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Journals / Hittite Journal of Science and Engineering / 2019 / Cilt: 6 - Sayı: 1

Evaluation of Angiogenic Factor Release From Thermosensitive Poly N-Vinylcaprolactam -g-Collagen: In Vitro and In Vivo Studies

Serap DURKUT
Pages
57–61
Publication Date
2019-03-28
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The results indicated that PNVCL-g-Col hydrogel preserved its stability and released ~90% of the loaded VEGF within this time period.

📌 3 Key Takeaways

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Objective & Hypothesis:I n this study, a thermosensitive poly N-vinylcaprolactam -g-collagen PNVCL-g-Col hybrid hydrogel was synthesized by conjugation using the NHS/EDC cross-linking system, and characterized.
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Key Findings & Data:At first, the efficiency of in vitro sustained delivery of human vascular endothelial growth factor VEGF from the thermosensitive PNVCL-g-Col hydrogel modified with heparin, was evaluated for duration of ten days under in vitro physiological conditions 37oC, pH 7.4 .
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Conclusion & Impact:The results indicated that PNVCL-g-Col hydrogel preserved its stability and released ~90% of the loaded VEGF within this time period.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Thermosensitive polymer · Sustained release · Conjugation

Abstract

I n this study, a thermosensitive poly N-vinylcaprolactam -g-collagen PNVCL-g-Col hybrid hydrogel was synthesized by conjugation using the NHS/EDC cross-linking system, and characterized. At first, the efficiency of in vitro sustained delivery of human vascular endothelial growth factor VEGF from the thermosensitive PNVCL-g-Col hydrogel modified with heparin, was evaluated for duration of ten days under in vitro physiological conditions 37oC, pH 7.4 . The results indicated that PNVCL-g-Col hydrogel preserved its stability and released ~90% of the loaded VEGF within this time period. In vitro study showed that PNVCL-g-Col was basically histocompatible. Then, the in vivo angiogenic activity of the VEGF-releasing PNVCL-g-Col was investigated using a subcutaneous rat model. In vivo study confirmed that angiogenic-factor-loaded PNVCL-g-Col had the capacity to induce neovascularization indicating that the in vivo bioactivity of the VEGF was preserved in the thermosensitive PNVCL-g-Col

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Cite This Article

DURKUT, S. (2019). Evaluation of Angiogenic Factor Release From Thermosensitive Poly N-Vinylcaprolactam -g-Collagen: In Vitro and In Vivo Studies. Hittite Journal of Science and Engineering, Cilt: 6 - Sayı: 1, 57–61. https://ajindex.com/articles/671627

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References (15)

  1. Hoffman AS. Stimuli-responsive polymers: Biomedical applications and challenges for clinical translation. Adv. Drug Deliv. Rev., 65, 10–16, 2013
  2. Qiu Y, Park K. Environment-sensitive hydrogels for drug delivery. Adv. Drug Deliv. Rev., 64, 49–60, 2012
  3. Peppas NA, Hilt JZ, Khademhosseini A. Hydrogels in biology and medicine: from molecular principles to bionanotechnology. Adv Mater;18:1345–60, 2006
  4. Jeong B, Kim SW, Bae YH. Rev. Thermosensitive Sol−Gel Reversible Hydrogels. Adv. Drug Delivery, 64 (Supplement), 154−162, 2012
  5. Cortez-Lemus NA, Licea-Claverie A. Poly(Nvinylcaprolactam), a comprehensive review on a thermoresponsive polymer becoming popular. Prog Polym Sci 53,1, 2016
  6. Ferraz CC, Varca GHCC, Ruiz J, Lopes PS, Mathor MB, Lugão AB, Bucio E. Radiation-grafting of thermo- and pH-responsive poly(N-vinylcaprolactam-coacrylic acid) onto silicone rubber and polypropylene films for biomedical purposes, Radiat. Phys. Chem. 97, 298–303, 2014
  7. Gillette BM, Jensen JA, Tang B. In situ collagen assembly for integrating microfabricated threedimensional cell-seeded matrices. Nat. Mater. 7(8):636–640, 2008
  8. Klouda L, Mikos AG. Thermoresponsive hydrogels in biomedical applications. Eur. J. Pharm. Biopharm. 68, 34–45, 2008
  9. Lee SC, Kwon IK, Park K. Hydrogels for delivery of bioactive agents: A historical perspective. Adv Drug Deliv Rev; 65:17–20, 2013
  10. Chu H, and Wang Y. Therapeutic angiogenesis: controlled delivery of angiogenic factors. Ther. Deliv. 3, 693–714, 2012
  11. Gu C, Zheng R, Yang Z, Wen A, Wu H, Zhang H, Yi D. Novel glycidyl methacrylated dextran/ gelatin nanoparticles loaded with basic fibroblast growth factor: Formulation and characteristics. Drug Dev Ind Pharm;35: 1419–142, 2009
  12. Prabaharan M, Grailer JJ, Steeber DA, Gong S. Stimuli-Responsive Chitosan-graft-Poly(Nvinylcaprolactam) as a Promising Material for Controlled Hydrophobic Drug Delivery. Macromol. Biosci., 8, 843–851, 2008
  13. Liu J, Debuigne A, Detrembleur C, Jerome C. Poly(Nvinylcaprolactam): a thermoresponsive macromolecule with promising future in biomedical field. Adv Healthc Mater. 3, 1941– 68, 2014
  14. Wu JY, Liu SQ, Heng PWS, Yang YY. Evaluating proteins release from, and their interactions with, thermosensitive poly (N-isopropylacrylamide) hydrogels. J Control Release. 102:361–372, 2005
  15. Durkut S, Elçin YM. Synthesis and characterization of thermosensitive poly(N-vinylcaprolactam)-g-collagen. Artificial Cells, Nanomedicine, and Biotechnology,