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Journals / Türkiye Entomoloji Dergisi / 2026 / Cilt: 50 Sayı: 3

Comparison of hemolymph biochemical parameters, productive performance and structural properties of silk fibers of silkworm, Bombyx mori L., 1758 (Lepidoptera: Bombycidae) by dietary diversification

Beslenme çeşitliliği ile ipek böceğinin, Bombyx mori L., 1758 (Lepidoptera: Bombycidae) hemolenf biyokimyasal parametreleri, üretim performansı ve ipek liflerinin yapısal özelliklerinin karşılaştırılması

Şeniz Öziş Altınçekiç(Bursa Uludağ Üniversitesi)0000-0001-9044-8092
Arda Çelik(BURSA ULUDAG UNIVERSITY)0000-0002-0955-4371
Pages
327–340
Publication Date
2026-09-09
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Fourier-transform infrared spectroscopy analysis revealed differences in the transmittance characteristics of cocoon shell and silk fibers between the feeding groups.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to compare the effects of dietary diversification of Bombyx mori L., 1758 (Lepidoptera: Bombycidae) larvae on hemolymph biochemical parameters, larval growth parameters, and cocoon and silk morphological and structural properties.
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Key Findings & Data:The newly hatched silkworm larvae of a hybrid line (M × N) were randomly separated into two feeding groups: (1) mulberry leaves (ML), (2) artificial diet (AD).
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Conclusion & Impact:Fourier-transform infrared spectroscopy analysis revealed differences in the transmittance characteristics of cocoon shell and silk fibers between the feeding groups.
🔬Design:Araştırma Makalesi
📍Scope:2025 Verileri
⚙️Variables:Yapay diyet · larval büyüme · ipek böceği

Abstract

The aim of this study was to compare the effects of dietary diversification of Bombyx mori L., 1758 (Lepidoptera: Bombycidae) larvae on hemolymph biochemical parameters, larval growth parameters, and cocoon and silk morphological and structural properties. The newly hatched silkworm larvae of a hybrid line (M × N) were randomly separated into two feeding groups: (1) mulberry leaves (ML), (2) artificial diet (AD). The experiment was conducted at Kozabirlik (Bursa, Türkiye) in May 2025 under controlled rearing conditions. The larval period was shorter, and larval weight and silk gland weight were higher in the ML group compared with the AD group ( p< 0.001). The silk gland pH was determined to be lower in the ML group. The amylase activity was significantly higher in the ML group than in the AD group ( p= 0.002), whereas urease activity was higher in the AD group than in the ML group ( p< 0.001). The pupal, cocoon and filament characteristics were found to be significantly higher in the ML group ( p< 0.001). Furthermore, scanning electron microscopy analysis revealed a larger cocoon and silk fiber diameters in larvae fed with ML ( p< 0.01). Fourier-transform infrared spectroscopy analysis revealed differences in the transmittance characteristics of cocoon shell and silk fibers between the feeding groups.

Özet

Bu çalışmanın amacı, beslenme farklılığının Bombyx mori L., 1758 (Lepidoptera: Bombycidae) larvalarında hemolenf biyokimyasal parametreleri, larva gelişim özellikleri, koza ve ipek morfolojik ve yapısal özellikleri üzerindeki etkilerini karşılaştırmalı olarak değerlendirmektir. Yumurtadan yeni çıkan hibrit hatta (M × N) ait ipek böceği larvaları rastgele iki besleme grubuna ayrılmıştır: (1) dut yaprakları (DY) (2) yapay mama (YM) ile besleme. Deneme, Mayıs 2025 tarihinde Kozabirlik (Bursa, Türkiye) bünyesinde kontrollü yetiştirme koşullarında gerçekleştirilmiştir. DY grubunda larva gelişim süresi daha kısa bulunurken, larva ağırlığı ve ipek bezi ağırlığı AD grubuna kıyasla daha yüksek bulunmuştur ( p< 0,001). İpek bezi pH değeri DY grubunda daha düşük belirlenmiştir. Amilaz aktivitesi DY grubunda, YM grubuna göre anlamlı derecede yüksek bulunmuştur ( p= 0,002). Buna karşılık, üreaz aktivitesi YM grubunda, DY grubuna göre daha yüksek bulunmuştur ( p< 0,001). Pupa, koza ve filament özellikleri DY grubunda anlamlı derecede daha yüksek bulunmuştur ( p< 0,001). Ayrıca, taramalı elektron mikroskobu (SEM) analizleri, DY ile beslenen larvalardan elde edilen koza ve ipek liflerinin çaplarının daha yüksek olduğunu göstermiştir ( p< 0,01). Fourier dönüşümlü kızılötesi spektroskopi (FTIR) analizleri, besleme grupları arasında koza kabuğu ve ipek liflerinin geçirgenlik (transmittance) özelliklerinde farklılıklar olduğunu ortaya koymuştur.

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Cite This Article

Altınçekiç, Ş. Ö., Çelik, A. (2026). Comparison of hemolymph biochemical parameters, productive performance and structural properties of silk fibers of silkworm, Bombyx mori L., 1758 (Lepidoptera: Bombycidae) by dietary diversification. Türkiye Entomoloji Dergisi, Cilt: 50 Sayı: 3, 327–340. https://doi.org/10.16970/entoted.1793977

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