DNA-Binding Properties of Nickle II Phthalocyanine with 2-Isopropyl-5-Methylphenoxy Substituents and Their Studies of UV/Vis, Fluorescence Spectroscopies, Cyclic voltametry, Gel Eelectrophoresis and Viscosity Measurement
The results showed that Ni II phthalocyanine compound binds strongly to calf thymus-DNA via intercalation binding
📌 3 Key Takeaways
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Objective & Hypothesis:I n this study, previously synthesized Ni II phthalocyanine compound bearing 2,10,16,24-tetrakis 2-isopropyl-5-methylphenoxy group was chosen for its interaction with calf thymus-DNA.
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Key Findings & Data:The DNA binding activities of Ni II phthalocyanine compound bearing 2-isopropyl-5-methylphenoxy substituent was investigated by using absorption titration, fluorescence emission, cyclic voltammetry, gel elctrophoresis in Tris-HCl buffer at pH 7.0.
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Conclusion & Impact:The results showed that Ni II phthalocyanine compound binds strongly to calf thymus-DNA via intercalation binding
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Uv-Vis spectroscopy · DNA binding · phthalocyanines
Abstract
I n this study, previously synthesized Ni II phthalocyanine compound bearing 2,10,16,24-tetrakis 2-isopropyl-5-methylphenoxy group was chosen for its interaction with calf thymus-DNA. Calf thymus-DNA was used to determine DNA binding properties of Ni II phthalocyanine compound. The DNA binding activities of Ni II phthalocyanine compound bearing 2-isopropyl-5-methylphenoxy substituent was investigated by using absorption titration, fluorescence emission, cyclic voltammetry, gel elctrophoresis in Tris-HCl buffer at pH 7.0. In addition to above methods, melting point and viscosity experiment were performed to determine the DNA intercation of the compound in Tris-HCl buffer solution. The results showed that Ni II phthalocyanine compound binds strongly to calf thymus-DNA via intercalation binding
ARSLANTAS, A., AGİRTAS, M. S. (2017). DNA-Binding Properties of Nickle II Phthalocyanine with 2-Isopropyl-5-Methylphenoxy Substituents and Their Studies of UV/Vis, Fluorescence Spectroscopies, Cyclic voltametry, Gel Eelectrophoresis and Viscosity Measurement. Hittite Journal of Science and Engineering, Cilt: 4 - Sayı: 2, 91–97. https://ajindex.com/articles/671650
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