acid group (Group 2, n=9), following the administration of methionine for 30 days, folic acid (4mg/kg/day) was delivered intraperitoneally for 7 days. Sham group rats (Group 3, n=8) received orogastric saline for 30 days, which was followed by an IP injection of saline for another 7 days. Animals allocated into the folic acid group (Group 4, n= 7) had orogastric saline for 30 days and intraperitoneal folic acid for 7 days. After 5 weeks of treatment, blood samples were obtained, all animals were sacrificed, and hearts were harvested. Thoracic aortic segments were suspended on individual organ baths, and acetylcholine-induced (endothelium-dependent) relaxation responses of isolated aortic rings were evaluated. Results: Relaxation responses in Group 1 thru 4 were 73.889.96, 76.159.28, 76.618.83, and 69.2615.68, respectively. There was no significant difference in the organ bath in terms of relaxation response to acetylcholine at a dose of 10-9 mM between the groups (F=0.716, p=0.550). Conclusion: Folic acid therapy failed to produce a significant improvement in vascular reactivity. Keywords: Hyperhomocysteinemia, folic acid, vascular reactivity

🔖

Cite This Article

YILMAZ, M., GEMALMAZ, H., YÜCEL, C., KETENCİLER, S., GÜRCÜN, U., DİŞÇİGİL, B. (2021). The effects of folic acid on vascular reactivity in a hyperhomocysteinemic rat model. Anatolian Current Medical Journal, Cilt: 3 - Sayı: 2, 151–157. https://ajindex.com/articles/733843

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Onat A, Can G. Prevalence of heart disease in our adults, new coronary events and prevalence of death from heart. TEKHARF; 2017.
  2. Pahwa R, Jialal I. Atherosclerosis. StatPearls. Treasure Island (FL). StatPearls Publishing; 2018.
  3. Ducker GS, Rabinowitz JD. One-Carbon Metabolism in Health and Disease. Cell Metab 2017; 25: 27–42. de Benoist B. Conclusions of a WHO Technical Consultation on folate and vitamin B12 deficiencies. Food Nutr Bull 2008; 29: S238–44. Tinelli C, Di Pino A, Ficulle E, Marcelli S, Feligioni M. Hyperhomocysteinemia as a risk factor and potential nutraceutical target for certain pathologies. Front Nutr 2019; 6: 49. Cui S, Li W, Wang P, Lv X, Gao Y, Huang G. Folic acid inhibits homocysteine-induced cell apoptosis in human umbilical vein endothelial cells. Mol Cell Biochem 2018; 444: 77–86.
  4. McDowell IF, Lang D. Homocysteine and endothelial dysfunction: a link with cardiovascular disease. J Nutr 2000; 130: 369-372.
  5. Ledingham M, Thomson AJ, Greer IA, Norman JE. Nitric oxide in parturition. BJOG An Int J Obstet Gynaecol 2000; 107: 581–93.
  6. Ungvari Z, Pacher P, Rischák K, Szollár L, Koller A. Dysfunction of nitric oxide mediation in isolated rat arterioles with methionine diet-induced hyperhomocysteinemia. Arterioscler Thromb Vasc Biol 1999; 19: 1899–904.
  7. Chauveau P, Chadefaux B, Coudé M, Aupetit J, Kamoun P, Jungers P. Long-term folic acid (but not pyridoxine) supplementation lowers elevated plasma homocysteine level in chronic renal failure. Miner Electrolyte Metab 1996; 22: 106-9.
  8. Li N, Chen Y-F, Zou A-P. Implications of hyperhomocysteinemia in glomerular sclerosis in hypertension. Hypertens (Dallas, Tex 1979) 2002; 39: 443–8.
  9. Burtis CA, Ashwood ER. Tietz textbook of clinical chemistry. Philadelphia 1999; 1999: 1654–5.
  10. Cortas NK, Wakid NW. Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clin Chem 1990; 36: 1440–3.
  11. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497–500.
  12. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 158–69.
  13. Carlberg I, Mannervik B. Glutathione reductase. Methods Enzymol 1985; 113: 484–90. Aebi H. Catalase in vitro. Methods Enzymol 1984; 105: 121–6.
  14. Discigil B, Evora PRB, Pearson PJ, Viaro F, Rodrigues AJ, Schaff H V. Ionic radiocontrast inhibits endothelium-dependent vasodilation of the canine renal artery in vitro: possible m