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Journals / Türkiye Entomoloji Dergisi / 2022 / Cilt: 46 - Sayı: 1

Characterization and taxonomic utility of ITS2 in Dolerus Panzer, 1801 (Hymenoptera: Tenthredinidae)

Dolerus Panzer, 1801 (Hymenoptera: Tenthredinidae) cinsinde ITS2'nin karakterizasyonu ve taksonomik kullanımı

Mehmet GÜLMEZ
Mahir BUDAK
Ertan Mahir KORKMAZ
Sevda HASTAOĞLU ÖRGEN
Hasan Hüseyin BAŞIBÜYÜK
Pages
13–23
Publication Date
2022-03-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Four species (Dolerus triplicatus (Klug, 1818), Dolerus germanicus (Fabricius, 1775), Dolerus puncticollis Thomson, 1871 and Dolerus nigratus (Müller, 1776) and two putative species (Dolerus spp. 1 and 2) were determined from 36 individuals belonging to Dolerus.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The widespread use of ITS2 as a potential marker in insects has accelerated species-level phylogenetic studies.
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Key Findings & Data:This paper presents the phylogenetic relationship among 36 individuals of Dolerus Panzer, 1801 (Hymenoptera: Tenthredinidae) collected between 2002 and 2018 to obtain the first data on ITS2 secondary structure.
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Conclusion & Impact:Four species (Dolerus triplicatus (Klug, 1818), Dolerus germanicus (Fabricius, 1775), Dolerus puncticollis Thomson, 1871 and Dolerus nigratus (Müller, 1776) and two putative species (Dolerus spp. 1 and 2) were determined from 36 individuals belonging to Dolerus.
🔬Design:Research Article
📍Scope:2002 Verileri
⚙️Variables:CBC · Dolerus · Hymenoptera

Abstract

The widespread use of ITS2 as a potential marker in insects has accelerated species-level phylogenetic studies. Reliable and quality data can be obtained thanks to the features such as rapid evolution and secondary structure of this marker. This paper presents the phylogenetic relationship among 36 individuals of Dolerus Panzer, 1801 (Hymenoptera: Tenthredinidae) collected between 2002 and 2018 to obtain the first data on ITS2 secondary structure. Aligned of ITS2 data were analyzed by application of maximum likelihood method to reveal phylogenetic relationship among the specimens. Also, the structural properties, length variation and presence of compensatory base changes make the ITS2 useful marker in determination of species boundaries of closely related species. Four species (Dolerus triplicatus (Klug, 1818), Dolerus germanicus (Fabricius, 1775), Dolerus puncticollis Thomson, 1871 and Dolerus nigratus (Müller, 1776) and two putative species (Dolerus spp. 1 and 2) were determined from 36 individuals belonging to Dolerus.

Özet

Böceklerde ITS2'nin moleküler belirteç olarak kullanılmasının yaygınlaşması, tür düzeyindeki filogeni çalışmalarına hız kazandırmıştır. Bu belirtecin hem hızlı evrime sahip olması hem de ikincil yapı oluşturması gibi özellikler sayesinde güvenilir ve kaliteli veriler elde edilebilir. Bu makalede, Dolerus Panzer, 1801 cinsine ait 2002-2018 yılları arasında toplanmış olan 36 bireyin (Hymenoptera: Tenthredinidae) aralarındaki filogenetik ilişkiyi ve ITS2 ikincil yapı özelliklerine ilişkin ilk verileri sunuyoruz. ITS2'nin hizalanmış verileri, örnekler arasındaki ilişkiyi görmek için maksimum likelihood yöntemi uygulanarak analiz edildi. Aynı zamanda ITS2'nin yapısal özellikleri, uzunluk polimorfizmi ve CBC'lerin olması, yakından ilişkili türlerin tür sınırlarının belirlenmesinde faydalıdır, Dolerus cinsine ait 36 bireyden, 4 tür (Dolerus triplicatus (Klug, 1818), Dolerus germanicus (Fabricus, 1775), Dolerus puncticollis Thomson, 1871 ve Dolerus nigratus (Müller, 1776) ve 2 olası tür (Dolerus spp. 1 ve 2) belirlenmiştir.

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Cite This Article

GÜLMEZ, M., BUDAK, M., KORKMAZ, E. M., ÖRGEN, S. H., BAŞIBÜYÜK, H. H. (2022). Characterization and taxonomic utility of ITS2 in Dolerus Panzer, 1801 (Hymenoptera: Tenthredinidae). Türkiye Entomoloji Dergisi, Cilt: 46 - Sayı: 1, 13–23. https://ajindex.com/articles/724674

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References (10)

  1. Aljanabi, S. M. & I. Martinez, 1997. Universal and rapid salt-extraction of high-quality genomic DNA for PCR-based techniques. Nucleic Acids Research, 25 (22): 4692-4693.
  2. Ankenbrand, M. J., A. Keller, M. Wolf, J. Schultz & F. Förster, 2015. ITS2 database V: twice as much. Molecular Biology & Evolution, 32 (11): 3030-3032.
  3. Barker, A. M., 1998. The identification of larvae of eight graminivorous species of the sawfly genus Dolerus Panzer 1801 (Hymenoptera: Tenthredinidae) regularly found in grass and cereal fields in southern England. Journal of Natural History, 32 (8): 1181-1215.
  4. Barker, A. M., N. J., Brown & C. J. M. Reynolds 1999. Do host-plant requirements and mortality from soil cultivation determine the distribution of graminivorous sawflies on farmland? Journal of Applied Ecology, 36 (2): 271-282.
  5. Budak, M., M. Güler, E. M. Korkmaz, S. H. Örgen & H. H. Başıbüyük, 2016. The characterisation and taxonomic utility of ITS2 in Tenthredopsis Costa, 1859 (Tenthredinidae: Hymenoptera) with some new records from Turkey. Biochemical Systematics & Ecology, 66: 76-85. Caisová L., B. Marin & M. Melkonian, 2011. A close-up view on ITS2 evolution and speciation-a case study in the Ulvophyceae (Chlorophyta, Viridiplantae). BMC Evolutionary Biology, 11 (1): 1-24. Caisová, L., B. Marin & M. A. Melkonian, 2013. A consensus secondary structure of ITS2 in the Chlorophyta identified by phylogenetic reconstruction. Protist, 164 (4): 482-496. Caisová, L. & M. Melkonian, 2014. Evolution of helix formation in the ribosomal internal transcribed spacer 2 (ITS2) and its significance for RNA secondary structures. Journal of Molecular Evolution, 78 (6): 324-337.
  6. Çalmaşur, Ö. & H. Özbek, 2004a. A contribution to the knowledge of the Tenthredinidae (Symphyta, Hymenoptera) Fauna of Turkey Part I: The Subfamily Tenthredininae. Turkish Journal of Zoology, 28 (1): 37-54.
  7. Çalmaşur, Ö. & H. Özbek, 2004b. Contribution to the knowledge of Tenthredinidae (Symphyta, Hymenoptera) fauna of Turkey Part II: Subfamilies Blennocampinae, Dolerinae, Nematinae and Selandrinae. Turkish Journal of Zoology, 28 (1): 55-71.
  8. Çalmaşur, Ö., 2020. New records and some new distribution data for the Turkish Nematinae (Hymenoptera: Symphyta: Tenthredinidae) fauna. Turkish Journal of Entomology, 44 (3): 413-422.
  9. Coleman, A. W., 2003. ITS2 is a double-edged tool for eukaryote evolutionary comparisons. Trends in Genetics, 19 (7): 370-375.
  10. Coleman, A. W., 2007. Pan-eukaryote ITS2 homologies revealed by RNA secondary s