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Journals / Hacettepe Journal of Biology and Chemistry / 2022 / Cilt: 50 - Sayı: 4

How mesenchymal stem cell conditioned media affect the HeLa cells on Wnt/beta-catenin signaling, Notch-1 signaling, and apoptosis?

Hanife Guler DONMEZ
Handan SEVİM AKAN
Pages
367–375
Publication Date
2022-10-09
DOI
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⚡ TL;DR(Too Long; Didn't Read)

MTT cell viability, AO/PI, and immunocytochemical staining of cleaved caspase 3 results showed that through all incubation periods, there was no statistically significant difference between the MSCs-CM treated HeLa cells and the controls (p>0.05).

📌 3 Key Takeaways

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Objective & Hypothesis:This study aims to investigate the influence of mesenchymal stem cells (MSCs) cell-conditioned media (MSCs-CM) on the Wnt/beta-catenin and Notch-1 signaling as well as the apoptosis in cervical cancer cells.
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Key Findings & Data:HeLa cells cultured standard conditions and treated with MSCs-CM 24, 48, 72 hours.
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Conclusion & Impact:MTT cell viability, AO/PI, and immunocytochemical staining of cleaved caspase 3 results showed that through all incubation periods, there was no statistically significant difference between the MSCs-CM treated HeLa cells and the controls (p>0.05).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Mesenchymal stem cell · Beta-catenin · Notch-1

Abstract

This study aims to investigate the influence of mesenchymal stem cells (MSCs) cell-conditioned media (MSCs-CM) on the Wnt/beta-catenin and Notch-1 signaling as well as the apoptosis in cervical cancer cells. Conditioned media of characterized MSCs were freshly collected and filtered before use. HeLa cells cultured standard conditions and treated with MSCs-CM 24, 48, 72 hours. Untreated cells serve as a control. Cell viability measured with MTT assay for all incubation periods. Immunocytochemical staining of beta-catenin, Notch-1 and cleaved caspase 3 were performed for each time-point. MTT cell viability, AO/PI, and immunocytochemical staining of cleaved caspase 3 results showed that through all incubation periods, there was no statistically significant difference between the MSCs-CM treated HeLa cells and the controls (p>0.05). Beta-catenin immunoreactivity was upregulated following treatment from 24 hours to 48 and 72 hours (p

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Cite This Article

DONMEZ, H. G., AKAN, H. S. (2022). How mesenchymal stem cell conditioned media affect the HeLa cells on Wnt/beta-catenin signaling, Notch-1 signaling, and apoptosis?. Hacettepe Journal of Biology and Chemistry, Cilt: 50 - Sayı: 4, 367–375. https://ajindex.com/articles/669120

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